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Addgene

pGL0_115 [pBC1 ORI]
(Plasmid #199015)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199015 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGLDV_17
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E. coli Twist 10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pBC1 ORI
  • Species
    Bacillus coagulans

Cloning Information

  • Cloning method Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Riccardi Lab (De Rossi et al. 1992 https://doi.org/10.1128/jb.174.2.638-642.1992)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL0_115 [pBC1 ORI] was a gift from Charlie Gilbert & Nili Ostrov & Cultivarium Tools (Addgene plasmid # 199015 ; http://n2t.net/addgene:199015 ; RRID:Addgene_199015)
  • For your References section:

    A scalable framework for high-throughput identification of functional origins of replication in non-model bacteria. Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z, Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N. bioRxiv 2023.05.19.541510 10.1101/2023.05.19.541510