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Showing: 1 - 50 of 464 results
  1. lentiCas9-Blast (Lentiviral Prep)

    Viral Prep
    #52962-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from lentiCas9-Blast (#52962). In addition to the viral particles, you will also receive purified lentiCas9-Blast plasmid DNA. Lentiviral particles carrying Cas9 and blasticidin resistance. This virus is used to make stable cell lines expressing Cas9.
    Depositor
    Promoter
    EFS-NS
    Tags
    None
    Available since
    July 14, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. pLVX-Tet3G blasticidin

    Plasmid
    #128061
    Purpose
    Lentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pUt-mNF-BACH1 Cas9-Resistant

    Plasmid
    #199218
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Negative-Feedback circuit (mNF-BACH1 Cas9- resistant). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Insert
    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    silient mutation at nucleic acid 177 C changed to…
    Promoter
    CMV D2ir promoter
    Available since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. SRP19 (siRNA resistant) in pLX311

    Plasmid
    #233291
    Purpose
    Expression of WT SRP19 (siRNA resistant)
    Depositor
    Insert
    SRP19 (SRP19 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Expresses WT SRP19 that is resistant to siRNA
    Promoter
    E1Fa
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato

    Plasmid
    #172535
    Purpose
    Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistance
    Depositor
    Insert
    -3.5ubb:plin2-tdtomato
    Use
    Tags
    Expression
    Mutation
    S80R, A81S, E144D, I148F and R415H mutations obse…
    Promoter
    zebrafish ubb
    Available since
    Sept. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pUt-mPF-BACH1 Cas9-Resistant_AmpR

    Plasmid
    #199221
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Insert
    rtTA-Advanced::P2A::EGFP::BACH1 (BACH1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    silient mutation at nucleic acid 177 C changed to…
    Promoter
    tight TRE promoter
    Available since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin

    Plasmid
    #187952
    Purpose
    FKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Insert
    FKBP12_F36V-CasRx-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker, HA-tag, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187945
    Purpose
    dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Insert
    SpdCas9-tagRFPt-P2A-tagBFP
    Use
    Synthetic Biology; Piggybac
    Tags
    NLS, NLS + HA-tag, P2A, tagBFP, and tagRFPt
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid
    #187956
    Purpose
    FKBP12 (F36V mutant) degron-tagged hHDAC4-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Insert
    FKBP12_F36V-hHDAC4-SpdCas9-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-Blasticidin

    Plasmid
    #187953
    Purpose
    FKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Insert
    FKBP12_F36V-SpdCas9-KRAB-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid
    #187957
    Purpose
    FKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Insert
    FKBP12_F36V-KRAB-SpdCas9-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. pSLPB2B-FKBP12_F36V-SpdCas9-hHDAC4-tagBFP-PGK-Blasticidin

    Plasmid
    #187954
    Purpose
    FKBP12 (F36V mutant) degron-tagged dCas9-hHDAC4 fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Insert
    FKBP12_F36V-SpdCas9-hHDAC4-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187950
    Purpose
    ecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Insert
    ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  14. pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187948
    Purpose
    ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Insert
    ER50-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Mutation
    6 mutations, T371A, L384M, M421G, G521R, Y537S, N…
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  15. pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187951
    Purpose
    FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Insert
    FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  16. pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187949
    Purpose
    SMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Insert
    SMASh-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  17. lentiCas9-Blast

    Plasmid
    #52962
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.
    Depositor
    Has Service
    Lentiviral Prep
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
  18. pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

    Plasmid
    #187960
    Purpose
    AID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.
    Depositor
    Insert
    AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  19. pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

    Plasmid
    #187963
    Purpose
    mAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.
    Depositor
    Inserts
    AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
    osTIR1
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker and T2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  20. lentiCas9n(D10A)-Blast

    Plasmid
    #63593
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, nickase mutant
    Promoter
    EFS-NS
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
  21. pCWX-R4DESTR2-PGK-BSD

    Plasmid
    #114320
    Purpose
    Lentiviral backbone for Gateway cloning of Promoter/Gene insert together with constitutive Blasticidin resistance
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  22. pLVU/myc

    Plasmid
    #24179
    Depositor
    Insert
    Chloramphenicol resistance gene (CmR) - ccdB gene
    Use
    Lentiviral; Gateway
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 29, 2010
    Availability
    Academic Institutions and Nonprofits only
  23. pBS SK mCherryROSAbsr

    Plasmid
    #54321
    Purpose
    Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.
    Insert
    mCherry and BlasticidinS-resistance
    Use
    CRISPR, Mouse Targeting, and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
  24. pBSVSG221KOblast

    Plasmid
    #59733
    Purpose
    For replacement of VSG221 with a blasticidin resistence gene in the VSG221 expression site in T.brucei.
    Depositor
    Insert
    VSG221 knockout construct
    Use
    For homologous recombination into vsg221 expressi…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 15, 2015
    Availability
    Academic Institutions and Nonprofits only
  25. pDONR221_SMAD2_HAe2_Bsd

    Plasmid
    #167166
    Purpose
    Blasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.
    Depositor
    Insert
    Repair template to knockout SMAD2 by inserting a blasticidin resistance cassette (SMAD2 Human)
    Use
    Repair template for gene editing in human cells
    Tags
    blasticidin
    Expression
    Mutation
    Promoter
    Available since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
  26. pLentiGuide-BlastR

    Plasmid
    #196970
    Purpose
    Lentiviral acceptor vector for pegRNAs with blasticidin resistance
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
  27. pENTR-R2L3-IRES-BSD-pA

    Plasmid
    #139534
    Purpose
    Provides IRES-BSD-pA cassette on Gateway entry vector
    Depositor
    Insert
    Blasticidin resistant gene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
  28. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid
    #106477
    Purpose
    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
  29. mCerulean3-Bik-s2193

    Plasmid
    #177425
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bik, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.
    Depositor
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    mCerulean3
    Expression
    Mammalian
    Mutation
    Promoter
    Human ferritin
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  30. OSC_Reporter_UAS_traffic jam_BoxB_d2eGFP_t2a_Blast

    Plasmid
    #128010
    Purpose
    traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites
    Depositor
    Insert
    traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites
    Use
    Drosophila osc reporter construct
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 25, 2019
    Availability
    Academic Institutions and Nonprofits only
  31. pSLQ2818_Blasti_pPB: CAG-PYL1-KRAB-IRES-Blasti-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9-FKBP_F36V

    Plasmid
    #187959
    Purpose
    Expressed ABA-inducible dimerizing KRAB-dCas9 system, with KRAB-IRES-Blasticidin resistance under CAG promoter and tagBFP-dCas9-FKBP12 (F36V mutant) degron under PGK promoter in a piggyBac plasmid.
    Depositor
    Inserts
    Blasticidin resistance
    FKBP12_F36V
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  32. pSBBi-GB CaXMT1

    Plasmid
    #139168
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica xanthosine methyltransferase 1 (CaXMT1); the other side contains GFP and blasticidin resistance genes.
    Depositor
    Insert
    7-methylxanthosine synthase 1 [Coffea arabica]
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
  33. pSBBi-GB GST-CaXMT1

    Plasmid
    #139169
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains N-GST Coffea arabica xanthosine methyltransferase 1 (CaXMT1); the other side contains GFP and blasticidin resistance genes.
    Depositor
    Insert
    7-methylxanthosine synthase 1 [Coffea arabica]
    Use
    Transposon
    Tags
    glutathione transferase
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
  34. pSBBi-GB TCS2

    Plasmid
    #139171
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains tea caffeine synthase 1 (TCS2) from Camellia sinensis; the other side contains GFP and blasticidin resistance genes.
    Depositor
    Insert
    probable caffeine synthase 2 [Camellia sinensis]
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
  35. pMVP-SEC24C-G220C

    Plasmid
    #231560
    Purpose
    pMVP expression vector for human SEC24C (G220C mutation, closed, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)
    Depositor
    Insert
    SEC24C (G220C) (SEC24C Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    G220C, silent PAM mutations (G67 (GGG>GGA) and…
    Promoter
    CMV
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  36. pMVP-SEC24C

    Plasmid
    #231559
    Purpose
    pMVP expression vector for human SEC24C (wild type, closed, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)
    Depositor
    Insert
    SEC24C (SEC24C Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    silent PAM mutations (G67 (GGG>GGA) and A115 (…
    Promoter
    CMV
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 50 of 464 results