A versatile and efficient high-throughput cloning tool for structural biology.

Geertsma ER, Dutzler R
Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
49033	pTLNXA7	FX cloning Xenopus oocyte expression vector with SP6 promoter and N-terminal chicken alpha7 signal sequence
49032	pTLNX	FX cloning Xenopus oocyte expression vector with SP6 promoter
49031	pcDXC3GMS	FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, EGFP, Myc tag and streptavidin binding peptide
49030	pcDXC3MS	FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, Myc tag and streptavidin binding peptide
49029	pcDXNSMG3	FX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag, EGFP and 3C protease cleavage site
49028	pcDXNSM3	FX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag and 3C protease cleavage site
49027	pYEXC3GH	FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag
49026	pYEXC3H	FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site and 10x His tag
49025	pYEXNHG3	FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site
49024	pYEXNH3	FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage site