pICXC3GH
(Plasmid
#49023)
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Purpose(Empty Backbone) FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49023 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPICZ
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Backbone manufacturerInvitrogen
- Backbone size (bp) 5778
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Modifications to backbonemodified for compatibility with FX cloning and added tags/fusion proteins
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Vector typeYeast Expression
- Promoter AOX1
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Selectable markersZeocin
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Tags
/ Fusion Proteins
- 3C protease site (PreScission site) (C terminal on backbone)
- GFP (C terminal on backbone)
- decaHIS (10x His) (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Bleocin (Zeocin), 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Copy numberLow Copy
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer AOX1-fwd (5'-GACTGGTTCCAATTGACAAGC-3')
- 3′ sequencing primer AOX1-rev (5'-GCAAATGGCATTCTGACATCC-3') (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pICXC3GH was a gift from Raimund Dutzler & Eric Geertsma (Addgene plasmid # 49023 ; http://n2t.net/addgene:49023 ; RRID:Addgene_49023) -
For your References section:
A versatile and efficient high-throughput cloning tool for structural biology. Geertsma ER, Dutzler R. Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25. 10.1021/bi200178z PubMed 21410291