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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 35194 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepURA
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePCP-2x-yeGFP
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Alt name2X yeast enhanced green fluorescent protein
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SpeciesSynthetic
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid expresses a chimeric RNA-binding protein fused to GFP used to detect mRNAs that contain multiple copies of an RNA sequence (see Addgene plasmid #'s 35191, 35192 & 35196). See associated publication for detailed methods and use of this plasmid.
Please note that Addgene's sequencing results identified discrepancies when compared to the assembled sequence provided by the depositing laboratory. The first GFP construct in this plasmid (bp#441-1154) is yeast codon optimized and contains a E124K mutation and amino acid 206 is A (not K, as suggested by the depositor's sequence). The second GFP construct in this plasmid (bp# 1212-1928) is NOT yeast codon optimized.
This construct has successfully been used by the depositing laboratory as detailed in the associated publication and the sequencing discrepancies are not a concern for the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDZ276 (pURA MET28 PCP-2x-yeGFP) was a gift from Robert Singer (Addgene plasmid # 35194 ; http://n2t.net/addgene:35194 ; RRID:Addgene_35194) -
For your References section:
Real-time observation of transcription initiation and elongation on an endogenous yeast gene. Larson DR, Zenklusen D, Wu B, Chao JA, Singer RH. Science. 2011 Apr 22;332(6028):475-8. 10.1126/science.1202142 PubMed 21512033