SAFB associates with nascent RNAs and can promote gene expression in mouse embryonic stem cells.
Cherney R, Eberhard Q, Giri G, Mills C, Porrello A, Zhang Z, White D, Trotman JB, Herring L, Dominguez D, Calabrese M
RNA. 2023 Jul 19:rna.079569.122. doi: 10.1261/rna.079569.122.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196087 | pb-miRE-tre-3xFLAG-hnRNPU | Expresses mouse hnRNPU with a 3xFLAG tag in mammalian cells |
| 196088 | pb-miRE-tre-3xFLAG-hnRNPU∆RGG | Expresses mouse hnRNPU lacking RGG domain with a 3xFLAG tag in mammalian cells |
| 196089 | CAGGS-SAFB-NLS-3XFLAG-V5 | Expresses mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag |
| 196090 | CAGGS-EGFP-NLS-3XFLAG-V5 | Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag |
| 196091 | CAGGS-safb∆sap-NLS-3XFLAG-V5 | Expresses mouse SAFB lacking the n-terminal SAP domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag |
| 196092 | CAGGS-safb∆dd1-NLS-3XFLAG-V5 | Expresses mouse SAFB lacking the first computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag |
| 196093 | CAGGS-safb∆rrm-NLS-3XFLAG-V5 | Expresses mouse SAFB lacking the rrm domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag |
| 196094 | CAGGS-safb∆dd3-NLS-3XFLAG-V5 | Expresses mouse SAFB lacking the third computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag |
| 196095 | CAGGS-SAFB-DD3-NLS-3XFLAG-V5 | Expresses only the third computationally called disordered domain of mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag |
| 196103 | pX330_SAFBgRNA8 | Contains guide RNA to 3' end of mouse SAFB gene for safb1/2 dko. Used with Addgene IDs: 196106, 196107, 196108 |
| 196106 | pX330_SAFB2gRNA12 | Contains guide RNA to 3' end of mouse SAFB2 gene for safb1/2 dko. Used with Addgene IDs: 196103, 196107, 196108 |
| 196107 | pX330_SAFB2gRNA13 | Contains guide RNA to 3' end of mouse SAFB2 gene for safb1/2 dko. Used with Addgene IDs: 196103, 196106, 196108 |
| 196108 | pX330_SAFBgRNA9 | Contains guide RNA to 3' end of mouse SAFB gene for safb1/2 dko. Used with Addgene IDs: 196103, 196106, 196107 |
| 196383 | CAGGS-MCS-NLS-3XFLAG-V5 | Empty backbone vector to insert cDNA for strong expression in mammalian cells |