Mapping Interaction Sites on Human Chemokine Receptors by Deep Mutational Scanning.
Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E
J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 98942 | pcDNA3-CXCR4 | Mammalian expression plasmid for untagged human CXCR4 |
| 98943 | pcDNA3-CCR5 | Mammalian expression plasmid for untagged human CCR5 |
| 98944 | pCEP4-CXCR4 | Mammalian expression plasmid for untagged human CXCR4 |
| 98945 | pCEP4-CCR5 | Mammalian expression plasmid for untagged human CCR5 |
| 98946 | pCEP4-myc-CXCR4 | Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 |
| 98947 | pCEP4-FLAG-CXCR4 | Mammalian expression plasmid for N-terminal FLAG-tagged human CXCR4 |
| 98948 | pCEP4-myc-CCR5 | Mammalian expression plasmid for N-terminal myc-tagged human CCR5 |
| 98949 | pCEP4-FLAG-CCR5 | Mammalian expression plasmid for N-terminal FLAG-tagged human CCR5 |
| 98950 | pCEP4-HA-CCR5 | Mammalian expression plasmid for N-terminal HA-tagged human CCR5 |
| 98951 | pCEP4-myc-CXCR4-W161Y | Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant W161Y |
| 98952 | pCEP4-myc-CXCR4-W161H | Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant W161H |
| 98953 | pCEP4-myc-CXCR4-H79Q | Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant H79Q |
| 98954 | pCEP4-CCR5(R5gp120-Hi) | Mammalian expression plasmid for myc-tagged human CCR5 mutant R5gp120-Hi |
| 98957 | pCEP4-gp120(BaL)-CD4 | Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate |
| 98961 | pcDNA3-CXCL12-sfGFP | Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12 |
| 98962 | gRNA-CXCR4(90) | Mammalian expression plasmid for CRISPR gRNA targeting human CXCR4 exon 2 |
| 98963 | pCEP4-FLAG-CCR5-VN | FLAG-tagged human CCR5 fused to N-terminus of split Venus |
| 98964 | pCEP4-FLAG-CXCR4-VN | FLAG-tagged human CXCR4 fused to N-terminus of split Venus |
| 98965 | pCEP4-FLAG-GRM3-VN | FLAG-tagged human mGluR3 fused to N-terminus of split Venus |
| 98966 | pCEP4-myc-CCR5-VC | myc-tagged human CCR5 fused to C-terminus of split Venus |
| 98967 | pCEP4-myc-CXCR4-VC | myc-tagged human CXCR4 fused to C-terminus of split Venus |
| 98968 | pCEP4-myc-GRM3-VC | myc-tagged human mGluR3 fused to C-terminus of split Venus |