gRNA-CXCR4(90)
(Plasmid
#98962)
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PurposeMammalian expression plasmid for CRISPR gRNA targeting human CXCR4 exon 2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 98962 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR-Blunt II-TOPO
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Backbone manufacturerInvitrogen
- Total vector size (bp) 3974
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Modifications to backboneInsert was cloned into the gRNA_Cloning Vector (Addgene Plasmid #41824) from the George Church laboratory.
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCXCR4
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Alt nameCD184
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SpeciesH. sapiens (human)
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MutationEncodes a gRNA for CRISPR-mediated targeting of CXCR4.
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GenBank IDNM_003467
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Entrez GeneCXCR4 (a.k.a. CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS, WHIMS1)
- Promoter U6
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
gRNA-CXCR4(90) was a gift from Erik Procko (Addgene plasmid # 98962 ; http://n2t.net/addgene:98962 ; RRID:Addgene_98962) -
For your References section:
Mapping Interaction Sites on Human Chemokine Receptors by Deep Mutational Scanning. Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E. J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343. 10.4049/jimmunol.1800343 PubMed 29678950