pCEP4-myc-CXCR4-W161H
(Plasmid
#98952)
-
PurposeMammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant W161H
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 98952 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCEP4
-
Backbone manufacturerInvitrogen
-
Vector typeMammalian Expression
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCXCR4
-
Alt nameCD184
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1161
-
MutationW161H: enhanced CXCL12 binding and signaling
-
GenBank IDNM_003467
-
Entrez GeneCXCR4 (a.k.a. CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS, WHIMS1)
- Promoter CMV
-
Tags
/ Fusion Proteins
- Signal/leader sequence from Influenza A virus HA protein (N terminal on insert)
- c-myc epitope tag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV_fwd
- 3′ sequencing primer EBV_rev (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCEP4-myc-CXCR4-W161H was a gift from Erik Procko (Addgene plasmid # 98952 ; http://n2t.net/addgene:98952 ; RRID:Addgene_98952) -
For your References section:
Mapping Interaction Sites on Human Chemokine Receptors by Deep Mutational Scanning. Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E. J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343. 10.4049/jimmunol.1800343 PubMed 29678950