pSV Sport RXR alpha
(Plasmid #8882)

• Depositing Lab: Bruce Spiegelman
• Publication: Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

Backbone

• Vector backbone: pSV-Sport
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3160
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RXR alpha
• Alt name: RXRa
• Species: H. sapiens (human)
• Insert Size (bp): 1866
• Entrez Gene: RXRA (a.k.a. NR2B1, RXR-alpha, RXRalpha)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: cDNA for hRXRa from Dr. Ron Evans (Mangelsdorf et al. 1990 Nature 345 p224)
• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry