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Mouse Subpooled CRISPRi v2 Libraries
(Pooled Libraries #83989, #83990, #83991, #83992, #83993, #83994, #83995)

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  • Purpose

    Version 2 - Mouse subpooled CRISPRi libraries for inhibition of gene transcription. Available as five gRNAs per gene.

  • Vector Backbone

    pCRISPRia-v2 (Plasmid #84832) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 83989

  • Kinases, Phosphatases, and Drug Targets - gRNA pooled library†

1 $430 Add to Cart
Pooled Library 83990

  • Cancer and Apoptosis - gRNA pooled library†

1 $325 Add to Cart
Pooled Library 83991

  • Stress and Proteostasis - gRNA pooled library†

1 $325 Add to Cart
Pooled Library 83992

  • Mitochondria, Trafficking, and Motility - gRNA pooled library†

1 $325 Add to Cart
Pooled Library 83993

  • Gene expression - gRNA pooled library†

1 $325 Add to Cart
Pooled Library 83994

  • Membrane Proteins - gRNA pooled library†

1 $430 Add to Cart
Pooled Library 83995

  • Unassigned - gRNA pooled library†

1 $325 Add to Cart
Available to academic and nonprofits only.

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with dCas9-KRAB (#46911) or otherwise used with cell lines already expressing inhibitory dCas9.

  • If you would like to receive all seven sublibraries in a single order, please email [email protected] for pricing (if you use a distributor in your country, please contact your distributor directly for pricing).

Library Details

Pooled Library Genes Targeted Targeting gRNAs Control gRNAs
83989 2,269 12,100 250
83990 2,857 15,200 280
83991 2,800 14,685 290
83992 2,099 11,040 250
83993 1,917 10,090 250
83994 2,110 11,010 250
83995 5,951 31,120 600
  • Species
    Mouse
  • Lentiviral Generation
    3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    12.5 ng/µL

Resource Information

Depositor Comments

In addition to dCas9-KRAB (#46911), another dCas9-containing construct that can be used with these libraries is pHR-SFFV-KRAB-dCas9-P2A-mCherry (#60954).

The data analysis pipeline for analyzing sequencing data is available on GitHub.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCRISPRi v2 subpooled library was a gift from Jonathan Weissman (Addgene ID #83989)
    mCRISPRi v2 subpooled library was a gift from Jonathan Weissman (Addgene ID #83990)
    mCRISPRi v2 subpooled library was a gift from Jonathan Weissman (Addgene ID #83991)
    mCRISPRi v2 subpooled library was a gift from Jonathan Weissman (Addgene ID #83992)
    mCRISPRi v2 subpooled library was a gift from Jonathan Weissman (Addgene ID #83993)
    mCRISPRi v2 subpooled library was a gift from Jonathan Weissman (Addgene ID #83994)
    mCRISPRi v2 subpooled library was a gift from Jonathan Weissman (Addgene ID #83995)

  • For your References section:

    Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation . Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS Elife. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760. PubMed 27661255
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