Human Subpooled CRISPRi-v2 Libraries
(Pooled Libraries #83971, #83972, #83973, #83974, #83975, #83976, #83977)
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Purpose
Version 2 - Human subpooled CRISPRi libraries for inhibition of gene transcription. Available as 5 gRNAs per gene.
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Vector Backbone
pCRISPRia-v2 (Plasmid #84832) - does not express Cas9
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Depositing Labs
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |||
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Pooled Library | 83971 | Kinases, Phosphatases, and Drug Targets - gRNA pooled library† | 1 | $430 | Add to Cart | ||
Pooled Library | 83972 | Cancer and Apoptosis - gRNA pooled library† | 1 | $430 | Add to Cart | ||
Pooled Library | 83973 | Stress and Proteostasis - gRNA pooled library† | 1 | $430 | Add to Cart | ||
Pooled Library | 83974 | Mitochondria, Trafficking, and Motility - gRNA pooled library† | 1 | $430 | Add to Cart | ||
Pooled Library | 83975 | Gene expression - gRNA pooled library† | 1 | $430 | Add to Cart | ||
Pooled Library | 83976 | Membrane Proteins - gRNA pooled library† | 1 | $430 | Add to Cart | ||
Pooled Library | 83977 | Unassigned - gRNA pooled library† | 1 | $325 | Add to Cart |
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with dCas9-KRAB (#46911) or otherwise used with cell lines already expressing inhibitory dCas9.
If you would like to receive all seven sublibraries in a single order, please email [email protected] for pricing (if you use a distributor in your country, please contact your distributor directly for pricing).
Library Details
Pooled Library | Genes Targeted | Targeting gRNAs | Control gRNAs |
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83971 | 2,318 | 12,775 | 250 |
83972 | 2,916 | 16,045 | 280 |
83973 | 3,093 | 16,615 | 290 |
83974 | 2,219 | 12,050 | 250 |
83975 | 2,292 | 12,465 | 250 |
83976 | 2,418 | 12,955 | 250 |
83977 | 3,649 | 19,735 | 325 |
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SpeciesHuman
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Lentiviral Generation3rd
Library Shipping
Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.
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Volume∼20 µL
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Concentration12.5 ng/µL
Resource Information
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Protocols
- Illumina Sequencing Sample Preparation (PDF, 752 KB)
- Liquid Culture Re-Amplification Protocol (PDF, 71 KB)
- Cell Line Creation and Validation Primer (PDF, 125 KB)
- CRISPRi/a Custom Library Cloning Protocol (PDF, 92 KB)
- For more links and protocols, please see the Weismann lab's CRISPRi/a screening materials page.
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Depositor Data
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Terms and Licenses
Academic/Nonprofit TermsIndustry Terms- Not Available to Industry
Trademarks- Zeocin® is an InvivoGen trademark.
Depositor Comments
In addition to dCas9-KRAB (#46911), another dCas9-containing construct that can be used with these libraries is pHR-SFFV-KRAB-dCas9-P2A-mCherry (#60954).
The data analysis pipeline for analyzing sequencing data is available on GitHub.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hCRISPRi-v2 subpooled library was a gift from Jonathan Weissman (Addgene #83971)
hCRISPRi-v2 subpooled library was a gift from Jonathan Weissman (Addgene #83972)
hCRISPRi-v2 subpooled library was a gift from Jonathan Weissman (Addgene #83973)
hCRISPRi-v2 subpooled library was a gift from Jonathan Weissman (Addgene #83974)
hCRISPRi-v2 subpooled library was a gift from Jonathan Weissman (Addgene #83975)
hCRISPRi-v2 subpooled library was a gift from Jonathan Weissman (Addgene #83976)
hCRISPRi-v2 subpooled library was a gift from Jonathan Weissman (Addgene #83977) -
For your References section:
Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation. Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS Elife. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760. PubMed 27661255