pAAV-CAG-FLuc
(Plasmid #83281)

• Purpose: recombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)
• Depositing Lab: Mark Kay
• Publication: Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131610. doi: 10.1172/jci.insight.131610.

Backbone

• Vector backbone: pAAV with CAG promoter
• Backbone size w/o insert (bp): 4721
• Total vector size (bp): 6374
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Need to check for integrity of ITRs by restriction digest with AhdI, also with XmaI.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Firefly Luciferase
• Species: Photinus pyralis (Firefly)
• Insert Size (bp): 1653
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: GTTATTGTGCTGTCTCATC
• 3′ sequencing primer: CATTAAAGCAGCGTATCC

Resource Information

• Supplemental Documents:
  • pAAV-CAG-FLuc
• A portion of this plasmid was derived from a plasmid made by: AAV backbone as well as CAG promoter and WPRE sequences were obtained from Addgene plasmid #37825, sequences for the FLuc gene were obtained from Addgene plasmid #21375
• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry