LOC8R-PAGFP
(Plasmid
#66898)
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PurposePAGFP targeted to mitochondria using the synthetic LOC8R presequence
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66898 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPSmOrange-N1
- Backbone size w/o insert (bp) 3956
- Total vector size (bp) 5075
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLOC8R-PAGFP
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Alt nameMitochondrially targeted photoactivatable green fluorescent protein
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SpeciesAequorea victoria
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Insert Size (bp)1128
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Tag
/ Fusion Protein
- LOC8R (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The LOC8R targeting sequence was designed by Benjamin S. Padman during his doctoral research project (Primary supervisor: Georg Ramm)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LOC8R-PAGFP was a gift from Georg Ramm (Addgene plasmid # 66898 ; http://n2t.net/addgene:66898 ; RRID:Addgene_66898)
Map uploaded by the depositor.
