pCAT-YFP-TetR
(Plasmid #59018)

• Purpose: Expresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii
• Depositing Lab: David Sibley
• Publication: Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537-50. doi: 10.1016/j.chom.2014.04.002.

Backbone

• Vector backbone: pCAT-GFP
• Backbone manufacturer: Boris Striepen
• Backbone size w/o insert (bp): 6000
• Total vector size (bp): 7500
• Modifications to backbone: unknown
• Vector type: Toxoplasma Expression
• Selectable markers: chloramphenicol

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: YFP-TetR
• Alt name: Tetracycline Repressor Fused to Yellow Fluorescent Protein
• Species: Toxoplasma gondii
• Insert Size (bp): 1500
• Promoter: alpha-tubulin

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: avrII (unknown if destroyed)
• 3′ cloning site: pstI (unknown if destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry