pUPRT::DHFR-D
(Plasmid #58528)

• Purpose: replace TgUPRT with DHFR by homologous recombination
• Depositing Lab: David Sibley
• Publication: Shen et al MBio. 2014 May 13;5(3). pii: e01114-14. doi: 10.1128/mBio.01114-14.

Backbone

• Vector backbone: pDEST R4-R3 Vector II
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4555
• Total vector size (bp): 7635
• Vector type: Gateway cloning

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DHFR cassette
• Species: T. gondii
• Insert Size (bp): 3162
• Mutation: pyrimethamine resistant
• Promoter: DHFR

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 reverse
• 3′ sequencing primer: M13 forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pDHFR-TS as a template for DHFR cassette. from David Roos, Donald, R. G., and D. S. Roos. 1993. Stable molecular transformation of Toxoplasma gondii: a selectable dihydrofolate reductase-thymidylate synthase marker based on drug-resistance mutations in malaria. Proc Natl Acad Sci U S A 90:11703-7.
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry