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Addgene

pdCas9::BFP-humanized
(Plasmid #44247)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44247 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCVpuro
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression, Retroviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9 fused to BFP
  • Alt name
    Catalytically inactive Cas9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4881
  • Mutation
    D10A H840A (catalytically inactive)
  • Promoter MSCV LTR promoter
  • Tag / Fusion Protein
    • 2xNLS & tagBFP (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CTAGGCGCCGGAATTAGATCTCGCCACCATGGA
  • 3′ sequencing primer TCCCTCATTAAGCTTGTGCCCCAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pdCas9::BFP-humanized was a gift from Stanley Qi (Addgene plasmid # 44247 ; http://n2t.net/addgene:44247 ; RRID:Addgene_44247)

  • For your References section:

    Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. 10.1016/j.cell.2013.02.022 PubMed 23452860
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