hCas9 Citations (415)
Originally described in: RNA-Guided Human Genome Engineering via Cas9.Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM Science. 2013 Feb 15;339(6121):823-6. doi: 10.1126/science.1232033 PubMed Journal
Articles Citing hCas9
| Articles |
|---|
Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas.
Farzadfard F, Perli SD, Lu TK.
ACS Synth Biol. 2013 Sep 11.
PubMed
Associated Plasmids |
Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease.
Fujii W, Kawasaki K, Sugiura K, Naito K.
Nucleic Acids Res. 2013 Aug 30.
PubMed
Associated Plasmids |
| A simplified and efficient germline-specific CRISPR/Cas9 system for Drosophila genomic engineering. Sebo ZL, Lee HB, Peng Y, Guo Y. Fly (Austin). 2014;8(1):52-7. doi: 10.4161/fly.26828. Epub 2013 Oct 18. PubMed |
| CRISPR/Cas9-mediated targeted mutagenesis in the liverwort Marchantia polymorpha L. Sugano SS, Shirakawa M, Takagi J, Matsuda Y, Shimada T, Hara-Nishimura I, Kohchi T. Plant Cell Physiol. 2014 Mar;55(3):475-81. doi: 10.1093/pcp/pcu014. Epub 2014 Jan 18. PubMed |
| CRISPR-Cas-mediated targeted genome editing in human cells. Yang L, Mali P, Kim-Kiselak C, Church G. Methods Mol Biol. 2014;1114:245-67. doi: 10.1007/978-1-62703-761-7_16. PubMed |
| Role of mouse and human autophagy proteins in IFN-gamma-induced cell-autonomous responses against Toxoplasma gondii. Ohshima J, Lee Y, Sasai M, Saitoh T, Su Ma J, Kamiyama N, Matsuura Y, Pann-Ghill S, Hayashi M, Ebisu S, Takeda K, Akira S, Yamamoto M. J Immunol. 2014 Apr 1;192(7):3328-35. doi: 10.4049/jimmunol.1302822. Epub 2014 Feb 21. PubMed |
| High-precision robotic microcontact printing (R-muCP) utilizing a vision guided selectively compliant articulated robotic arm. McNulty JD, Klann T, Sha J, Salick M, Knight GT, Turng LS, Ashton RS. Lab Chip. 2014 Jun 7;14(11):1923-30. doi: 10.1039/c3lc51137e. Epub 2014 Apr 24. PubMed |
| A high-coverage shRNA screen identifies TMEM129 as an E3 ligase involved in ER-associated protein degradation. van de Weijer ML, Bassik MC, Luteijn RD, Voorburg CM, Lohuis MA, Kremmer E, Hoeben RC, LeProust EM, Chen S, Hoelen H, Ressing ME, Patena W, Weissman JS, McManus MT, Wiertz EJ, Lebbink RJ. Nat Commun. 2014 May 8;5:3832. doi: 10.1038/ncomms4832. PubMed |
Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells.
Maggio I, Holkers M, Liu J, Janssen JM, Chen X, Goncalves MA.
Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105.
PubMed
Associated Plasmids |
| Engineering human tumour-associated chromosomal translocations with the RNA-guided CRISPR-Cas9 system. Torres R, Martin MC, Garcia A, Cigudosa JC, Ramirez JC, Rodriguez-Perales S. Nat Commun. 2014 Jun 3;5:3964. doi: 10.1038/ncomms4964. PubMed |
| Biallelic targeting of expressed genes in mouse embryonic stem cells using the Cas9 system. Zhang Y, Vanoli F, LaRocque JR, Krawczyk PM, Jasin M. Methods. 2014 Sep;69(2):171-8. doi: 10.1016/j.ymeth.2014.05.003. Epub 2014 Jun 12. PubMed |
| Rapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system. Inui M, Miyado M, Igarashi M, Tamano M, Kubo A, Yamashita S, Asahara H, Fukami M, Takada S. Sci Rep. 2014 Jun 23;4:5396. doi: 10.1038/srep05396. PubMed |
Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform.
Yoshimi K, Kaneko T, Voigt B, Mashimo T.
Nat Commun. 2014 Jun 26;5:4240. doi: 10.1038/ncomms5240.
PubMed
Associated Plasmids |
| CRISPR/Cas9-Directed Genome Editing of Cultured Cells. Yang L, Yang JL, Byrne S, Pan J, Church GM. Curr Protoc Mol Biol. 2014 Jul 1;107:31.1.1-31.1.17. doi: 10.1002/0471142727.mb3101s107. PubMed |
| Whole-genome sequencing analysis reveals high specificity of CRISPR/Cas9 and TALEN-based genome editing in human iPSCs. Smith C, Gore A, Yan W, Abalde-Atristain L, Li Z, He C, Wang Y, Brodsky RA, Zhang K, Cheng L, Ye Z. Cell Stem Cell. 2014 Jul 3;15(1):12-3. doi: 10.1016/j.stem.2014.06.011. PubMed |
| A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice. Sakurai T, Watanabe S, Kamiyoshi A, Sato M, Shindo T. BMC Biotechnol. 2014 Jul 21;14:69. doi: 10.1186/1472-6750-14-69. PubMed |
| PVT1 dependence in cancer with MYC copy-number increase. Tseng YY, Moriarity BS, Gong W, Akiyama R, Tiwari A, Kawakami H, Ronning P, Reuland B, Guenther K, Beadnell TC, Essig J, Otto GM, O'Sullivan MG, Largaespada DA, Schwertfeger KL, Marahrens Y, Kawakami Y, Bagchi A. Nature. 2014 Aug 7;512(7512):82-6. doi: 10.1038/nature13311. Epub 2014 Jun 22. PubMed |
| In vitro and in vivo growth suppression of human papillomavirus 16-positive cervical cancer cells by CRISPR/Cas9. Zhen S, Hua L, Takahashi Y, Narita S, Liu YH, Li Y. Biochem Biophys Res Commun. 2014 Aug 8;450(4):1422-6. doi: 10.1016/j.bbrc.2014.07.014. Epub 2014 Jul 17. PubMed |
| Using RNA-seq and targeted nucleases to identify mechanisms of drug resistance in acute myeloid leukemia. Rathe SK, Moriarity BS, Stoltenberg CB, Kurata M, Aumann NK, Rahrmann EP, Bailey NJ, Melrose EG, Beckmann DA, Liska CR, Largaespada DA. Sci Rep. 2014 Aug 13;4:6048. doi: 10.1038/srep06048. PubMed |
Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector.
Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA.
Nucleic Acids Res. 2014 Aug 13. pii: gku749.
PubMed
Associated Plasmids |
| Prevention of muscular dystrophy in mice by CRISPR/Cas9-mediated editing of germline DNA. Long C, McAnally JR, Shelton JM, Mireault AA, Bassel-Duby R, Olson EN. Science. 2014 Sep 5;345(6201):1184-1188. doi: 10.1126/science.1254445. Epub 2014 Aug 14. PubMed |
| The CRISPR/Cas9 System Facilitates Clearance of the Intrahepatic HBV Templates In Vivo. Lin SR, Yang HC, Kuo YT, Liu CJ, Yang TY, Sung KC, Lin YY, Wang HY, Wang CC, Shen YC, Wu FY, Kao JH, Chen DS, Chen PJ. Mol Ther Nucleic Acids. 2014 Aug 19;3:e186. doi: 10.1038/mtna.2014.38. PubMed |
| Adenoviral vector DNA for accurate genome editing with engineered nucleases. Holkers M, Maggio I, Henriques SF, Janssen JM, Cathomen T, Goncalves MA. Nat Methods. 2014 Oct;11(10):1051-7. doi: 10.1038/nmeth.3075. Epub 2014 Aug 24. PubMed |
| Nascent RNA interaction keeps PRC2 activity poised and in check. Kaneko S, Son J, Bonasio R, Shen SS, Reinberg D. Genes Dev. 2014 Sep 15;28(18):1983-8. doi: 10.1101/gad.247940.114. Epub 2014 Aug 28. PubMed |
Improving the specificity and efficacy of CRISPR/CAS9 and gRNA through target specific DNA reporter.
Zhang JH, Pandey M, Kahler JF, Loshakov A, Harris B, Dagur PK, Mo Y, Simonds WF.
J Biotechnol. 2014 Sep 2. pii: S0168-1656(14)00838-4. doi: 10.1016/j.jbiotec.2014.08.033.
PubMed
Associated Plasmids |
Precise gene deletion and replacement using the CRISPR/Cas9 system in human cells.
Zheng Q, Cai X, Tan MH, Schaffert S, Arnold CP, Gong X, Chen CZ, Huang S.
Biotechniques. 2014 Sep 1;57(3):115-24. doi: 10.2144/000114196. eCollection 2014.
PubMed
Associated Plasmids |
| Simple knockout by electroporation of engineered endonucleases into intact rat embryos. Kaneko T, Sakuma T, Yamamoto T, Mashimo T. Sci Rep. 2014 Oct 1;4:6382. doi: 10.1038/srep06382. PubMed |
Mouse Genome Editing Using the CRISPR/Cas System.
Harms DW, Quadros RM, Seruggia D, Ohtsuka M, Takahashi G, Montoliu L, Gurumurthy CB.
Curr Protoc Hum Genet. 2014 Oct 1;83:15.7.1-15.7.27. doi: 10.1002/0471142905.hg1507s83.
PubMed
Associated Plasmids |
| Generation of CRISPR/Cas9-mediated gene-targeted pigs via somatic cell nuclear transfer. Zhou X, Xin J, Fan N, Zou Q, Huang J, Ouyang Z, Zhao Y, Zhao B, Liu Z, Lai S, Yi X, Guo L, Esteban MA, Zeng Y, Yang H, Lai L. Cell Mol Life Sci. 2015 Mar;72(6):1175-84. doi: 10.1007/s00018-014-1744-7. Epub 2014 Oct 2. PubMed |
| Improved genome editing in human cell lines using the CRISPR method. Munoz IM, Szyniarowski P, Toth R, Rouse J, Lachaud C. PLoS One. 2014 Oct 10;9(10):e109752. doi: 10.1371/journal.pone.0109752. eCollection 2014. PubMed |
| IFI16 restricts HSV-1 replication by accumulating on the hsv-1 genome, repressing HSV-1 gene expression, and directly or indirectly modulating histone modifications. Johnson KE, Bottero V, Flaherty S, Dutta S, Singh VV, Chandran B. PLoS Pathog. 2014 Nov 6;10(11):e1004503. doi: 10.1371/journal.ppat.1004503. eCollection 2014 Nov. PubMed |
| Off-target assessment of CRISPR-Cas9 guiding RNAs in human iPS and mouse ES cells. Tan EP, Li Y, Velasco-Herrera Mdel C, Yusa K, Bradley A. Genesis. 2015 Feb;53(2):225-36. doi: 10.1002/dvg.22835. Epub 2014 Dec 10. PubMed |
| Assaying break and nick-induced homologous recombination in mammalian cells using the DR-GFP reporter and Cas9 nucleases. Vriend LE, Jasin M, Krawczyk PM. Methods Enzymol. 2014;546:175-91. doi: 10.1016/B978-0-12-801185-0.00009-X. PubMed |
| Efficient and allele-specific genome editing of disease loci in human iPSCs. Smith C, Abalde-Atristain L, He C, Brodsky BR, Braunstein EM, Chaudhari P, Jang YY, Cheng L, Ye Z. Mol Ther. 2015 Mar;23(3):570-7. doi: 10.1038/mt.2014.226. Epub 2014 Nov 24. PubMed |
| Kinetic pathway of 40S ribosomal subunit recruitment to hepatitis C virus internal ribosome entry site. Fuchs G, Petrov AN, Marceau CD, Popov LM, Chen J, O'Leary SE, Wang R, Carette JE, Sarnow P, Puglisi JD. Proc Natl Acad Sci U S A. 2015 Jan 13;112(2):319-25. doi: 10.1073/pnas.1421328111. Epub 2014 Dec 16. PubMed |
| Fanconi anemia gene editing by the CRISPR/Cas9 system. Osborn MJ, Gabriel R, Webber BR, DeFeo AP, McElroy AN, Jarjour J, Starker CG, Wagner JE, Joung JK, Voytas DF, von Kalle C, Schmidt M, Blazar BR, Tolar J. Hum Gene Ther. 2015 Feb;26(2):114-26. doi: 10.1089/hum.2014.111. PubMed |
| Gene Targeting Through Homologous Recombination in Monkey Embryonic Stem Cells Using CRISPR/Cas9 System. Zhu S, Rong Z, Lu X, Xu Y, Fu X. Stem Cells Dev. 2015 May 15;24(10):1147-9. doi: 10.1089/scd.2014.0507. Epub 2015 Feb 25. PubMed |
| Harnessing the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated Cas9 system to disrupt the hepatitis B virus. Zhen S, Hua L, Liu YH, Gao LC, Fu J, Wan DY, Dong LH, Song HF, Gao X. Gene Ther. 2015 May;22(5):404-12. doi: 10.1038/gt.2015.2. Epub 2015 Feb 5. PubMed |
| Production of Gene-Corrected Adult Beta Globin Protein in Human Erythrocytes Differentiated from Patient iPSCs After Genome Editing of the Sickle Point Mutation. Huang X, Wang Y, Yan W, Smith C, Ye Z, Wang J, Gao Y, Mendelsohn L, Cheng L. Stem Cells. 2015 May;33(5):1470-9. doi: 10.1002/stem.1969. PubMed |
| A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture. O'Geen H, Henry IM, Bhakta MS, Meckler JF, Segal DJ. Nucleic Acids Res. 2015 Mar 31;43(6):3389-404. doi: 10.1093/nar/gkv137. Epub 2015 Feb 20. PubMed |
| CRISPR/Cas9 as tool for functional study of genes involved in preimplantation embryo development. Kwon J, Namgoong S, Kim NH. PLoS One. 2015 Mar 16;10(3):e0120501. doi: 10.1371/journal.pone.0120501. eCollection 2015. PubMed |
| Selection of a hepatitis C virus with altered entry factor requirements reveals a genetic interaction between the E1 glycoprotein and claudins. Hopcraft SE, Evans MJ. Hepatology. 2015 Mar 29. doi: 10.1002/hep.27815. PubMed |
| Interaction of the Clostridium difficile Binary Toxin CDT and Its Host Cell Receptor, Lipolysis-stimulated Lipoprotein Receptor (LSR). Hemmasi S, Czulkies BA, Schorch B, Veit A, Aktories K, Papatheodorou P. J Biol Chem. 2015 May 29;290(22):14031-44. doi: 10.1074/jbc.M115.650523. Epub 2015 Apr 16. PubMed |
| Functional validation of mouse tyrosinase non-coding regulatory DNA elements by CRISPR-Cas9-mediated mutagenesis. Seruggia D, Fernandez A, Cantero M, Pelczar P, Montoliu L. Nucleic Acids Res. 2015 May 26;43(10):4855-67. doi: 10.1093/nar/gkv375. Epub 2015 Apr 20. PubMed |
| Nanoparticles that deliver triplex-forming peptide nucleic acid molecules correct F508del CFTR in airway epithelium. McNeer NA, Anandalingam K, Fields RJ, Caputo C, Kopic S, Gupta A, Quijano E, Polikoff L, Kong Y, Bahal R, Geibel JP, Glazer PM, Saltzman WM, Egan ME. Nat Commun. 2015 Apr 27;6:6952. doi: 10.1038/ncomms7952. PubMed |
| Cas9-mediated targeting of viral RNA in eukaryotic cells. Price AA, Sampson TR, Ratner HK, Grakoui A, Weiss DS. Proc Natl Acad Sci U S A. 2015 May 12;112(19):6164-9. doi: 10.1073/pnas.1422340112. Epub 2015 Apr 27. PubMed |
| Sequential cancer mutations in cultured human intestinal stem cells. Drost J, van Jaarsveld RH, Ponsioen B, Zimberlin C, van Boxtel R, Buijs A, Sachs N, Overmeer RM, Offerhaus GJ, Begthel H, Korving J, van de Wetering M, Schwank G, Logtenberg M, Cuppen E, Snippert HJ, Medema JP, Kops GJ, Clevers H. Nature. 2015 May 7;521(7550):43-7. doi: 10.1038/nature14415. Epub 2015 Apr 29. PubMed |
| An alternative pluripotent state confers interspecies chimaeric competency. Wu J, Okamura D, Li M, Suzuki K, Luo C, Ma L, He Y, Li Z, Benner C, Tamura I, Krause MN, Nery JR, Du T, Zhang Z, Hishida T, Takahashi Y, Aizawa E, Kim NY, Lajara J, Guillen P, Campistol JM, Esteban CR, Ross PJ, Saghatelian A, Ren B, Ecker JR, Izpisua Belmonte JC. Nature. 2015 May 21;521(7552):316-21. doi: 10.1038/nature14413. Epub 2015 May 6. PubMed |
| The Nuclear DNA Sensor IFI16 Acts as a Restriction Factor for Human Papillomavirus Replication through Epigenetic Modifications of the Viral Promoters. Lo Cigno I, De Andrea M, Borgogna C, Albertini S, Landini MM, Peretti A, Johnson KE, Chandran B, Landolfo S, Gariglio M. J Virol. 2015 Aug 1;89(15):7506-20. doi: 10.1128/JVI.00013-15. Epub 2015 May 13. PubMed |
| ATRX represses alternative lengthening of telomeres. Napier CE, Huschtscha LI, Harvey A, Bower K, Noble JR, Hendrickson EA, Reddel RR. Oncotarget. 2015 Jun 30;6(18):16543-58. PubMed |
| Modulation of Gene Expression by Polymer Nanocapsule Delivery of DNA Cassettes Encoding Small RNAs. Yan M, Wen J, Liang M, Lu Y, Kamata M, Chen IS. PLoS One. 2015 Jun 2;10(6):e0127986. doi: 10.1371/journal.pone.0127986. eCollection 2015. PubMed |
| Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9. Hara S, Tamano M, Yamashita S, Kato T, Saito T, Sakuma T, Yamamoto T, Inui M, Takada S. Sci Rep. 2015 Jun 9;5:11221. doi: 10.1038/srep11221. PubMed |
| Molecular characterization of LC3-associated phagocytosis reveals distinct roles for Rubicon, NOX2 and autophagy proteins. Martinez J, Malireddi RK, Lu Q, Cunha LD, Pelletier S, Gingras S, Orchard R, Guan JL, Tan H, Peng J, Kanneganti TD, Virgin HW, Green DR. Nat Cell Biol. 2015 Jul;17(7):893-906. doi: 10.1038/ncb3192. Epub 2015 Jun 22. PubMed |
| Haploid Genetic Screen Reveals a Profound and Direct Dependence on Cholesterol for Hantavirus Membrane Fusion. Kleinfelter LM, Jangra RK, Jae LT, Herbert AS, Mittler E, Stiles KM, Wirchnianski AS, Kielian M, Brummelkamp TR, Dye JM, Chandran K. MBio. 2015 Jun 30;6(4):e00801. doi: 10.1128/mBio.00801-15. PubMed |
| CCAR2/DBC1 is required for Chk2-dependent KAP1 phosphorylation and repair of DNA damage. Magni M, Ruscica V, Restelli M, Fontanella E, Buscemi G, Zannini L. Oncotarget. 2015 Jul 10;6(19):17817-31. PubMed |
| MiT/TFE transcription factors are activated during mitophagy downstream of Parkin and Atg5. Nezich CL, Wang C, Fogel AI, Youle RJ. J Cell Biol. 2015 Aug 3;210(3):435-50. doi: 10.1083/jcb.201501002. PubMed |
| Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations. Sato M, Koriyama M, Watanabe S, Ohtsuka M, Sakurai T, Inada E, Saitoh I, Nakamura S, Miyoshi K. Int J Mol Sci. 2015 Aug 3;16(8):17838-56. doi: 10.3390/ijms160817838. PubMed |
| CRISPR mediated somatic cell genome engineering in the chicken. Veron N, Qu Z, Kipen PA, Hirst CE, Marcelle C. Dev Biol. 2015 Nov 1;407(1):68-74. doi: 10.1016/j.ydbio.2015.08.007. Epub 2015 Aug 13. PubMed |
| Cell-Cycle Control of Bivalent Epigenetic Domains Regulates the Exit from Pluripotency. Singh AM, Sun Y, Li L, Zhang W, Wu T, Zhao S, Qin Z, Dalton S. Stem Cell Reports. 2015 Sep 8;5(3):323-36. doi: 10.1016/j.stemcr.2015.07.005. Epub 2015 Aug 13. PubMed |
| Knockout of cGAS and STING Rescues Virus Infection of Plasmid DNA-Transfected Cells. Langereis MA, Rabouw HH, Holwerda M, Visser LJ, van Kuppeveld FJ. J Virol. 2015 Nov;89(21):11169-73. doi: 10.1128/JVI.01781-15. Epub 2015 Aug 26. PubMed |
Cas9 gRNA engineering for genome editing, activation and repression.
Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR, Collins JJ, Weiss R, Church G.
Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
PubMed
Associated Plasmids |
| Research of methods to detect genomic mutations induced by CRISPR/Cas systems. Wang K, Mei DY, Liu QN, Qiao XH, Ruan WM, Huang T, Cao GS. J Biotechnol. 2015 Nov 20;214:128-32. doi: 10.1016/j.jbiotec.2015.09.029. Epub 2015 Sep 28. PubMed |
| Use of the HPRT gene to study nuclease-induced DNA double-strand break repair. Gravells P, Ahrabi S, Vangala RK, Tomita K, Brash JT, Brustle LA, Chung C, Hong JM, Kaloudi A, Humphrey TC, Porter AC. Hum Mol Genet. 2015 Dec 15;24(24):7097-110. doi: 10.1093/hmg/ddv409. Epub 2015 Sep 30. PubMed |
| Monoclonal antibody profiling of cell surface proteins associated with the viral biofilms on HTLV-1 transformed cells. Tarasevich A, Filatov A, Pichugin A, Mazurov D. Acta Virol. 2015 Sep;59(3):247-56. PubMed |
| Homology-directed repair in rodent zygotes using Cas9 and TALEN engineered proteins. Menoret S, De Cian A, Tesson L, Remy S, Usal C, Boule JB, Boix C, Fontaniere S, Creneguy A, Nguyen TH, Brusselle L, Thinard R, Gauguier D, Concordet JP, Cherifi Y, Fraichard A, Giovannangeli C, Anegon I. Sci Rep. 2015 Oct 7;5:14410. doi: 10.1038/srep14410. PubMed |
| Disruption of MeCP2 attenuates circadian rhythm in CRISPR/Cas9-based Rett syndrome model mouse. Tsuchiya Y, Minami Y, Umemura Y, Watanabe H, Ono D, Nakamura W, Takahashi T, Honma S, Kondoh G, Matsuishi T, Yagita K. Genes Cells. 2015 Dec;20(12):992-1005. doi: 10.1111/gtc.12305. Epub 2015 Oct 12. PubMed |
| Glucocorticoid receptor antagonism reverts docetaxel resistance in human prostate cancer. Kroon J, Puhr M, Buijs JT, van der Horst G, Hemmer DM, Marijt KA, Hwang MS, Masood M, Grimm S, Storm G, Metselaar JM, Meijer OC, Culig Z, van der Pluijm G. Endocr Relat Cancer. 2016 Jan;23(1):35-45. doi: 10.1530/ERC-15-0343. Epub 2015 Oct 19. PubMed |
| Effect of Multiple Clock Gene Ablations on the Circadian Period Length and Temperature Compensation in Mammalian Cells. Tsuchiya Y, Umemura Y, Minami Y, Koike N, Hosokawa T, Hara M, Ito H, Inokawa H, Yagita K. J Biol Rhythms. 2016 Feb;31(1):48-56. doi: 10.1177/0748730415613888. Epub 2015 Oct 28. PubMed |
| Simple Genome Editing of Rodent Intact Embryos by Electroporation. Kaneko T, Mashimo T. PLoS One. 2015 Nov 10;10(11):e0142755. doi: 10.1371/journal.pone.0142755. eCollection 2015. PubMed |
| Differentiation of human ESCs to retinal ganglion cells using a CRISPR engineered reporter cell line. Sluch VM, Davis CH, Ranganathan V, Kerr JM, Krick K, Martin R, Berlinicke CA, Marsh-Armstrong N, Diamond JS, Mao HQ, Zack DJ. Sci Rep. 2015 Nov 13;5:16595. doi: 10.1038/srep16595. PubMed |
Simultaneous Reprogramming and Gene Correction of Patient Fibroblasts.
Howden SE, Maufort JP, Duffin BM, Elefanty AG, Stanley EG, Thomson JA.
Stem Cell Reports. 2015 Dec 8;5(6):1109-18. doi: 10.1016/j.stemcr.2015.10.009. Epub 2015 Nov 12.
PubMed
Associated Plasmids |
| Impaired PRC2 activity promotes transcriptional instability and favors breast tumorigenesis. Wassef M, Rodilla V, Teissandier A, Zeitouni B, Gruel N, Sadacca B, Irondelle M, Charruel M, Ducos B, Michaud A, Caron M, Marangoni E, Chavrier P, Le Tourneau C, Kamal M, Pasmant E, Vidaud M, Servant N, Reyal F, Meseure D, Vincent-Salomon A, Fre S, Margueron R. Genes Dev. 2015 Dec 15;29(24):2547-62. doi: 10.1101/gad.269522.115. Epub 2015 Dec 4. PubMed |
| Atg13 Is Essential for Autophagy and Cardiac Development in Mice. Kaizuka T, Mizushima N. Mol Cell Biol. 2015 Dec 7;36(4):585-95. doi: 10.1128/MCB.01005-15. PubMed |
| Genome editing through large insertion leads to the skipping of targeted exon. Uddin B, Chen NP, Panic M, Schiebel E. BMC Genomics. 2015 Dec 21;16(1):1082. doi: 10.1186/s12864-015-2284-8. PubMed |
| Filovirus receptor NPC1 contributes to species-specific patterns of ebolavirus susceptibility in bats. Ng M, Ndungo E, Kaczmarek ME, Herbert AS, Binger T, Kuehne AI, Jangra RK, Hawkins JA, Gifford RJ, Biswas R, Demogines A, James RM, Yu M, Brummelkamp TR, Drosten C, Wang LF, Kuhn JH, Muller MA, Dye JM, Sawyer SL, Chandran K. Elife. 2015 Dec 23;4. pii: e11785. doi: 10.7554/eLife.11785. PubMed |
| A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes. Nakao H, Harada T, Nakao K, Kiyonari H, Inoue K, Furuta Y, Aiba A. Genesis. 2015 Dec 29. doi: 10.1002/dvg.22914. PubMed |
| High content analysis platform for optimization of lipid mediated CRISPR-Cas9 delivery strategies in human cells. Steyer B, Carlson-Stevermer J, Angenent-Mari N, Khalil A, Harkness T, Saha K. Acta Biomater. 2015 Dec 30. pii: S1742-7061(15)30273-7. doi: 10.1016/j.actbio.2015.12.036. PubMed |
| Generation of Knock-In Pigs Carrying Oct4-tdTomato Reporter through CRISPR/Cas9-Mediated Genome Engineering. Lai S, Wei S, Zhao B, Ouyang Z, Zhang Q, Fan N, Liu Z, Zhao Y, Yan Q, Zhou X, Li L, Xin J, Zeng Y, Lai L, Zou Q. PLoS One. 2016 Jan 12;11(1):e0146562. doi: 10.1371/journal.pone.0146562. eCollection 2016. PubMed |
| Selection-free gene repair after adenoviral vector transduction of designer nucleases: rescue of dystrophin synthesis in DMD muscle cell populations. Maggio I, Stefanucci L, Janssen JM, Liu J, Chen X, Mouly V, Goncalves MA. Nucleic Acids Res. 2016 Jan 13. pii: gkv1540. PubMed |
| Golgi-Resident GTPase Rab30 Promotes the Biogenesis of Pathogen-Containing Autophagosomes. Oda S, Nozawa T, Nozawa-Minowa A, Tanaka M, Aikawa C, Harada H, Nakagawa I. PLoS One. 2016 Jan 15;11(1):e0147061. doi: 10.1371/journal.pone.0147061. eCollection 2016. PubMed |
| Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators. Wei S, Zou Q, Lai S, Zhang Q, Li L, Yan Q, Zhou X, Zhong H, Lai L. Sci Rep. 2016 Jan 19;6:19648. doi: 10.1038/srep19648. PubMed |
ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes.
Yoshimi K, Kunihiro Y, Kaneko T, Nagahora H, Voigt B, Mashimo T.
Nat Commun. 2016 Jan 20;7:10431. doi: 10.1038/ncomms10431.
PubMed
Associated Plasmids |
| Redundant function of DNA ligase 1 and 3 in alternative end-joining during immunoglobulin class switch recombination. Masani S, Han L, Meek K, Yu K. Proc Natl Acad Sci U S A. 2016 Feb 2;113(5):1261-6. doi: 10.1073/pnas.1521630113. Epub 2016 Jan 19. PubMed |
| Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining. Lu G, Duan J, Shu S, Wang X, Gao L, Guo J, Zhang Y. Proc Natl Acad Sci U S A. 2016 Feb 2;113(5):1256-60. doi: 10.1073/pnas.1521597113. Epub 2016 Jan 19. PubMed |
| Cancer-associated SF3B1 mutations affect alternative splicing by promoting alternative branchpoint usage. Alsafadi S, Houy A, Battistella A, Popova T, Wassef M, Henry E, Tirode F, Constantinou A, Piperno-Neumann S, Roman-Roman S, Dutertre M, Stern MH. Nat Commun. 2016 Feb 4;7:10615. doi: 10.1038/ncomms10615. PubMed |
A Vector with a Single Promoter for In Vitro Transcription and Mammalian Cell Expression of CRISPR gRNAs.
Romanienko PJ, Giacalone J, Ingenito J, Wang Y, Isaka M, Johnson T, You Y, Mark WH.
PLoS One. 2016 Feb 5;11(2):e0148362. doi: 10.1371/journal.pone.0148362. eCollection 2016.
PubMed
Associated Plasmids |
Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair.
He X, Tan C, Wang F, Wang Y, Zhou R, Cui D, You W, Zhao H, Ren J, Feng B.
Nucleic Acids Res. 2016 May 19;44(9):e85. doi: 10.1093/nar/gkw064. Epub 2016 Feb 4.
PubMed
Associated Plasmids |
| Direct Visualization of Ebola Virus Fusion Triggering in the Endocytic Pathway. Spence JS, Krause TB, Mittler E, Jangra RK, Chandran K. MBio. 2016 Feb 9;7(1):e01857-15. doi: 10.1128/mBio.01857-15. PubMed |
| Induction of site-specific chromosomal translocations in embryonic stem cells by CRISPR/Cas9. Jiang J, Zhang L, Zhou X, Chen X, Huang G, Li F, Wang R, Wu N, Yan Y, Tong C, Srivastava S, Wang Y, Liu H, Ying QL. Sci Rep. 2016 Feb 22;6:21918. doi: 10.1038/srep21918. PubMed |
| Regulation of Transcription Factor Yin Yang 1 by SET7/9-mediated Lysine Methylation. Zhang WJ, Wu XN, Shi TT, Xu HT, Yi J, Shen HF, Huang MF, Shu XY, Wang FF, Peng BL, Xiao RQ, Gao WW, Ding JC, Liu W. Sci Rep. 2016 Feb 23;6:21718. doi: 10.1038/srep21718. PubMed |
| T cell-specific inactivation of mouse CD2 by CRISPR/Cas9. Beil-Wagner J, Dossinger G, Schober K, vom Berg J, Tresch A, Grandl M, Palle P, Mair F, Gerhard M, Becher B, Busch DH, Buch T. Sci Rep. 2016 Feb 23;6:21377. doi: 10.1038/srep21377. PubMed |
| Transglutaminase 2 contributes to a TP53-induced autophagy program to prevent oncogenic transformation. Yeo SY, Itahana Y, Guo AK, Han R, Iwamoto K, Nguyen HT, Bao Y, Kleiber K, Wu YJ, Bay BH, Voorhoeve M, Itahana K. Elife. 2016 Mar 9;5:e07101. doi: 10.7554/eLife.07101. PubMed |
| Utilization of the CRISPR/Cas9 system for the efficient production of mutant mice using crRNA/tracrRNA with Cas9 nickase and FokI-dCas9. Terao M, Tamano M, Hara S, Kato T, Kinoshita M, Takada S. Exp Anim. 2016 Mar 14. PubMed |
| Distinct genetic control of homologous recombination repair of Cas9-induced double-strand breaks, nicks and paired nicks. Vriend LE, Prakash R, Chen CC, Vanoli F, Cavallo F, Zhang Y, Jasin M, Krawczyk PM. Nucleic Acids Res. 2016 Jun 20;44(11):5204-17. doi: 10.1093/nar/gkw179. Epub 2016 Mar 21. PubMed |
| A Traceless Selection: Counter-selection System That Allows Efficient Generation of Transposon and CRISPR-modified T-cell Products. Mezzadra R, Hollenstein A, Gomez-Eerland R, Schumacher TN. Mol Ther Nucleic Acids. 2016 Mar 22;5:e298. doi: 10.1038/mtna.2016.13. PubMed |
| Efficient genome editing of genes involved in neural crest development using the CRISPR/Cas9 system in Xenopus embryos. Liu Z, Cheng TT, Shi Z, Liu Z, Lei Y, Wang C, Shi W, Chen X, Qi X, Cai D, Feng B, Deng Y, Chen Y, Zhao H. Cell Biosci. 2016 Mar 31;6:22. doi: 10.1186/s13578-016-0088-4. eCollection 2016. PubMed |
| pEVL: A Linear Plasmid for Generating mRNA IVT Templates With Extended Encoded Poly(A) Sequences. Grier AE, Burleigh S, Sahni J, Clough CA, Cardot V, Choe DC, Krutein MC, Rawlings DJ, Jensen MC, Scharenberg AM, Jacoby K. Mol Ther Nucleic Acids. 2016 Apr 19;5:e306. doi: 10.1038/mtna.2016.21. PubMed |
| Multiplexed Targeted Genome Engineering Using a Universal Nuclease-Assisted Vector Integration System. Brown A, Woods WS, Perez-Pinera P. ACS Synth Biol. 2016 Jul 15;5(7):582-8. doi: 10.1021/acssynbio.6b00056. Epub 2016 May 9. PubMed |
| High Efficiency CRISPR/Cas9-mediated Gene Editing in Primary Human T-cells Using Mutant Adenoviral E4orf6/E1b55k "Helper" Proteins. Gwiazda KS, Grier AE, Sahni J, Burleigh SM, Martin U, Yang JG, Popp NA, Krutein MC, Khan IF, Jacoby K, Jensen MC, Rawlings DJ, Scharenberg AM. Mol Ther. 2016 Jun 28. doi: 10.1038/mt.2016.105. PubMed |
| CRISPR-Cas9 mediated modification of the NOD mouse genome with Ptpn22R619W mutation increases autoimmune diabetes. Lin X, Pelletier S, Gingras S, Rigaud S, Maine CJ, Marquardt K, Dai YD, Sauer K, Rodriguez AR, Martin G, Kupriyanov S, Jiang L, Yu L, Green DR, Sherman LA. Diabetes. 2016 Apr 26. pii: db160061. PubMed |
| Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series. Bowman CE, Zhao L, Hartung T, Wolfgang MJ. Mol Cell Biol. 2016 May 23. pii: MCB.00166-16. PubMed |
| Activity of Menkes Disease Protein ATP7A is Essential for Redox Balance in Mitochondria. Bhattacharjee A, Yang H, Duffy M, Robinson E, Conrad-Antoville A, Lu YW, Capps T, Braiterman LT, Wolfgang MJ, Murphy MP, Yi L, Kaler SG, Lutsenko S, Ralle M. J Biol Chem. 2016 May 16. pii: jbc.M116.727248. PubMed |
| Footprintless disruption of prosurvival genes in aneuploid cancer cells using CRISPR/Cas9 technology. Krachulec JM, Sedlmeier G, Thiele W, Sleeman JP. Biochem Cell Biol. 2016 Jun;94(3):289-96. doi: 10.1139/bcb-2015-0150. Epub 2016 Jun 2. PubMed |
Probing the impact of chromatin conformation on genome editing tools.
Chen X, Rinsma M, Janssen JM, Liu J, Maggio I, Goncalves MA.
Nucleic Acids Res. 2016 Jun 8. pii: gkw524.
PubMed
Associated Plasmids |
| Viral Determinants of miR-122-Independent Hepatitis C Virus Replication. Hopcraft SE, Azarm KD, Israelow B, Leveque N, Schwarz MC, Hsu TH, Chambers MT, Sourisseau M, Semler BL, Evans MJ. mSphere. 2015 Nov 25;1(1). pii: e00009-15. doi: 10.1128/mSphere.00009-15. eCollection 2016 Jan-Feb. PubMed |
| Engineering of Isogenic Cells Deficient for MR1 with a CRISPR/Cas9 Lentiviral System: Tools To Study Microbial Antigen Processing and Presentation to Human MR1-Restricted T Cells. Laugel B, Lloyd A, Meermeier EW, Crowther MD, Connor TR, Dolton G, Miles JJ, Burrows SR, Gold MC, Lewinsohn DM, Sewell AK. J Immunol. 2016 Jun 15. pii: 1501402. PubMed |
| Identifying and retargeting transcriptional hot spots in the human genome. Cheng JK, Lewis AM, Kim DS, Dyess T, Alper HS. Biotechnol J. 2016 Jun 17. doi: 10.1002/biot.201600015. PubMed |
| CRISPR-Cas9-mediated efficient directed mutagenesis and RAD51-dependent and RAD51-independent gene targeting in the moss Physcomitrella patens. Collonnier C, Epert A, Mara K, Maclot F, Guyon-Debast A, Charlot F, White C, Schaefer DG, Nogue F. Plant Biotechnol J. 2016 Jul 1. doi: 10.1111/pbi.12596. PubMed |
| Preparation and Analysis of Native Chromatin-Modifying Complexes. Doyon Y, Cote J. Methods Enzymol. 2016;573:303-18. doi: 10.1016/bs.mie.2016.01.017. Epub 2016 Mar 16. PubMed |
| Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens. Marceau CD, Puschnik AS, Majzoub K, Ooi YS, Brewer SM, Fuchs G, Swaminathan K, Mata MA, Elias JE, Sarnow P, Carette JE. Nature. 2016 Jul 7;535(7610):159-63. doi: 10.1038/nature18631. Epub 2016 Jun 17. PubMed |
| Loss of LSR affects epithelial barrier integrity and tumor xenograft growth of CaCo-2 cells. Czulkies BA, Mastroianni J, Lutz L, Lang S, Schwan C, Schmidt G, Lassmann S, Zeiser R, Aktories K, Papatheodorou P. Oncotarget. 2017 Jun 6;8(23):37009-37022. doi: 10.18632/oncotarget.10425. PubMed |
| Microinjection-based generation of mutant mice with a double mutation and a 0.5 Mb deletion in their genome by the CRISPR/Cas9 system. Hara S, Kato T, Goto Y, Kubota S, Tamano M, Terao M, Takada S. J Reprod Dev. 2016 Oct 18;62(5):531-536. doi: 10.1262/jrd.2016-058. Epub 2016 Jul 8. PubMed |
| ARCN1 Mutations Cause a Recognizable Craniofacial Syndrome Due to COPI-Mediated Transport Defects. Izumi K, Brett M, Nishi E, Drunat S, Tan ES, Fujiki K, Lebon S, Cham B, Masuda K, Arakawa M, Jacquinet A, Yamazumi Y, Chen ST, Verloes A, Okada Y, Katou Y, Nakamura T, Akiyama T, Gressens P, Foo R, Passemard S, Tan EC, El Ghouzzi V, Shirahige K. Am J Hum Genet. 2016 Aug 4;99(2):451-9. doi: 10.1016/j.ajhg.2016.06.011. Epub 2016 Jul 28. PubMed |
A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells.
Howden SE, McColl B, Glaser A, Vadolas J, Petrou S, Little MH, Elefanty AG, Stanley EG.
Stem Cell Reports. 2016 Sep 13;7(3):508-17. doi: 10.1016/j.stemcr.2016.07.001. Epub 2016 Aug 4.
PubMed
Associated Plasmids |
| The Dual NOD1/NOD2 Agonism of Muropeptides Containing a Meso-Diaminopimelic Acid Residue. Dagil YA, Arbatsky NP, Alkhazova BI, L'vov VL, Mazurov DV, Pashenkov MV. PLoS One. 2016 Aug 11;11(8):e0160784. doi: 10.1371/journal.pone.0160784. eCollection 2016. PubMed |
| Application of NanoLuc to monitor the intrinsic promoter activity of GRP78 using the CRISPR/Cas9 system. Oh-Hashi K, Furuta E, Norisada J, Amaya F, Hirata Y, Kiuchi K. Genes Cells. 2016 Oct;21(10):1137-1143. doi: 10.1111/gtc.12401. Epub 2016 Aug 12. PubMed |
| Human TRAV1-2-negative MR1-restricted T cells detect S. pyogenes and alternatives to MAIT riboflavin-based antigens. Meermeier EW, Laugel BF, Sewell AK, Corbett AJ, Rossjohn J, McCluskey J, Harriff MJ, Franks T, Gold MC, Lewinsohn DM. Nat Commun. 2016 Aug 16;7:12506. doi: 10.1038/ncomms12506. PubMed |
Single-cell RNA-seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm.
Chu LF, Leng N, Zhang J, Hou Z, Mamott D, Vereide DT, Choi J, Kendziorski C, Stewart R, Thomson JA.
Genome Biol. 2016 Aug 17;17(1):173. doi: 10.1186/s13059-016-1033-x.
PubMed
Associated Plasmids |
| CRISPR/Cas9 Targeted Gene Editing and Cellular Engineering in Fanconi Anemia. Osborn M, Lonetree CL, Webber BR, Patel D, Dunmire S, McElroy AN, DeFeo AP, MacMillan ML, Wagner J, Balzar BR, Tolar J. Stem Cells Dev. 2016 Aug 18. PubMed |
| Analysis of gene repair tracts from Cas9/gRNA double-stranded breaks in the human CFTR gene. Hollywood JA, Lee CM, Scallan MF, Harrison PT. Sci Rep. 2016 Aug 25;6:32230. doi: 10.1038/srep32230. PubMed |
| Comprehensive protein tyrosine phosphatase mRNA profiling identifies new regulators in the progression of glioma. Bourgonje AM, Verrijp K, Schepens JT, Navis AC, Piepers JA, Palmen CB, van den Eijnden M, Hooft van Huijsduijnen R, Wesseling P, Leenders WP, Hendriks WJ. Acta Neuropathol Commun. 2016 Sep 1;4(1):96. doi: 10.1186/s40478-016-0372-x. PubMed |
GAPTrap: A Simple Expression System for Pluripotent Stem Cells and Their Derivatives.
Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E, Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ, Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG.
Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 10.1016/j.stemcr.2016.07.015.
PubMed
Associated Plasmids |
Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs.
Port F, Bullock SL.
Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 2016 Sep 5.
PubMed
Associated Plasmids |
| nc886, a non-coding RNA and suppressor of PKR, exerts an oncogenic function in thyroid cancer. Lee EK, Hong SH, Shin S, Lee HS, Lee JS, Park EJ, Choi SS, Min JW, Park D, Hwang JA, Johnson BH, Jeon SH, Kim IH, Lee YS, Lee YS. Oncotarget. 2016 Nov 15;7(46):75000-75012. doi: 10.18632/oncotarget.11852. PubMed |
| A Library of Infectious Hepatitis C Viruses with Engineered Mutations in the E2 Gene Reveals Growth-Adaptive Mutations That Modulate Interactions with Scavenger Receptor Class B Type I. Zuiani A, Chen K, Schwarz MC, White JP, Luca VC, Fremont DH, Wang D, Evans MJ, Diamond MS. J Virol. 2016 Nov 14;90(23):10499-10512. Print 2016 Dec 1. PubMed |
| Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing. Amabile A, Migliara A, Capasso P, Biffi M, Cittaro D, Naldini L, Lombardo A. Cell. 2016 Sep 22;167(1):219-232.e14. doi: 10.1016/j.cell.2016.09.006. PubMed |
| Sphingosine 1-Phosphate Activation of EGFR As a Novel Target for Meningitic Escherichia coli Penetration of the Blood-Brain Barrier. Wang X, Maruvada R, Morris AJ, Liu JO, Wolfgang MJ, Baek DJ, Bittman R, Kim KS. PLoS Pathog. 2016 Oct 6;12(10):e1005926. doi: 10.1371/journal.ppat.1005926. eCollection 2016 Oct. PubMed |
| Genomic modelling of the ESR1 Y537S mutation for evaluating function and new therapeutic approaches for metastatic breast cancer. Harrod A, Fulton J, Nguyen VT, Periyasamy M, Ramos-Garcia L, Lai CF, Metodieva G, de Giorgio A, Williams RL, Santos DB, Gomez PJ, Lin ML, Metodiev MV, Stebbing J, Castellano L, Magnani L, Coombes RC, Buluwela L, Ali S. Oncogene. 2016 Oct 17. doi: 10.1038/onc.2016.382. PubMed |
| A Single Amino Acid Dictates Protein Kinase R Susceptibility to Unrelated Viral Antagonists. Carpentier KS, Esparo NM, Child SJ, Geballe AP. PLoS Pathog. 2016 Oct 25;12(10):e1005966. doi: 10.1371/journal.ppat.1005966. eCollection 2016 Oct. PubMed |
| The STX6-VTI1B-VAMP3 complex facilitates xenophagy by regulating the fusion between recycling endosomes and autophagosomes. Nozawa T, Minowa-Nozawa A, Aikawa C, Nakagawa I. Autophagy. 2017 Jan 2;13(1):57-69. doi: 10.1080/15548627.2016.1241924. Epub 2016 Oct 28. PubMed |
| Cas9 in Genetically Modified Food Is Unlikely to Cause Food Allergy. Nakajima O, Nishimaki-Mogami T, Kondo K. Biol Pharm Bull. 2016;39(11):1876-1880. PubMed |
| CRISPR/Cas9 editing of induced pluripotent stem cells for engineering inflammation-resistant tissues. Brunger JM, Zutshi A, Willard VP, Gersbach CA, Guilak F. Arthritis Rheumatol. 2016 Nov 3. doi: 10.1002/art.39982. PubMed |
In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration.
Suzuki K, Tsunekawa Y, Hernandez-Benitez R, Wu J, Zhu J, Kim EJ, Hatanaka F, Yamamoto M, Araoka T, Li Z, Kurita M, Hishida T, Li M, Aizawa E, Guo S, Chen S, Goebl A, Soligalla RD, Qu J, Jiang T, Fu X, Jafari M, Esteban CR, Berggren WT, Lajara J, Nunez-Delicado E, Guillen P, Campistol JM, Matsuzaki F, Liu GH, Magistretti P, Zhang K, Callaway EM, Zhang K, Belmonte JC.
Nature. 2016 Dec 1;540(7631):144-149. doi: 10.1038/nature20565. Epub 2016 Nov 16.
PubMed
Associated Plasmids |
| Glycogen Synthase Kinase 3 (GSK-3)-mediated Phosphorylation of Uracil N-Glycosylase 2 (UNG2) Facilitates the Repair of Floxuridine-induced DNA Lesions and Promotes Cell Survival. Baehr CA, Huntoon CJ, Hoang SM, Jerde CR, Karnitz LM. J Biol Chem. 2016 Dec 23;291(52):26875-26885. doi: 10.1074/jbc.M116.746081. Epub 2016 Nov 14. PubMed |
| Deletion of conserved sequences in IG-DMR at Dlk1-Gtl2 locus suggests their involvement in expression of paternally expressed genes in mice. Saito T, Hara S, Tamano M, Asahara H, Takada S. J Reprod Dev. 2016 Dec 1. PubMed |
| Two subunits of human ORC are dispensable for DNA replication and proliferation. Shibata E, Kiran M, Shibata Y, Singh S, Kiran S, Dutta A. Elife. 2016 Dec 1;5. pii: e19084. doi: 10.7554/eLife.19084. PubMed |
| Tumor Interferon Signaling Regulates a Multigenic Resistance Program to Immune Checkpoint Blockade. Benci JL, Xu B, Qiu Y, Wu TJ, Dada H, Twyman-Saint Victor C, Cucolo L, Lee DS, Pauken KE, Huang AC, Gangadhar TC, Amaravadi RK, Schuchter LM, Feldman MD, Ishwaran H, Vonderheide RH, Maity A, Wherry EJ, Minn AJ. Cell. 2016 Dec 1;167(6):1540-1554.e12. doi: 10.1016/j.cell.2016.11.022. PubMed |
| Genome-wide screen of cell-cycle regulators in normal and tumor cells identifies a differential response to nucleosome depletion. Sokolova M, Turunen M, Mortusewicz O, Kivioja T, Herr P, Vaharautio A, Bjorklund M, Taipale M, Helleday T, Taipale J. Cell Cycle. 2017 Jan 17;16(2):189-199. doi: 10.1080/15384101.2016.1261765. Epub 2016 Dec 8. PubMed |
| Knockout of a difficult-to-remove CHO host cell protein, lipoprotein lipase, for improved polysorbate stability in monoclonal antibody formulations. Chiu J, Valente KN, Levy NE, Min L, Lenhoff AM, Lee KH. Biotechnol Bioeng. 2017 May;114(5):1006-1015. doi: 10.1002/bit.26237. Epub 2016 Dec 27. PubMed |
| Overlapping roles for PARP1 and PARP2 in the recruitment of endogenous XRCC1 and PNKP into oxidized chromatin. Hanzlikova H, Gittens W, Krejcikova K, Zeng Z, Caldecott KW. Nucleic Acids Res. 2016 Dec 13. pii: gkw1246. PubMed |
Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids.
Andersson-Rolf A, Merenda A, Mustata RC, Li T, Dietmann S, Koo BK.
Dev Biol. 2016 Oct 27. pii: S0012-1606(16)30479-1. doi: 10.1016/j.ydbio.2016.10.016.
PubMed
Associated Plasmids |
| XRCC1 mutation is associated with PARP1 hyperactivation and cerebellar ataxia. Hoch NC, Hanzlikova H, Rulten SL, Tetreault M, Komulainen E, Ju L, Hornyak P, Zeng Z, Gittens W, Rey SA, Staras K, Mancini GM, McKinnon PJ, Wang ZQ, Wagner JD, Yoon G, Caldecott KW. Nature. 2017 Jan 5;541(7635):87-91. doi: 10.1038/nature20790. Epub 2016 Dec 21. PubMed |
| Generation of Hoxc13 knockout pigs recapitulates human ectodermal dysplasia-9. Han K, Liang L, Li L, Ouyang Z, Zhao B, Wang Q, Liu Z, Zhao Y, Ren X, Jiang F, Lai C, Wang K, Yan S, Huang L, Guo L, Zeng K, Lai L, Fan N. Hum Mol Genet. 2016 Dec 22. pii: ddw378. doi: 10.1093/hmg/ddw378. PubMed |
| Bcl-xL Affects Group A Streptococcus-Induced Autophagy Directly, by Inhibiting Fusion between Autophagosomes and Lysosomes, and Indirectly, by Inhibiting Bacterial Internalization via Interaction with Beclin 1-UVRAG. Nakajima S, Aikawa C, Nozawa T, Minowa-Nozawa A, Toh H, Nakagawa I. PLoS One. 2017 Jan 13;12(1):e0170138. doi: 10.1371/journal.pone.0170138. eCollection 2017. PubMed |
| RNA Helicase DDX5 Inhibits Reprogramming to Pluripotency by miRNA-Based Repression of RYBP and its PRC1-Dependent and -Independent Functions. Li H, Lai P, Jia J, Song Y, Xia Q, Huang K, He N, Ping W, Chen J, Yang Z, Li J, Yao M, Dong X, Zhao J, Hou C, Esteban MA, Gao S, Pei D, Hutchins AP, Yao H. Cell Stem Cell. 2017 Apr 6;20(4):462-477.e6. doi: 10.1016/j.stem.2016.12.002. Epub 2017 Jan 19. PubMed |
| A semisynthetic organism engineered for the stable expansion of the genetic alphabet. Zhang Y, Lamb BM, Feldman AW, Zhou AX, Lavergne T, Li L, Romesberg FE. Proc Natl Acad Sci U S A. 2017 Feb 7;114(6):1317-1322. doi: 10.1073/pnas.1616443114. Epub 2017 Jan 23. PubMed |
| CRISPR/Cas9-Induced (CTGCAG)n Repeat Instability in the Myotonic Dystrophy Type 1 Locus: Implications for Therapeutic Genome Editing. van Agtmaal EL, Andre LM, Willemse M, Cumming SA, van Kessel IDG, van den Broek WJAA, Gourdon G, Furling D, Mouly V, Monckton DG, Wansink DG, Wieringa B. Mol Ther. 2017 Jan 4;25(1):24-43. doi: 10.1016/j.ymthe.2016.10.014. Epub 2017 Jan 4. PubMed |
One-step generation of conditional and reversible gene knockouts.
Andersson-Rolf A, Mustata RC, Merenda A, Kim J, Perera S, Grego T, Andrews K, Tremble K, Silva JC, Fink J, Skarnes WC, Koo BK.
Nat Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156.
PubMed
Associated Plasmids |
Enhancement of single guide RNA transcription for efficient CRISPR/Cas-based genomic engineering.
Ui-Tei K, Maruyama S, Nakano Y.
Genome. 2017 Jun;60(6):537-545. doi: 10.1139/gen-2016-0127. Epub 2017 Jan 26.
PubMed
Associated Plasmids |
| Stage-Specific Human Induced Pluripotent Stem Cells Map the Progression of Myeloid Transformation to Transplantable Leukemia. Kotini AG, Chang CJ, Chow A, Yuan H, Ho TC, Wang T, Vora S, Solovyov A, Husser C, Olszewska M, Teruya-Feldstein J, Perumal D, Klimek VM, Spyridonidis A, Rampal RK, Silverman L, Reddy EP, Papaemmanuil E, Parekh S, Greenbaum BD, Leslie CS, Kharas MG, Papapetrou EP. Cell Stem Cell. 2017 Mar 2;20(3):315-328.e7. doi: 10.1016/j.stem.2017.01.009. Epub 2017 Feb 16. PubMed |
A stable but reversible integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair.
Wen Y, Liao G, Pritchard T, Zhao TT, Connelly JP, Pruett-Miller SM, Blanc V, Davidson NO, Madison BB.
J Biol Chem. 2017 Apr 14;292(15):6148-6162. doi: 10.1074/jbc.M117.777722. Epub 2017 Feb 22.
PubMed
Associated Plasmids |
| ARID2 modulates DNA damage response in human hepatocellular carcinoma cells. Oba A, Shimada S, Akiyama Y, Nishikawaji T, Mogushi K, Ito H, Matsumura S, Aihara A, Mitsunori Y, Ban D, Ochiai T, Kudo A, Asahara H, Kaida A, Miura M, Tanabe M, Tanaka S. J Hepatol. 2017 May;66(5):942-951. doi: 10.1016/j.jhep.2016.12.026. Epub 2017 Feb 24. PubMed |
| CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa. Webber BR, Osborn MJ, McElroy AN, Twaroski K, Lonetree CL, DeFeo AP, Xia L, Eide C, Lees CJ, McElmurry RT, Riddle MJ, Kim CJ, Patel DD, Blazar BR, Tolar J. NPJ Regen Med. 2016;1. doi: 10.1038/npjregenmed.2016.14. Epub 2016 Dec 8. PubMed |
| Genetic dissection of colorectal cancer progression by orthotopic transplantation of engineered cancer organoids. Fumagalli A, Drost J, Suijkerbuijk SJ, van Boxtel R, de Ligt J, Offerhaus GJ, Begthel H, Beerling E, Tan EH, Sansom OJ, Cuppen E, Clevers H, van Rheenen J. Proc Natl Acad Sci U S A. 2017 Mar 21;114(12):E2357-E2364. doi: 10.1073/pnas.1701219114. Epub 2017 Mar 7. PubMed |
Dual-utility NLS drives RNF169-dependent DNA damage responses.
An L, Jiang Y, Ng HH, Man EP, Chen J, Khoo US, Gong Q, Huen MS.
Proc Natl Acad Sci U S A. 2017 Apr 4;114(14):E2872-E2881. doi: 10.1073/pnas.1616602114. Epub 2017 Mar 21.
PubMed
Associated Plasmids |
| cis-Regulatory Circuits Regulating NEK6 Kinase Overexpression in Transformed B Cells Are Super-Enhancer Independent. Huang Y, Koues OI, Zhao JY, Liu R, Pyfrom SC, Payton JE, Oltz EM. Cell Rep. 2017 Mar 21;18(12):2918-2931. doi: 10.1016/j.celrep.2017.02.067. PubMed |
| Mechanisms of precise genome editing using oligonucleotide donors. Kan Y, Ruis B, Takasugi T, Hendrickson EA. Genome Res. 2017 Jul;27(7):1099-1111. doi: 10.1101/gr.214775.116. Epub 2017 Mar 29. PubMed |
| Exploiting induced pluripotent stem cell-derived macrophages to unravel host factors influencing Chlamydia trachomatis pathogenesis. Yeung ATY, Hale C, Lee AH, Gill EE, Bushell W, Parry-Smith D, Goulding D, Pickard D, Roumeliotis T, Choudhary J, Thomson N, Skarnes WC, Dougan G, Hancock REW. Nat Commun. 2017 Apr 25;8:15013. doi: 10.1038/ncomms15013. PubMed |
| Genome Engineering of Stem Cells for Autonomously Regulated, Closed-Loop Delivery of Biologic Drugs. Brunger JM, Zutshi A, Willard VP, Gersbach CA, Guilak F. Stem Cell Reports. 2017 May 9;8(5):1202-1213. doi: 10.1016/j.stemcr.2017.03.022. Epub 2017 Apr 27. PubMed |
| Metformin inhibits SUV39H1-mediated migration of prostate cancer cells. Yu T, Wang C, Yang J, Guo Y, Wu Y, Li X. Oncogenesis. 2017 May 1;6(5):e324. doi: 10.1038/oncsis.2017.28. PubMed |
| SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function. Coleman KE, Bekes M, Chapman JR, Crist SB, Jones MJ, Ueberheide BM, Huang TT. Elife. 2017 May 5;6. doi: 10.7554/eLife.24325. PubMed |
| The Mammalian Malonyl-CoA Synthetase ACSF3 Is Required for Mitochondrial Protein Malonylation and Metabolic Efficiency. Bowman CE, Rodriguez S, Selen Alpergin ES, Acoba MG, Zhao L, Hartung T, Claypool SM, Watkins PA, Wolfgang MJ. Cell Chem Biol. 2017 Jun 22;24(6):673-684.e4. doi: 10.1016/j.chembiol.2017.04.009. Epub 2017 May 4. PubMed |
| MK2 Phosphorylates RIPK1 to Prevent TNF-Induced Cell Death. Jaco I, Annibaldi A, Lalaoui N, Wilson R, Tenev T, Laurien L, Kim C, Jamal K, Wicky John S, Liccardi G, Chau D, Murphy JM, Brumatti G, Feltham R, Pasparakis M, Silke J, Meier P. Mol Cell. 2017 Jun 1;66(5):698-710.e5. doi: 10.1016/j.molcel.2017.05.003. Epub 2017 May 11. PubMed |
| PD-L1 expression on malignant cells is no prerequisite for checkpoint therapy. Kleinovink JW, Marijt KA, Schoonderwoerd MJA, van Hall T, Ossendorp F, Fransen MF. Oncoimmunology. 2017 Feb 21;6(4):e1294299. doi: 10.1080/2162402X.2017.1294299. eCollection 2017. PubMed |
| Prosurvival long noncoding RNA PINCR regulates a subset of p53 targets in human colorectal cancer cells by binding to Matrin 3. Chaudhary R, Gryder B, Woods WS, Subramanian M, Jones MF, Li XL, Jenkins LM, Shabalina SA, Mo M, Dasso M, Yang Y, Wakefield LM, Zhu Y, Frier SM, Moriarity BS, Prasanth KV, Perez-Pinera P, Lal A. Elife. 2017 Jun 5;6. doi: 10.7554/eLife.23244. PubMed |
| CRISPR-STOP: gene silencing through base-editing-induced nonsense mutations. Kuscu C, Parlak M, Tufan T, Yang J, Szlachta K, Wei X, Mammadov R, Adli M. Nat Methods. 2017 Jul;14(7):710-712. doi: 10.1038/nmeth.4327. Epub 2017 Jun 5. PubMed |
| Locus-specific ChIP combined with NGS analysis reveals genomic regulatory regions that physically interact with the Pax5 promoter in a chicken B cell line. Fujita T, Kitaura F, Yuno M, Suzuki Y, Sugano S, Fujii H. DNA Res. 2017 Jun 6. doi: 10.1093/dnares/dsx023. PubMed |
| Identification of the elementary structural units of the DNA damage response. Natale F, Rapp A, Yu W, Maiser A, Harz H, Scholl A, Grulich S, Anton T, Horl D, Chen W, Durante M, Taucher-Scholz G, Leonhardt H, Cardoso MC. Nat Commun. 2017 Jun 12;8:15760. doi: 10.1038/ncomms15760. PubMed |
| Genome Editing Mediated by Primordial Germ Cell in Chicken. Han JY, Lee HJ. Methods Mol Biol. 2017;1630:153-163. doi: 10.1007/978-1-4939-7128-2_13. PubMed |
| Synthetic peptide TEKKRRETVEREKE derived from ezrin induces differentiation of NIH/3T3 fibroblasts. Chulkina M, Negmadjanov U, Lebedeva E, Pichugin A, Mazurov D, Ataullakhanov R, Holmuhamedov E. Eur J Pharmacol. 2017 Jun 28. pii: S0014-2999(17)30441-7. doi: 10.1016/j.ejphar.2017.06.033. PubMed |
| N-terminal part of Dishevelled DEP domain is required for Wnt/beta-catenin signaling in mammalian cells. Paclikova P, Bernatik O, Radaszkiewicz TW, Bryja V. Mol Cell Biol. 2017 Jul 3. pii: MCB.00145-17. doi: 10.1128/MCB.00145-17. PubMed |
| Entosis Is Induced by Glucose Starvation. Hamann JC, Surcel A, Chen R, Teragawa C, Albeck JG, Robinson DN, Overholtzer M. Cell Rep. 2017 Jul 5;20(1):201-210. doi: 10.1016/j.celrep.2017.06.037. PubMed |
| A Role for Mitochondrial Translation in Promotion of Viability in K-Ras Mutant Cells. Martin TD, Cook DR, Choi MY, Li MZ, Haigis KM, Elledge SJ. Cell Rep. 2017 Jul 11;20(2):427-438. doi: 10.1016/j.celrep.2017.06.061. PubMed |
Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics.
Rose JC, Stephany JJ, Valente WJ, Trevillian BM, Dang HV, Bielas JH, Maly DJ, Fowler DM.
Nat Methods. 2017 Jul 24. doi: 10.1038/nmeth.4368.
PubMed
Associated Plasmids |
| Ubiquitination of exposed glycoproteins by SCF(FBXO27) directs damaged lysosomes for autophagy. Yoshida Y, Yasuda S, Fujita T, Hamasaki M, Murakami A, Kawawaki J, Iwai K, Saeki Y, Yoshimori T, Matsuda N, Tanaka K. Proc Natl Acad Sci U S A. 2017 Aug 8;114(32):8574-8579. doi: 10.1073/pnas.1702615114. Epub 2017 Jul 25. PubMed |
| A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer. Merenda A, Andersson-Rolf A, Mustata RC, Li T, Kim H, Koo BK. J Vis Exp. 2017 Jul 12;(125). doi: 10.3791/55916. PubMed |
| Agonist-induced dimer dissociation as a macromolecular step in G protein-coupled receptor signaling. Petersen J, Wright SC, Rodriguez D, Matricon P, Lahav N, Vromen A, Friedler A, Stromqvist J, Wennmalm S, Carlsson J, Schulte G. Nat Commun. 2017 Aug 9;8(1):226. doi: 10.1038/s41467-017-00253-9. PubMed |
| CRISPR/Cas9-Mediated Deletion of Foxn1 in NOD/SCID/IL2rg(-/-) Mice Results in Severe Immunodeficiency. Wei X, Lai Y, Li B, Qin L, Xu Y, Lin S, Wang S, Wu Q, Liang Q, Huang G, Deng Q, Liu P, Wu D, Lai L, Yao Y, Li P. Sci Rep. 2017 Aug 10;7(1):7720. doi: 10.1038/s41598-017-08337-8. PubMed |
| TH2A is phosphorylated at meiotic centromere by Haspin. Hada M, Kim J, Inoue E, Fukuda Y, Tanaka H, Watanabe Y, Okada Y. Chromosoma. 2017 Dec;126(6):769-780. doi: 10.1007/s00412-017-0638-5. Epub 2017 Aug 12. PubMed |
| Human microcephaly protein RTTN interacts with STIL and is required to build full-length centrioles. Chen HY, Wu CT, Tang CC, Lin YN, Wang WJ, Tang TK. Nat Commun. 2017 Aug 15;8(1):247. doi: 10.1038/s41467-017-00305-0. PubMed |
| Cell Cycle-Related Kinase (CCRK) regulates ciliogenesis and Hedgehog signaling in mice. Snouffer A, Brown D, Lee H, Walsh J, Lupu F, Norman R, Lechtreck K, Ko HW, Eggenschwiler J. PLoS Genet. 2017 Aug 17;13(8):e1006912. doi: 10.1371/journal.pgen.1006912. eCollection 2017 Aug. PubMed |
| Functional validation of ATF4 and GADD34 in Neuro2a cells by CRISPR/Cas9-mediated genome editing. Oh-Hashi K, Sugiura N, Amaya F, Isobe KI, Hirata Y. Mol Cell Biochem. 2018 Mar;440(1-2):65-75. doi: 10.1007/s11010-017-3156-0. Epub 2017 Aug 19. PubMed |
| Rab35 GTPase recruits NDP52 to autophagy targets. Minowa-Nozawa A, Nozawa T, Okamoto-Furuta K, Kohda H, Nakagawa I. EMBO J. 2017 Sep 15;36(18):2790-2807. doi: 10.15252/embj.201796463. Epub 2017 Aug 28. PubMed |
| The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes. Castonguay J, Orth JHC, Muller T, Sleman F, Grimm C, Wahl-Schott C, Biel M, Mallmann RT, Bildl W, Schulte U, Klugbauer N. Sci Rep. 2017 Aug 30;7(1):10038. doi: 10.1038/s41598-017-10607-4. PubMed |
| Reconstruction of enhancer-target networks in 935 samples of human primary cells, tissues and cell lines. Cao Q, Anyansi C, Hu X, Xu L, Xiong L, Tang W, Mok MTS, Cheng C, Fan X, Gerstein M, Cheng ASL, Yip KY. Nat Genet. 2017 Oct;49(10):1428-1436. doi: 10.1038/ng.3950. Epub 2017 Sep 4. PubMed |
| Mitochondrial fission facilitates the selective mitophagy of protein aggregates. Burman JL, Pickles S, Wang C, Sekine S, Vargas JNS, Zhang Z, Youle AM, Nezich CL, Wu X, Hammer JA, Youle RJ. J Cell Biol. 2017 Oct 2;216(10):3231-3247. doi: 10.1083/jcb.201612106. Epub 2017 Sep 11. PubMed |
| BRCA2 suppresses replication stress-induced mitotic and G1 abnormalities through homologous recombination. Feng W, Jasin M. Nat Commun. 2017 Sep 13;8(1):525. doi: 10.1038/s41467-017-00634-0. PubMed |
| mTOR Controls Mitochondrial Dynamics and Cell Survival via MTFP1. Morita M, Prudent J, Basu K, Goyon V, Katsumura S, Hulea L, Pearl D, Siddiqui N, Strack S, McGuirk S, St-Pierre J, Larsson O, Topisirovic I, Vali H, McBride HM, Bergeron JJ, Sonenberg N. Mol Cell. 2017 Sep 21;67(6):922-935.e5. doi: 10.1016/j.molcel.2017.08.013. Epub 2017 Sep 14. PubMed |
Generation of an OCT4 reporter human induced pluripotent stem cell line using CRISPR/SpCas9.
Balboa D, Weltner J, Novik Y, Eurola S, Wartiovaara K, Otonkoski T.
Stem Cell Res. 2017 Aug;23:105-108. doi: 10.1016/j.scr.2017.07.006. Epub 2017 Jul 11.
PubMed
Associated Plasmids |
| A novel requirement for DROSHA in maintenance of mammalian CG methylation. Stathopoulou A, Chhetri JB, Ambrose JC, Esteve PO, Ji L, Erdjument-Bromage H, Zhang G, Neubert TA, Pradhan S, Herrero J, Schmitz RJ, Ooi SKT. Nucleic Acids Res. 2017 Sep 19;45(16):9398-9412. doi: 10.1093/nar/gkx695. PubMed |
| Application of a novel HiBiT peptide tag for monitoring ATF4 protein expression in Neuro2a cells. Oh-Hashi K, Furuta E, Fujimura K, Hirata Y. Biochem Biophys Rep. 2017 Aug 15;12:40-45. doi: 10.1016/j.bbrep.2017.08.002. eCollection 2017 Dec. PubMed |
| CRISPR/Cas9-mediated simultaneous knockout of Dmrt1 and Dmrt3 does not recapitulate the 46,XY gonadal dysgenesis observed in 9p24.3 deletion patients. Inui M, Tamano M, Kato T, Takada S. Biochem Biophys Rep. 2017 Jan 9;9:238-244. doi: 10.1016/j.bbrep.2017.01.001. eCollection 2017 Mar. PubMed |
| p53 controls expression of the DNA deaminase APOBEC3B to limit its potential mutagenic activity in cancer cells. Periyasamy M, Singh AK, Gemma C, Kranjec C, Farzan R, Leach DA, Navaratnam N, Palinkas HL, Vertessy BG, Fenton TR, Doorbar J, Fuller-Pace F, Meek DW, Coombes RC, Buluwela L, Ali S. Nucleic Acids Res. 2017 Nov 2;45(19):11056-11069. doi: 10.1093/nar/gkx721. PubMed |
| Generation of three miR-122 knockout lines from a human embryonic stem cell line. Liu Y, Wu F, Zhuang Y, Guo D, Abbas N, Wei R, Wang K, Chen Y, Tao J, Wu Y, Yuan F, Pan T, Yang F, Lai K, Lai L, Li YX. Stem Cell Res. 2017 Oct;24:164-168. doi: 10.1016/j.scr.2017.06.004. Epub 2017 Jun 9. PubMed |
| Generation of two MEN1 knockout lines from a human embryonic stem cell line. Liu Y, Ruzi A, Guo D, Wu F, Liu H, Wu H, Wu Y, Yuan F, Zhuang Y, Yang F, Lai K, Lai L, Li YX. Stem Cell Res. 2017 Oct;24:169-173. doi: 10.1016/j.scr.2017.06.005. Epub 2017 Jun 10. PubMed |
| Generation of a KCNJ11 homozygous knockout human embryonic stem cell line WAe001-A-12 using CRISPR/Cas9. Yuan F, Guo D, Gao G, Liu Y, Xu Y, Wu Y, Yang F, Ke X, Lai K, Hong L, Li YX. Stem Cell Res. 2017 Oct;24:89-93. doi: 10.1016/j.scr.2017.08.016. Epub 2017 Aug 30. PubMed |
| Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca(2+)-ATPases and Key Regulators of Ca(2+) Clearance. Schmidt N, Kollewe A, Constantin CE, Henrich S, Ritzau-Jost A, Bildl W, Saalbach A, Hallermann S, Kulik A, Fakler B, Schulte U. Neuron. 2017 Nov 15;96(4):827-838.e9. doi: 10.1016/j.neuron.2017.09.038. Epub 2017 Oct 19. PubMed |
| Methylation of transcription factor YY2 regulates its transcriptional activity and cell proliferation. Wu XN, Shi TT, He YH, Wang FF, Sang R, Ding JC, Zhang WJ, Shu XY, Shen HF, Yi J, Gao X, Liu W. Cell Discov. 2017 Oct 3;3:17035. doi: 10.1038/celldisc.2017.35. eCollection 2017. PubMed |
| Methods for Scarless, Selection-Free Generation of Human Cells and Allele-Specific Functional Analysis of Disease-Associated SNPs and Variants of Uncertain Significance. Coggins NB, Stultz J, O'Geen H, Carvajal-Carmona LG, Segal DJ. Sci Rep. 2017 Nov 8;7(1):15044. doi: 10.1038/s41598-017-15407-4. PubMed |
| DNA methylation defines regional identity of human intestinal epithelial organoids and undergoes dynamic changes during development. Kraiczy J, Nayak KM, Howell KJ, Ross A, Forbester J, Salvestrini C, Mustata R, Perkins S, Andersson-Rolf A, Leenen E, Liebert A, Vallier L, Rosenstiel PC, Stegle O, Dougan G, Heuschkel R, Koo BK, Zilbauer M. Gut. 2019 Jan;68(1):49-61. doi: 10.1136/gutjnl-2017-314817. Epub 2017 Nov 15. PubMed |
| A Universal Approach to Correct Various HBB Gene Mutations in Human Stem Cells for Gene Therapy of Beta-Thalassemia and Sickle Cell Disease. Cai L, Bai H, Mahairaki V, Gao Y, He C, Wen Y, Jin YC, Wang Y, Pan RL, Qasba A, Ye Z, Cheng L. Stem Cells Transl Med. 2018 Jan;7(1):87-97. doi: 10.1002/sctm.17-0066. Epub 2017 Nov 21. PubMed |
| Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes. Sato M, Kosuke M, Koriyama M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Miyoshi K. Theriogenology. 2018 Mar 1;108:29-38. doi: 10.1016/j.theriogenology.2017.11.030. Epub 2017 Nov 26. PubMed |
| Optimized guide RNA structure for genome editing via Cas9. Xu J, Lian W, Jia Y, Li L, Huang Z. Oncotarget. 2017 Oct 7;8(55):94166-94171. doi: 10.18632/oncotarget.21607. eCollection 2017 Nov 7. PubMed |
| Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9. Yuan F, Guo D, Liu Y, Xu Y, Gao G, Wu Y, Yang F, Ke X, Lai K, Wei H, Li YX. Stem Cell Res. 2018 Jan;26:67-71. doi: 10.1016/j.scr.2017.11.007. Epub 2017 Nov 10. PubMed |
| YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration. Yui S, Azzolin L, Maimets M, Pedersen MT, Fordham RP, Hansen SL, Larsen HL, Guiu J, Alves MRP, Rundsten CF, Johansen JV, Li Y, Madsen CD, Nakamura T, Watanabe M, Nielsen OH, Schweiger PJ, Piccolo S, Jensen KB. Cell Stem Cell. 2018 Jan 4;22(1):35-49.e7. doi: 10.1016/j.stem.2017.11.001. Epub 2017 Dec 14. PubMed |
| Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing. Wu F, Gao G, Pan T, Yang Z, Xu G, Abbas N, Liu Y, Chen Y, Tan S, You K, Ke X, Zhuang Y, Lin X, Yang F, Li YX. Stem Cell Res. 2018 Mar;27:5-9. doi: 10.1016/j.scr.2017.12.002. Epub 2017 Dec 5. PubMed |
| Human-Specific Adaptations in Vpu Conferring Anti-tetherin Activity Are Critical for Efficient Early HIV-1 Replication In Vivo. Yamada E, Nakaoka S, Klein L, Reith E, Langer S, Hopfensperger K, Iwami S, Schreiber G, Kirchhoff F, Koyanagi Y, Sauter D, Sato K. Cell Host Microbe. 2018 Jan 10;23(1):110-120.e7. doi: 10.1016/j.chom.2017.12.009. PubMed |
| Functional analysis after rapid degradation of condensins and 3D-EM reveals chromatin volume is uncoupled from chromosome architecture in mitosis. Samejima K, Booth DG, Ogawa H, Paulson JR, Xie L, Watson CA, Platani M, Kanemaki MT, Earnshaw WC. J Cell Sci. 2018 Feb 22;131(4). pii: jcs.210187. doi: 10.1242/jcs.210187. PubMed |
| T cells specific for a TAP-independent self-peptide remain naive in tumor-bearing mice and are fully exploitable for therapy. Doorduijn EM, Sluijter M, Marijt KA, Querido BJ, van der Burg SH, van Hall T. Oncoimmunology. 2017 Nov 20;7(3):e1382793. doi: 10.1080/2162402X.2017.1382793. eCollection 2018. PubMed |
| High Efficiency Gene Correction in Hematopoietic Cells by Donor-Template-Free CRISPR/Cas9 Genome Editing. Surun D, Schwable J, Tomasovic A, Ehling R, Stein S, Kurrle N, von Melchner H, Schnutgen F. Mol Ther Nucleic Acids. 2018 Mar 2;10:1-8. doi: 10.1016/j.omtn.2017.11.001. Epub 2017 Nov 10. PubMed |
CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair.
Charpentier M, Khedher AHY, Menoret S, Brion A, Lamribet K, Dardillac E, Boix C, Perrouault L, Tesson L, Geny S, De Cian A, Itier JM, Anegon I, Lopez B, Giovannangeli C, Concordet JP.
Nat Commun. 2018 Mar 19;9(1):1133. doi: 10.1038/s41467-018-03475-7.
PubMed
Associated Plasmids |
Unexpected binding behaviors of bacterial Argonautes in human cells cast doubts on their use as targetable gene regulators.
O'Geen H, Ren C, Coggins NB, Bates SL, Segal DJ.
PLoS One. 2018 Mar 27;13(3):e0193818. doi: 10.1371/journal.pone.0193818. eCollection 2018.
PubMed
Associated Plasmids |
| Efficient In Vivo Liver-Directed Gene Editing Using CRISPR/Cas9. Singh K, Evens H, Nair N, Rincon MY, Sarcar S, Samara-Kuko E, Chuah MK, VandenDriessche T. Mol Ther. 2018 May 2;26(5):1241-1254. doi: 10.1016/j.ymthe.2018.02.023. Epub 2018 Mar 6. PubMed |
| Functional Insulator Scanning of CpG Islands to Identify Regulatory Regions of Promoters Using CRISPR. Grob A, Marbiah M, Isalan M. Methods Mol Biol. 2018;1766:285-301. doi: 10.1007/978-1-4939-7768-0_16. PubMed |
| A Huntingtin Knockin Pig Model Recapitulates Features of Selective Neurodegeneration in Huntington's Disease. Yan S, Tu Z, Liu Z, Fan N, Yang H, Yang S, Yang W, Zhao Y, Ouyang Z, Lai C, Yang H, Li L, Liu Q, Shi H, Xu G, Zhao H, Wei H, Pei Z, Li S, Lai L, Li XJ. Cell. 2018 May 3;173(4):989-1002.e13. doi: 10.1016/j.cell.2018.03.005. Epub 2018 Mar 29. PubMed |
| JMJD6 Licenses ERalpha-Dependent Enhancer and Coding Gene Activation by Modulating the Recruitment of the CARM1/MED12 Co-activator Complex. Gao WW, Xiao RQ, Zhang WJ, Hu YR, Peng BL, Li WJ, He YH, Shen HF, Ding JC, Huang QX, Ye TY, Li Y, Liu ZY, Ding R, Rosenfeld MG, Liu W. Mol Cell. 2018 Apr 19;70(2):340-357.e8. doi: 10.1016/j.molcel.2018.03.006. Epub 2018 Apr 5. PubMed |
| Reproductive technologies for the generation and maintenance of valuable animal strains. Kaneko T. J Reprod Dev. 2018 Jun 22;64(3):209-215. doi: 10.1262/jrd.2018-035. Epub 2018 Apr 14. PubMed |
| Tumor-secreted Pros1 inhibits macrophage M1 polarization to reduce antitumor immune response. Ubil E, Caskey L, Holtzhausen A, Hunter D, Story C, Earp HS. J Clin Invest. 2018 Jun 1;128(6):2356-2369. doi: 10.1172/JCI97354. Epub 2018 Apr 30. PubMed |
| A genome-wide assessment of conserved SNP alleles reveals a panel of regulatory SNPs relevant to the peripheral nerve. Law WD, Fogarty EA, Vester A, Antonellis A. BMC Genomics. 2018 May 2;19(1):311. doi: 10.1186/s12864-018-4692-z. PubMed |
| Detection of genome-edited cells by oligoribonucleotide interference-PCR. Fujita T, Yuno M, Kitaura F, Fujii H. DNA Res. 2018 Aug 1;25(4):395-407. doi: 10.1093/dnares/dsy012. PubMed |
| Targeted Gene Knock Out Using Nuclease-Assisted Vector Integration: Hemi- and Homozygous Deletion of JAG1. Gapinske M, Tague N, Winter J, Underhill GH, Perez-Pinera P. Methods Mol Biol. 2018;1772:233-248. doi: 10.1007/978-1-4939-7795-6_13. PubMed |
| In Situ Gene Therapy via AAV-CRISPR-Cas9-Mediated Targeted Gene Regulation. Moreno AM, Fu X, Zhu J, Katrekar D, Shih YV, Marlett J, Cabotaje J, Tat J, Naughton J, Lisowski L, Varghese S, Zhang K, Mali P. Mol Ther. 2018 Jul 5;26(7):1818-1827. doi: 10.1016/j.ymthe.2018.04.017. Epub 2018 Apr 25. PubMed |
| CRISPR/Cas9-mediated specific integration of fat-1 at the goat MSTN locus. Zhang J, Cui ML, Nie YW, Dai B, Li FR, Liu DJ, Liang H, Cang M. FEBS J. 2018 Aug;285(15):2828-2839. doi: 10.1111/febs.14520. Epub 2018 Jun 15. PubMed |
Efficiency and Specificity of Targeted Integration Mediated by the Adeno-Associated Virus Serotype 2 Rep 78 Protein.
Li P, Marino MP, Zou J, Argaw T, Morreale MT, Iaffaldano BJ, Reiser J.
Hum Gene Ther Methods. 2018 Jun;29(3):135-145. doi: 10.1089/hgtb.2018.052.
PubMed
Associated Plasmids |
| Super-Resolution Imaging Reveals TCTN2 Depletion-Induced IFT88 Lumen Leakage and Ciliary Weakening. Weng RR, Yang TT, Huang CE, Chang CW, Wang WJ, Liao JC. Biophys J. 2018 Jul 17;115(2):263-275. doi: 10.1016/j.bpj.2018.04.051. Epub 2018 Jun 1. PubMed |
| Application of NanoBiT for Monitoring Dimerization of the Null Hong Kong Variant of alpha-1-Antitrypsin, NHK, in Living Cells. Norisada J, Fujimura K, Amaya F, Kohno H, Hirata Y, Oh-Hashi K. Mol Biotechnol. 2018 Aug;60(8):539-549. doi: 10.1007/s12033-018-0092-5. PubMed |
| A tandem repeat array in IG-DMR is essential for imprinting of paternal allele at the Dlk1-Dio3 domain during embryonic development. Saito T, Hara S, Kato T, Tamano M, Muramatsu A, Asahara H, Takada S. Hum Mol Genet. 2018 Sep 15;27(18):3283-3292. doi: 10.1093/hmg/ddy235. PubMed |
LGP2 virus sensor regulates gene expression network mediated by TRBP-bound microRNAs.
Takahashi T, Nakano Y, Onomoto K, Murakami F, Komori C, Suzuki Y, Yoneyama M, Ui-Tei K.
Nucleic Acids Res. 2018 Sep 28;46(17):9134-9147. doi: 10.1093/nar/gky575.
PubMed
Associated Plasmids |
| Identification of an enhancer region within the TP63/LEPREL1 locus containing genetic variants associated with bladder cancer risk. Dudek AM, Vermeulen SH, Kolev D, Grotenhuis AJ, Kiemeney LALM, Verhaegh GW. Cell Oncol (Dordr). 2018 Oct;41(5):555-568. doi: 10.1007/s13402-018-0393-5. Epub 2018 Jun 28. PubMed |
| Optimized Plasmid Construction Strategy for Cas9. Xu J, Li W, Hossen MM, Jia Y, Li L, Huang Z. Cell Physiol Biochem. 2018;48(1):131-137. doi: 10.1159/000491669. Epub 2018 Jul 12. PubMed |
| MUNC, an Enhancer RNA Upstream from the MYOD Gene, Induces a Subgroup of Myogenic Transcripts in trans Independently of MyoD. Cichewicz MA, Kiran M, Przanowska RK, Sobierajska E, Shibata Y, Dutta A. Mol Cell Biol. 2018 Sep 28;38(20). pii: MCB.00655-17. doi: 10.1128/MCB.00655-17. Print 2018 Oct 15. PubMed |
| Species-independent contribution of ZBP1/DAI/DLM-1-triggered necroptosis in host defense against HSV1. Guo H, Gilley RP, Fisher A, Lane R, Landsteiner VJ, Ragan KB, Dovey CM, Carette JE, Upton JW, Mocarski ES, Kaiser WJ. Cell Death Dis. 2018 Jul 26;9(8):816. doi: 10.1038/s41419-018-0868-3. PubMed |
| Confirming TDP2 mutation in spinocerebellar ataxia autosomal recessive 23 (SCAR23). Zagnoli-Vieira G, Bruni F, Thompson K, He L, Walker S, de Brouwer APM, Taylor R, Niyazov D, Caldecott KW. Neurol Genet. 2018 Aug 1;4(4):e262. doi: 10.1212/NXG.0000000000000262. eCollection 2018 Aug. PubMed |
| Expression analysis and functional characterization of the mouse cysteine-rich with EGF-like domains 2. Oh-Hashi K, Fujimura K, Norisada J, Hirata Y. Sci Rep. 2018 Aug 15;8(1):12236. doi: 10.1038/s41598-018-30362-4. PubMed |
| Advancing the predictivity of skin sensitization by applying a novel HMOX1 reporter system. Zhong G, Li H, Bai J, Pang S, He C, Du X, Wang H, Zhang Q, Xie S, Du H, Dai R, Huang L. Arch Toxicol. 2018 Oct;92(10):3103-3115. doi: 10.1007/s00204-018-2287-8. Epub 2018 Aug 21. PubMed |
| Modeling Wnt signaling by CRISPR-Cas9 genome editing recapitulates neoplasia in human Barrett epithelial organoids. Liu X, Cheng Y, Abraham JM, Wang Z, Wang Z, Ke X, Yan R, Shin EJ, Ngamruengphong S, Khashab MA, Zhang G, McNamara G, Ewald AJ, Lin D, Liu Z, Meltzer SJ. Cancer Lett. 2018 Nov 1;436:109-118. doi: 10.1016/j.canlet.2018.08.017. Epub 2018 Aug 23. PubMed |
| Disruption of the beta1L Isoform of GABP Reverses Glioblastoma Replicative Immortality in a TERT Promoter Mutation-Dependent Manner. Mancini A, Xavier-Magalhaes A, Woods WS, Nguyen KT, Amen AM, Hayes JL, Fellmann C, Gapinske M, McKinney AM, Hong C, Jones LE, Walsh KM, Bell RJA, Doudna JA, Costa BM, Song JS, Perez-Pinera P, Costello JF. Cancer Cell. 2018 Sep 10;34(3):513-528.e8. doi: 10.1016/j.ccell.2018.08.003. PubMed |
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| A bispecific immunotweezer prevents soluble PrP oligomers and abolishes prion toxicity. Bardelli M, Frontzek K, Simonelli L, Hornemann S, Pedotti M, Mazzola F, Carta M, Eckhardt V, D'Antuono R, Virgilio T, Gonzalez SF, Aguzzi A, Varani L. PLoS Pathog. 2018 Oct 1;14(10):e1007335. doi: 10.1371/journal.ppat.1007335. eCollection 2018 Oct. PubMed |
| Construction of a GLI3 compound heterozygous knockout human embryonic stem cell line WAe001-A-20 by CRISPR/Cas9 editing. Wei R, Yuan F, Wu Y, Liu Y, You K, Yang Z, Chen Y, Getachew A, Wang N, Xu Y, Zhuang Y, Yang F, Li YX. Stem Cell Res. 2018 Oct;32:139-144. doi: 10.1016/j.scr.2018.09.010. Epub 2018 Sep 17. PubMed |
| Generation of a cancer testis antigen mCherry reporter HCT116 colorectal carcinoma cell line. Chhetri JB, Drousioti E, Guerra-Assuncao JA, Herrero J, Ooi SKT. Heliyon. 2018 Oct 13;4(10):e00858. doi: 10.1016/j.heliyon.2018.e00858. eCollection 2018 Oct. PubMed |
| Aptazyme-mediated direct modulation of post-transcriptional sgRNA level for conditional genome editing and gene expression. Chen H, Li Y, Du C, Li Y, Zhao J, Zheng X, Mao Q, Xia H. J Biotechnol. 2018 Dec 20;288:23-29. doi: 10.1016/j.jbiotec.2018.10.011. Epub 2018 Nov 1. PubMed |
| Dom34 mediates targeting of exogenous RNA in the antiviral OAS/RNase L pathway. Nogimori T, Nishiura K, Kawashima S, Nagai T, Oishi Y, Hosoda N, Imataka H, Kitamura Y, Kitade Y, Hoshino SI. Nucleic Acids Res. 2019 Jan 10;47(1):432-449. doi: 10.1093/nar/gky1087. PubMed |
| The neurodegenerative diseases ALS and SMA are linked at the molecular level via the ASC-1 complex. Chi B, O'Connell JD, Iocolano AD, Coady JA, Yu Y, Gangopadhyay J, Gygi SP, Reed R. Nucleic Acids Res. 2018 Dec 14;46(22):11939-11951. doi: 10.1093/nar/gky1093. PubMed |
| LAMTOR2/LAMTOR1 complex is required for TAX1BP1-mediated xenophagy. Lin CY, Nozawa T, Minowa-Nozawa A, Toh H, Aikawa C, Nakagawa I. Cell Microbiol. 2019 Apr;21(4):e12981. doi: 10.1111/cmi.12981. Epub 2019 Jan 21. PubMed |
| Protocadherin-1 is essential for cell entry by New World hantaviruses. Jangra RK, Herbert AS, Li R, Jae LT, Kleinfelter LM, Slough MM, Barker SL, Guardado-Calvo P, Roman-Sosa G, Dieterle ME, Kuehne AI, Muena NA, Wirchnianski AS, Nyakatura EK, Fels JM, Ng M, Mittler E, Pan J, Bharrhan S, Wec AZ, Lai JR, Sidhu SS, Tischler ND, Rey FA, Moffat J, Brummelkamp TR, Wang Z, Dye JM, Chandran K. Nature. 2018 Nov;563(7732):559-563. doi: 10.1038/s41586-018-0702-1. Epub 2018 Nov 21. PubMed |
| NLRX1 Negatively Regulates Group A Streptococcus Invasion and Autophagy Induction by Interacting With the Beclin 1-UVRAG Complex. Aikawa C, Nakajima S, Karimine M, Nozawa T, Minowa-Nozawa A, Toh H, Yamada S, Nakagawa I. Front Cell Infect Microbiol. 2018 Nov 14;8:403. doi: 10.3389/fcimb.2018.00403. eCollection 2018. PubMed |
| NKG2A Blockade Potentiates CD8 T Cell Immunity Induced by Cancer Vaccines. van Montfoort N, Borst L, Korrer MJ, Sluijter M, Marijt KA, Santegoets SJ, van Ham VJ, Ehsan I, Charoentong P, Andre P, Wagtmann N, Welters MJP, Kim YJ, Piersma SJ, van der Burg SH, van Hall T. Cell. 2018 Dec 13;175(7):1744-1755.e15. doi: 10.1016/j.cell.2018.10.028. Epub 2018 Nov 29. PubMed |
| Mapping of a responsible region for sex reversal upstream of Sox9 by production of mice with serial deletion in a genomic locus. Ogawa Y, Terao M, Hara S, Tamano M, Okayasu H, Kato T, Takada S. Sci Rep. 2018 Nov 30;8(1):17514. doi: 10.1038/s41598-018-35746-0. PubMed |
| Interleukin 34 (IL-34) cell-surface localization regulated by the molecular chaperone 78-kDa glucose-regulated protein facilitates the differentiation of monocytic cells. Ogawa S, Matsuoka Y, Takada M, Matsui K, Yamane F, Kubota E, Yasuhara S, Hieda K, Kanayama N, Hatano N, Tokumitsu H, Magari M. J Biol Chem. 2019 Feb 15;294(7):2386-2396. doi: 10.1074/jbc.RA118.006226. Epub 2018 Dec 20. PubMed |
| Homology-independent multiallelic disruption via CRISPR/Cas9-based knock-in yields distinct functional outcomes in human cells. Zhang C, He X, Kwok YK, Wang F, Xue J, Zhao H, Suen KW, Wang CC, Ren J, Chen GG, Lai PBS, Li J, Xia Y, Chan AM, Chan WY, Feng B. BMC Biol. 2018 Dec 28;16(1):151. doi: 10.1186/s12915-018-0616-2. PubMed |
| Lysosomal Signaling Licenses Embryonic Stem Cell Differentiation via Inactivation of Tfe3. Villegas F, Lehalle D, Mayer D, Rittirsch M, Stadler MB, Zinner M, Olivieri D, Vabres P, Duplomb-Jego L, De Bont ESJM, Duffourd Y, Duijkers F, Avila M, Genevieve D, Houcinat N, Jouan T, Kuentz P, Lichtenbelt KD, Thauvin-Robinet C, St-Onge J, Thevenon J, van Gassen KLI, van Haelst M, van Koningsbruggen S, Hess D, Smallwood SA, Riviere JB, Faivre L, Betschinger J. Cell Stem Cell. 2019 Feb 7;24(2):257-270.e8. doi: 10.1016/j.stem.2018.11.021. Epub 2018 Dec 27. PubMed |
| Novel Chimeric Gene Therapy Vectors Based on Adeno-Associated Virus and Four Different Mammalian Bocaviruses. Fakhiri J, Schneider MA, Puschhof J, Stanifer M, Schildgen V, Holderbach S, Voss Y, El Andari J, Schildgen O, Boulant S, Meister M, Clevers H, Yan Z, Qiu J, Grimm D. Mol Ther Methods Clin Dev. 2019 Jan 18;12:202-222. doi: 10.1016/j.omtm.2019.01.003. eCollection 2019 Mar 15. PubMed |
Reducible branched ester-amine quadpolymers (rBEAQs) co-delivering plasmid DNA and RNA oligonucleotides enable CRISPR/Cas9 genome editing.
Rui Y, Wilson D, Sanders K, Green JJ.
ACS Appl Mater Interfaces. 2019 Feb 22. doi: 10.1021/acsami.8b20206.
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| SARM1 deficiency up-regulates XAF1, promotes neuronal apoptosis, and accelerates prion disease. Zhu C, Li B, Frontzek K, Liu Y, Aguzzi A. J Exp Med. 2019 Apr 1;216(4):743-756. doi: 10.1084/jem.20171885. Epub 2019 Mar 6. PubMed |
| CRISPR-induced RASGAP deficiencies in colorectal cancer organoids reveal that only loss of NF1 promotes resistance to EGFR inhibition. Post JB, Hami N, Mertens AEE, Elfrink S, Bos JL, Snippert HJG. Oncotarget. 2019 Feb 15;10(14):1440-1457. doi: 10.18632/oncotarget.26677. eCollection 2019 Feb 15. PubMed |
| The Chromatin Structure of CRISPR-Cas9 Target DNA Controls the Balance between Mutagenic and Homology-Directed Gene-Editing Events. Janssen JM, Chen X, Liu J, Goncalves MAFV. Mol Ther Nucleic Acids. 2019 Jun 7;16:141-154. doi: 10.1016/j.omtn.2019.02.009. Epub 2019 Feb 20. PubMed |
| A critical regulator of Bcl2 revealed by systematic transcript discovery of lncRNAs associated with T-cell differentiation. Saadi W, Kermezli Y, Dao LTM, Mathieu E, Santiago-Algarra D, Manosalva I, Torres M, Belhocine M, Pradel L, Loriod B, Aribi M, Puthier D, Spicuglia S. Sci Rep. 2019 Mar 18;9(1):4707. doi: 10.1038/s41598-019-41247-5. PubMed |
| TMEPAI/PMEPA1 inhibits Wnt signaling by regulating beta-catenin stability and nuclear accumulation in triple negative breast cancer cells. Amalia R, Abdelaziz M, Puteri MU, Hwang J, Anwar F, Watanabe Y, Kato M. Cell Signal. 2019 Jul;59:24-33. doi: 10.1016/j.cellsig.2019.03.016. Epub 2019 Mar 16. PubMed |
| Evolution and Comprehensive Analysis of DNaseI Hypersensitive Sites in Regulatory Regions of Primate Brain-Related Genes. Lu Y, Wang X, Yu H, Li J, Jiang Z, Chen B, Lu Y, Wang W, Han C, Ouyang Y, Huang L, Chen C, Tian W, Ling F. Front Genet. 2019 Mar 7;10:152. doi: 10.3389/fgene.2019.00152. eCollection 2019. PubMed |
| Multiplexed and tunable transcriptional activation by promoter insertion using nuclease-assisted vector integration. Brown A, Winter J, Gapinske M, Tague N, Woods WS, Perez-Pinera P. Nucleic Acids Res. 2019 Jul 9;47(12):e67. doi: 10.1093/nar/gkz210. PubMed |
PCNA-Mediated Degradation of p21 Coordinates the DNA Damage Response and Cell Cycle Regulation in Individual Cells.
Sheng C, Mendler IH, Rieke S, Snyder P, Jentsch M, Friedrich D, Drossel B, Loewer A.
Cell Rep. 2019 Apr 2;27(1):48-58.e7. doi: 10.1016/j.celrep.2019.03.031.
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| DNA Damage Signaling-Induced Cancer Cell Reprogramming as a Driver of Tumor Relapse. Filipponi D, Emelyanov A, Muller J, Molina C, Nichols J, Bulavin DV. Mol Cell. 2019 May 16;74(4):651-663.e8. doi: 10.1016/j.molcel.2019.03.002. Epub 2019 Apr 3. PubMed |
| Crumbs3 is a critical factor that regulates invasion and metastasis of colon adenocarcinoma via the specific interaction with FGFR1. Iioka H, Saito K, Sakaguchi M, Tachibana T, Homma K, Kondo E. Int J Cancer. 2019 Apr 13. doi: 10.1002/ijc.32336. PubMed |
| Comparison of gene editing efficiencies of CRISPR/Cas9 and TALEN for generation of MSTN knock-out cashmere goats. Zhang J, Liu J, Yang W, Cui M, Dai B, Dong Y, Yang J, Zhang X, Liu D, Liang H, Cang M. Theriogenology. 2019 Jul 1;132:1-11. doi: 10.1016/j.theriogenology.2019.03.029. Epub 2019 Apr 1. PubMed |
| CEP120 interacts with C2CD3 and Talpid3 and is required for centriole appendage assembly and ciliogenesis. Tsai JJ, Hsu WB, Liu JH, Chang CW, Tang TK. Sci Rep. 2019 Apr 15;9(1):6037. doi: 10.1038/s41598-019-42577-0. PubMed |
| Increasing the specificity of CRISPR systems with engineered RNA secondary structures. Kocak DD, Josephs EA, Bhandarkar V, Adkar SS, Kwon JB, Gersbach CA. Nat Biotechnol. 2019 Jun;37(6):657-666. doi: 10.1038/s41587-019-0095-1. Epub 2019 Apr 15. PubMed |
| Distinct Requirements for HIV-1 Accessory Proteins during Cell Coculture and Cell-Free Infection. Zotova A, Atemasova A, Pichugin A, Filatov A, Mazurov D. Viruses. 2019 Apr 26;11(5). pii: v11050390. doi: 10.3390/v11050390. PubMed |
| Genome Editing in Mammalian Cell Lines using CRISPR-Cas. Liu KI, Sutrisnoh NB, Wang Y, Tan MH. J Vis Exp. 2019 Apr 11;(146). doi: 10.3791/59086. PubMed |
| Targeted deletion of BCL11A gene by CRISPR-Cas9 system for fetal hemoglobin reactivation: A promising approach for gene therapy of beta thalassemia disease. Khosravi MA, Abbasalipour M, Concordet JP, Berg JV, Zeinali S, Arashkia A, Azadmanesh K, Buch T, Karimipoor M. Eur J Pharmacol. 2019 Jul 5;854:398-405. doi: 10.1016/j.ejphar.2019.04.042. Epub 2019 Apr 27. PubMed |
| Multiplexed promoterless gene expression with CRISPReader. Zhan H, Zhou Q, Gao Q, Li J, Huang W, Liu Y. Genome Biol. 2019 Jun 3;20(1):113. doi: 10.1186/s13059-019-1712-5. PubMed |
KLF4 protein stability regulated by interaction with pluripotency transcription factors overrides transcriptional control.
Dhaliwal NK, Abatti LE, Mitchell JA.
Genes Dev. 2019 Jun 20. pii: gad.324319.119. doi: 10.1101/gad.324319.119.
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| HSF2BP Interacts with a Conserved Domain of BRCA2 and Is Required for Mouse Spermatogenesis. Brandsma I, Sato K, van Rossum-Fikkert SE, van Vliet N, Sleddens E, Reuter M, Odijk H, van den Tempel N, Dekkers DHW, Bezstarosti K, Demmers JAA, Maas A, Lebbink J, Wyman C, Essers J, van Gent DC, Baarends WM, Knipscheer P, Kanaar R, Zelensky AN. Cell Rep. 2019 Jun 25;27(13):3790-3798.e7. doi: 10.1016/j.celrep.2019.05.096. PubMed |
| Adhesion G-protein coupled receptor 56 is required for 3T3-L1 adipogenesis. Al Hasan M, Roy P, Dolan S, Martin PE, Patterson S, Bartholomew C. J Cell Physiol. 2020 Feb;235(2):1601-1614. doi: 10.1002/jcp.29079. Epub 2019 Jul 15. PubMed |
| Stabilizing heterochromatin by DGCR8 alleviates senescence and osteoarthritis. Deng L, Ren R, Liu Z, Song M, Li J, Wu Z, Ren X, Fu L, Li W, Zhang W, Guillen P, Izpisua Belmonte JC, Chan P, Qu J, Liu GH. Nat Commun. 2019 Jul 26;10(1):3329. doi: 10.1038/s41467-019-10831-8. PubMed |
| Cholesterol-rich lipid-mediated nanoparticles boost of transfection efficiency, utilized for gene editing by CRISPR-Cas9. Hosseini ES, Nikkhah M, Hosseinkhani S. Int J Nanomedicine. 2019 Jun 11;14:4353-4366. doi: 10.2147/IJN.S199104. eCollection 2019. PubMed |
| A Cancer-Associated Missense Mutation in PP2A-Aalpha Increases Centrosome Clustering during Mitosis. Antao NV, Marcet-Ortega M, Cifani P, Kentsis A, Foley EA. iScience. 2019 Sep 27;19:74-82. doi: 10.1016/j.isci.2019.07.018. Epub 2019 Jul 17. PubMed |
| Opposing Functions of Interferon Coordinate Adaptive and Innate Immune Responses to Cancer Immune Checkpoint Blockade. Benci JL, Johnson LR, Choa R, Xu Y, Qiu J, Zhou Z, Xu B, Ye D, Nathanson KL, June CH, Wherry EJ, Zhang NR, Ishwaran H, Hellmann MD, Wolchok JD, Kambayashi T, Minn AJ. Cell. 2019 Aug 8;178(4):933-948.e14. doi: 10.1016/j.cell.2019.07.019. PubMed |
| Precise in vivo genome editing via single homology arm donor mediated intron-targeting gene integration for genetic disease correction. Suzuki K, Yamamoto M, Hernandez-Benitez R, Li Z, Wei C, Soligalla RD, Aizawa E, Hatanaka F, Kurita M, Reddy P, Ocampo A, Hishida T, Sakurai M, Nemeth AN, Nunez Delicado E, Campistol JM, Magistretti P, Guillen P, Rodriguez Esteban C, Gong J, Yuan Y, Gu Y, Liu GH, Lopez-Otin C, Wu J, Zhang K, Izpisua Belmonte JC. Cell Res. 2019 Oct;29(10):804-819. doi: 10.1038/s41422-019-0213-0. Epub 2019 Aug 23. PubMed |
Targeting of early endosomes by autophagy facilitates EGFR recycling and signalling.
Fraser J, Simpson J, Fontana R, Kishi-Itakura C, Ktistakis NT, Gammoh N.
EMBO Rep. 2019 Oct 4;20(10):e47734. doi: 10.15252/embr.201947734. Epub 2019 Aug 26.
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An In Vitro Human Segmentation Clock Model Derived from Embryonic Stem Cells.
Chu LF, Mamott D, Ni Z, Bacher R, Liu C, Swanson S, Kendziorski C, Stewart R, Thomson JA.
Cell Rep. 2019 Aug 27;28(9):2247-2255.e5. doi: 10.1016/j.celrep.2019.07.090.
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| Efficient generation of Knock-in/Knock-out marmoset embryo via CRISPR/Cas9 gene editing. Kumita W, Sato K, Suzuki Y, Kurotaki Y, Harada T, Zhou Y, Kishi N, Sato K, Aiba A, Sakakibara Y, Feng G, Okano H, Sasaki E. Sci Rep. 2019 Sep 3;9(1):12719. doi: 10.1038/s41598-019-49110-3. PubMed |
| Hepatitis C Virus Infection Is Inhibited by a Noncanonical Antiviral Signaling Pathway Targeted by NS3-NS4A. Vazquez C, Tan CY, Horner SM. J Virol. 2019 Nov 13;93(23). pii: JVI.00725-19. doi: 10.1128/JVI.00725-19. Print 2019 Dec 1. PubMed |
| Deletion of a Seminal Gene Cluster Reinforces a Crucial Role of SVS2 in Male Fertility. Shindo M, Inui M, Kang W, Tamano M, Tingwei C, Takada S, Hibino T, Yoshida M, Yoshida K, Okada H, Iwamoto T, Miyado K, Kawano N. Int J Mol Sci. 2019 Sep 14;20(18). pii: ijms20184557. doi: 10.3390/ijms20184557. PubMed |
| A new patient-derived iPSC model for dystroglycanopathies validates a compound that increases glycosylation of alpha-dystroglycan. Kim J, Lana B, Torelli S, Ryan D, Catapano F, Ala P, Luft C, Stevens E, Konstantinidis E, Louzada S, Fu B, Paredes-Redondo A, Chan AE, Yang F, Stemple DL, Liu P, Ketteler R, Selwood DL, Muntoni F, Lin YY. EMBO Rep. 2019 Nov 5;20(11):e47967. doi: 10.15252/embr.201947967. Epub 2019 Sep 30. PubMed |
| Angiogenin generates specific stress-induced tRNA halves and is not involved in tRF-3-mediated gene silencing. Su Z, Kuscu C, Malik A, Shibata E, Dutta A. J Biol Chem. 2019 Nov 8;294(45):16930-16941. doi: 10.1074/jbc.RA119.009272. Epub 2019 Oct 3. PubMed |
| Coordinated removal of repressive epigenetic modifications during induced reversal of cell identity. Tran KA, Dillingham CM, Sridharan R. EMBO J. 2019 Nov 15;38(22):e101681. doi: 10.15252/embj.2019101681. Epub 2019 Oct 4. PubMed |
| Functional analysis and development of a CRISPR/Cas9 allelic series for a CPR5 ortholog necessary for proper growth of soybean trichomes. Campbell BW, Hoyle JW, Bucciarelli B, Stec AO, Samac DA, Parrott WA, Stupar RM. Sci Rep. 2019 Oct 14;9(1):14757. doi: 10.1038/s41598-019-51240-7. PubMed |
| A Maternal Transcription Factor, Junction Mediating and Regulatory Protein is Required for Preimplantation Development in the Mouse. Lin ZL, Li YH, Jin YX, Kim NH. Dev Reprod. 2019 Sep;23(3):285-295. doi: 10.12717/DR.2019.23.3.285. Epub 2019 Sep 30. PubMed |
| CRISPR/Cas9-mediated gene correction in hemophilia B patient-derived iPSCs. Morishige S, Mizuno S, Ozawa H, Nakamura T, Mazahery A, Nomura K, Seki R, Mouri F, Osaki K, Yamamura K, Okamura T, Nagafuji K. Int J Hematol. 2020 Feb;111(2):225-233. doi: 10.1007/s12185-019-02765-0. Epub 2019 Oct 29. PubMed |
| LDAF1 and Seipin Form a Lipid Droplet Assembly Complex. Chung J, Wu X, Lambert TJ, Lai ZW, Walther TC, Farese RV Jr. Dev Cell. 2019 Dec 2;51(5):551-563.e7. doi: 10.1016/j.devcel.2019.10.006. Epub 2019 Nov 7. PubMed |
| Novel gRNA design pipeline to develop broad-spectrum CRISPR/Cas9 gRNAs for safe targeting of the HIV-1 quasispecies in patients. Sullivan NT, Dampier W, Chung CH, Allen AG, Atkins A, Pirrone V, Homan G, Passic S, Williams J, Zhong W, Kercher K, Desimone M, Li L, C Antell G, Mell JC, Ehrlich GD, Szep Z, Jacobson JM, Nonnemacher MR, Wigdahl B. Sci Rep. 2019 Nov 19;9(1):17088. doi: 10.1038/s41598-019-52353-9. PubMed |
| PODXL1 promotes metastasis of the pancreatic ductal adenocarcinoma by activating the C5aR/C5a axis from the tumor microenvironment. Saito K, Iioka H, Maruyama S, Sumardika IW, Sakaguchi M, Kondo E. Neoplasia. 2019 Dec;21(12):1121-1132. doi: 10.1016/j.neo.2019.09.003. Epub 2019 Nov 20. PubMed |
| Zfp281 orchestrates interconversion of pluripotent states by engaging Ehmt1 and Zic2. Mayer D, Stadler MB, Rittirsch M, Hess D, Lukonin I, Winzi M, Smith A, Buchholz F, Betschinger J. EMBO J. 2020 Jan 15;39(2):e102591. doi: 10.15252/embj.2019102591. Epub 2019 Nov 29. PubMed |
| Loss of IL-10 signaling in macrophages limits bacterial killing driven by prostaglandin E2. Mukhopadhyay S, Heinz E, Porreca I, Alasoo K, Yeung A, Yang HT, Schwerd T, Forbester JL, Hale C, Agu CA, Choi YH, Rodrigues J, Capitani M, Jostins-Dean L, Thomas DC, Travis S, Gaffney D, Skarnes WC, Thomson N, Uhlig HH, Dougan G, Powrie F. J Exp Med. 2020 Feb 3;217(2). pii: 132614. doi: 10.1084/jem.20180649. PubMed |
| Forced isoform switching of Neat1_1 to Neat1_2 leads to the loss of Neat1_1 and the hyperformation of paraspeckles but does not affect the development and growth of mice. Isobe M, Toya H, Mito M, Chiba T, Asahara H, Hirose T, Nakagawa S. RNA. 2020 Mar;26(3):251-264. doi: 10.1261/rna.072587.119. Epub 2019 Dec 10. PubMed |
| Characterization of IRE1alpha in Neuro2a cells by pharmacological and CRISPR/Cas9 approaches. Oh-Hashi K, Kohno H, Kandeel M, Hirata Y. Mol Cell Biochem. 2020 Feb;465(1-2):53-64. doi: 10.1007/s11010-019-03666-w. Epub 2019 Dec 13. PubMed |
| Group A Streptococcus NAD-Glycohydrolase Inhibits Caveolin 1-Mediated Internalization Into Human Epithelial Cells. Toh H, Lin CY, Nakajima S, Aikawa C, Nozawa T, Nakagawa I. Front Cell Infect Microbiol. 2019 Nov 28;9:398. doi: 10.3389/fcimb.2019.00398. eCollection 2019. PubMed |
| NEDD4 and NEDD4L regulate Wnt signalling and intestinal stem cell priming by degrading LGR5 receptor. Novellasdemunt L, Kucharska A, Jamieson C, Prange-Barczynska M, Baulies A, Antas P, van der Vaart J, Gehart H, Maurice MM, Li VS. EMBO J. 2020 Feb 3;39(3):e102771. doi: 10.15252/embj.2019102771. Epub 2019 Dec 23. PubMed |
| Intra-Vkappa Cluster Recombination Shapes the Ig Kappa Locus Repertoire. Shinoda K, Maman Y, Canela A, Schatz DG, Livak F, Nussenzweig A. Cell Rep. 2019 Dec 24;29(13):4471-4481.e6. doi: 10.1016/j.celrep.2019.11.088. PubMed |
| Efficient production and transmission of CRISPR/Cas9-mediated mutant alleles at the IG-DMR via generation of mosaic mice using a modified 2CC method. Hara S, Terao M, Muramatsu A, Takada S. Sci Rep. 2019 Dec 27;9(1):20202. doi: 10.1038/s41598-019-56676-5. PubMed |
| Degradation of the Tumor Suppressor PDCD4 Is Impaired by the Suppression of p62/SQSTM1 and Autophagy. Manirujjaman M, Ozaki I, Murata Y, Guo J, Xia J, Nishioka K, Perveen R, Takahashi H, Anzai K, Matsuhashi S. Cells. 2020 Jan 15;9(1). pii: cells9010218. doi: 10.3390/cells9010218. PubMed |
| Long-term development of human iPSC-derived pyramidal neurons quantified after transplantation into the neonatal mouse cortex. D'Alessio R, Koukouli F, Blanchard S, Catteau J, Rais C, Lemonnier T, Feraud O, Bennaceur-Griscelli A, Groszer M, Maskos U. Dev Biol. 2020 May 1;461(1):86-95. doi: 10.1016/j.ydbio.2020.01.009. Epub 2020 Jan 23. PubMed |
| Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors. Choi IY, Lim H, Cho HJ, Oh Y, Chou BK, Bai H, Cheng L, Kim YJ, Hyun S, Kim H, Shin JH, Lee G. Elife. 2020 Feb 3;9. pii: 46981. doi: 10.7554/eLife.46981. PubMed |
| Effects of TDP2/VPg Unlinkase Activity on Picornavirus Infections Downstream of Virus Translation. Holmes AC, Zagnoli-Vieira G, Caldecott KW, Semler BL. Viruses. 2020 Jan 31;12(2). pii: v12020166. doi: 10.3390/v12020166. PubMed |
| TBC1D9 regulates TBK1 activation through Ca(2+) signaling in selective autophagy. Nozawa T, Sano S, Minowa-Nozawa A, Toh H, Nakajima S, Murase K, Aikawa C, Nakagawa I. Nat Commun. 2020 Feb 7;11(1):770. doi: 10.1038/s41467-020-14533-4. PubMed |
| IRF5 Promotes Influenza Virus-Induced Inflammatory Responses in Human Induced Pluripotent Stem Cell-Derived Myeloid Cells and Murine Models. Forbester JL, Clement M, Wellington D, Yeung A, Dimonte S, Marsden M, Chapman L, Coomber EL, Tolley C, Lees E, Hale C, Clare S, Udalova I, Dong T, Dougan G, Humphreys IR. J Virol. 2020 Apr 16;94(9). pii: JVI.00121-20. doi: 10.1128/JVI.00121-20. Print 2020 Apr 16. PubMed |
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Find and cut-and-transfer (FiCAT) mammalian genome engineering.
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