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Addgene

pU6_gRNA_handle_U6t
(Plasmid #49016)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49016 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    phi-Yellow-Dest
  • Backbone manufacturer
    Evrogen
  • Backbone size (bp) 4000
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6_gRNA_handle_U6t was a gift from Timothy Lu (Addgene plasmid # 49016 ; http://n2t.net/addgene:49016 ; RRID:Addgene_49016)
  • For your References section:

    Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. Farzadfard F, Perli SD, Lu TK. ACS Synth Biol. 2013 Sep 11. 10.1021/sb400081r PubMed 23977949