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PurposeExpresses Cas9 fusion to human CtIP protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 109403 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonehCas9 Addgene ID 41815
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Backbone manufacturerChurch lab
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCtIP
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Alt nameRBBP8
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SpeciesH. sapiens (human)
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Insert Size (bp)2694
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Mutationfull length CtIP
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Entrez GeneRBBP8 (a.k.a. COM1, CTIP, JAWAD, JWDS, RIM, SAE2, SCKL2)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCas9-CtIP was a gift from Jean-Paul Concordet (Addgene plasmid # 109403 ; http://n2t.net/addgene:109403 ; RRID:Addgene_109403) -
For your References section:
CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair. Charpentier M, Khedher AHY, Menoret S, Brion A, Lamribet K, Dardillac E, Boix C, Perrouault L, Tesson L, Geny S, De Cian A, Itier JM, Anegon I, Lopez B, Giovannangeli C, Concordet JP. Nat Commun. 2018 Mar 19;9(1):1133. doi: 10.1038/s41467-018-03475-7. 10.1038/s41467-018-03475-7 PubMed 29556040