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Addgene

HA-PKB-pcDNA3-K179A
(Plasmid #24652)

Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 24652 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKB
  • Mutation
    K179A
  • Entrez Gene
    AKT1 (a.k.a. BOS_20453)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Two additional mutations (N2D and D149E) detected in Addgene quality control sequencing. Author reports that these mutations do not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-PKB-pcDNA3-K179A was a gift from Jim Woodgett (Addgene plasmid # 24652 ; http://n2t.net/addgene:24652 ; RRID:Addgene_24652)