FUW-tetO-hOKMminiSOX
(Plasmid #206383)

• Purpose: Expresses four genes (human OCT4, KLF4, c-MYC and mouse miniSOX) in mammalian cells, for lentivirus generation.
• Depositing Lab: Ralf Jauch
• Publication: Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597. doi: 10.1093/nar/gkad597.

Backbone

• Vector backbone: FUW-tetO
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OCT4, KLF4, c-MYC, miniSOX
• Alt name: OCT4-T2A-KLF4-P2A-c-MYC-E2A-miniSOX[mouse]
• Species: H. sapiens (human), M. musculus (mouse)
• Entrez Gene: POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
• Entrez Gene: KLF4 (a.k.a. EZF, GKLF)
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry