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Addgene

PB-TRE-OKSM
(Plasmid #204498)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204498 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUC19
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    OCT4
  • Species
    H. sapiens (human)
  • Entrez Gene
    POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)

Gene/Insert 2

  • Gene/Insert name
    KLF4
  • Species
    H. sapiens (human)
  • Entrez Gene
    KLF4 (a.k.a. EZF, GKLF)

Gene/Insert 3

  • Gene/Insert name
    SOX2
  • Species
    H. sapiens (human)
  • Entrez Gene
    SOX2 (a.k.a. ANOP3, MCOPS3)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    OKSM fragment was obtained from pMaster3 (Addgene plasmid #58526) (Wu, Wu, Zhang, & Capecchi, PNAS, 2014), with the modification of Klf4 according to a previous report (Kim et al., Stem Cell Reports, 2015)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-TRE-OKSM was a gift from Wataru Fujii (Addgene plasmid # 204498 ; http://n2t.net/addgene:204498 ; RRID:Addgene_204498)
  • For your References section:

    Establishment of African pygmy mouse induced pluripotent stem cells using defined doxycycline inducible transcription factors. Matsuya S, Fujino K, Imai H, Kusakabe KT, Fujii W, Kano K. Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.1038/s41598-024-53687-9. 10.1038/s41598-024-53687-9 PubMed 38331995