PB-TRE-OKSM
(Plasmid
#204498)
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PurposeExpresses Oct4, Klf4, Sox2 and c-Myc by tet-on promoter in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 204498 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameOCT4
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SpeciesH. sapiens (human)
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Entrez GenePOU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
Gene/Insert 2
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Gene/Insert nameKLF4
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SpeciesH. sapiens (human)
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Entrez GeneKLF4 (a.k.a. EZF, GKLF)
Gene/Insert 3
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Gene/Insert nameSOX2
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SpeciesH. sapiens (human)
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Entrez GeneSOX2 (a.k.a. ANOP3, MCOPS3)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byOKSM fragment was obtained from pMaster3 (Addgene plasmid #58526) (Wu, Wu, Zhang, & Capecchi, PNAS, 2014), with the modification of Klf4 according to a previous report (Kim et al., Stem Cell Reports, 2015)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-TRE-OKSM was a gift from Wataru Fujii (Addgene plasmid # 204498 ; http://n2t.net/addgene:204498 ; RRID:Addgene_204498) -
For your References section:
Establishment of African pygmy mouse induced pluripotent stem cells using defined doxycycline inducible transcription factors. Matsuya S, Fujino K, Imai H, Kusakabe KT, Fujii W, Kano K. Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.1038/s41598-024-53687-9. 10.1038/s41598-024-53687-9 PubMed 38331995