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Addgene

GSK3beta/pET29b
(Plasmid #16315)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16315 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29b
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5370
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GSK3beta
  • Alt name
    GSK3b
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1200
  • Entrez Gene
    gsk3b.L (a.k.a. XELAEV_180112401mg, gsk-3, gsk3, gsk3-beta, gsk3b, gsk3beta, xgsk-3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer T7 promoter primer
  • 3′ sequencing primer T7 terminator primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GSK3beta/pET29b was a gift from Peter Klein (Addgene plasmid # 16315 ; http://n2t.net/addgene:16315 ; RRID:Addgene_16315)

  • For your References section:

    Activation of the Wnt signaling pathway: a molecular mechanism for lithium action. Hedgepeth CM, Conrad LJ, Zhang J, Huang HC, Lee VM, Klein PS. Dev Biol. 1997 May 1. 185(1):82-91. 10.1006/dbio.1997.8552 PubMed 9169052
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