HA GSK3 beta S9A pcDNA3
(Plasmid #14754)

• Purpose: Mammalian expression of human GSK3 beta constitutively active mutant with HA tag
• Depositing Lab: Jim Woodgett
• Publication: Stambolic et al Biochem J. 1994 Nov 1. 303 ( Pt 3)():701-4.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GSK3 beta S9A
• Alt name: GSK3b
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• Mutation: S9A. Constitutively active mutant.
• Entrez Gene: GSK3B
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 61 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

S2A in the sequence does not affect function, used to introduce restriction enzyme site