Htb2-GFP
(Plasmid
#117669)
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PurposeColorize the nuclei
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 117669 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneUnknown
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHtb2-GFP
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MutationContains the gene encoding the Histone Htb2 from S.cerevisiae tagged with a GFP at the C-terminal. The protein is expressed constituvely by the promoter pGAP.
- Promoter pGAP
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Integration plasmid in the pGAP locus. Please note that there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied Genbank sequence. The authors have noted that the discrepancies do NOT affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Htb2-GFP was a gift from Pau Ferrer & Benjamin Glick (Addgene plasmid # 117669 ; http://n2t.net/addgene:117669 ; RRID:Addgene_117669) -
For your References section:
An improved secretion signal enhances the secretion of model proteins from Pichia pastoris. Barrero JJ, Casler JC, Valero F, Ferrer P, Glick BS. Microb Cell Fact. 2018 Oct 12;17(1):161. doi: 10.1186/s12934-018-1009-5. 10.1186/s12934-018-1009-5 PubMed 30314480
