MoClo CRISPR/Cas Toolkit for Plants
(Kit #
1000000159
)
Depositing Lab: Vladimir Nekrasov
The MoClo CRISPR/Cas Toolkit for Plants includes 95 plasmids consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and dicots.
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publication
A Modular Cloning Toolkit for Genome Editing in Plants Hahn F, Korolev A, Loures LS, Nekrasov V. BMC Plant Biology. 2020 Apr 23, 20(1):179; doi: 10.1186/s12870-020-02388-2. PubMed PMID: 32326901.
Description
Included in the MoClo CRISPR/Cas Toolkit for Plants are 95 plasmids to generate CRISPR constructs for genome editing in monocots and dicots. A variety of nucleases including SpCas9, SaCas9, FnCas12a, LbCas12a, xCas9 3.7, and SpCas9-NG allow for targeting of different PAM sites within the plant genome. Plasmids encoding both cytosine and adenine base editors are also included. Expression of these factors can be driven by Pol II promoters for use in both monocots and dicots. For expression of targeting sequences used by these different nucleases, this kit also includes gRNA, crRNA, and tRNA-gRNA backbones as well as Pol III promoters. Extensive protocols can be found in the original publication for the construction of plasmids for use in a number of different experimental systems.
See Kit Documentation under the Protocols & Resources tab for a complete list of the plasmids and inserts.
CRISPR/Cas constructs for genome engineering in monocots and dicots. A) Construct with a single sgRNA. B) Four sgRNAs in a single construct. Figure from Hahn et al. BMC Plant Biology (2020) 20:179 https://doi.org/10.1186/s12870-020-02388-2 licensed under a Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).
Kit Documentation
Kit 1000000159 Plasmids 24.8Â KBHow to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The MoClo CRISPR/Cas Toolkit for Plants was a gift from Vladimir Nekrasov (Addgene kit #1000000159).”
For your Reference section:
A Modular Cloning Toolkit for Genome Editing in Plants Hahn F, Korolev A, Loures LS, Nekrasov V. BMC Plant Biology. 2020 Apr 23, 20(1):179; doi: 10.1186/s12870-020-02388-2. PubMed PMID: 32326901.
MoClo CRISPR/Cas Toolkit for Plants - #1000000159
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Spectinomycin | |
| Kanamycin | |
| Ampicillin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | pFH13 | Spectinomycin |
| A / 2 | pFH14 | Spectinomycin |
| A / 3 | pFH15 | Spectinomycin |
| A / 4 | pFH16 | Spectinomycin |
| A / 5 | pFH17 | Spectinomycin |
| A / 6 | pFH22 | Spectinomycin |
| A / 7 | pFH32 | Spectinomycin |
| A / 8 | pFH45 | Spectinomycin |
| A / 9 | pFH46 | Spectinomycin |
| A / 10 | pFH47 | Spectinomycin |
| A / 11 | pFH48 | Spectinomycin |
| A / 12 | pFH55 | Spectinomycin |
| B / 1 | pFH76 | Spectinomycin |
| B / 2 | pFH77 | Spectinomycin |
| B / 3 | pFH79 | Spectinomycin |
| B / 4 | pFH91 | Spectinomycin |
| B / 5 | pFH92 | Spectinomycin |
| B / 6 | pFH40 | Spectinomycin |
| B / 7 | pFH41 | Spectinomycin |
| B / 8 | pFH42 | Spectinomycin |
| B / 9 | pFH56 | Spectinomycin |
| B / 10 | pFH57 | Spectinomycin |
| B / 11 | pFH31 | Spectinomycin |
| B / 12 | pFH33 | Spectinomycin |
| C / 1 | pFH34 | Spectinomycin |
| C / 2 | pFH35 | Spectinomycin |
| C / 3 | pFH36 | Spectinomycin |
| C / 4 | pFH37 | Spectinomycin |
| C / 5 | pFH38 | Spectinomycin |
| C / 6 | pFH39 | Spectinomycin |
| C / 7 | pFH43 | Spectinomycin |
| C / 8 | pFH44 | Spectinomycin |
| C / 9 | pFH24 | Spectinomycin |
| C / 10 | pFH25 | Spectinomycin |
| C / 11 | pAK-EL-01 | Kanamycin |
| C / 12 | pAK-EL-02 | Kanamycin |
| D / 1 | pAK-EL-03 | Kanamycin |
| D / 2 | pAK-EL-04 | Kanamycin |
| D / 3 | pAK-EL-05 | Kanamycin |
| D / 4 | pFH85 | Kanamycin |
| D / 5 | pFH86 | Kanamycin |
| D / 6 | pFH87 | Kanamycin |
| D / 7 | pFH88 | Kanamycin |
| D / 8 | pFH89 | Kanamycin |
| D / 9 | pFH90 | Kanamycin |
| D / 10 | pFH99 | Kanamycin |
| D / 11 | pFH100 | Kanamycin |
| D / 12 | pFH101 | Kanamycin |
| E / 1 | pFH102 | Kanamycin |
| E / 2 | pFH103 | Kanamycin |
| E / 3 | pFH104 | Kanamycin |
| E / 4 | pFH113 | Kanamycin |
| E / 5 | pFH49 | Kanamycin |
| E / 6 | pFH50 | Kanamycin |
| E / 7 | pFH51 | Kanamycin |
| E / 8 | pFH71 | Kanamycin |
| E / 9 | pAK002 | Kanamycin |
| E / 10 | pAK003 | Kanamycin |
| E / 11 | pAK004 | Kanamycin |
| E / 12 | pAK005 | Kanamycin |
| F / 1 | pAK006 | Kanamycin |
| F / 2 | pAK007 | Kanamycin |
| F / 3 | pFH72 | Kanamycin |
| F / 4 | pFH73 | Kanamycin |
| F / 5 | pFH74 | Kanamycin |
| F / 6 | pFH75 | Kanamycin |
| F / 7 | pAK008 | Kanamycin |
| F / 8 | pAK009 | Kanamycin |
| F / 9 | pAK010 | Kanamycin |
| F / 10 | pAK011 | Kanamycin |
| F / 11 | pAK012 | Kanamycin |
| F / 12 | pFH130 | Kanamycin |
| G / 1 | pFH131 | Kanamycin |
| G / 2 | pFH23 | Ampicillin |
| G / 3 | pFH52 | Ampicillin |
| G / 4 | pFH53 | Ampicillin |
| G / 5 | pFH54 | Ampicillin |
| G / 6 | pFH58 | Ampicillin |
| G / 7 | pFH59 | Ampicillin |
| G / 8 | pFH60 | Ampicillin |
| G / 9 | pFH61 | Ampicillin |
| G / 10 | pFH68 | Ampicillin |
| G / 11 | pFH69 | Ampicillin |
| G / 12 | pFH70 | Ampicillin |
| H / 1 | pFH80 | Ampicillin |
| H / 2 | pFH81 | Ampicillin |
| H / 3 | pFH82 | Ampicillin |
| H / 4 | pFH84 | Ampicillin |
| H / 5 | pFH93 | Ampicillin |
| H / 6 | pFH109 | Ampicillin |
| H / 7 | pFH114 | Ampicillin |
| H / 8 | pFH132 | Ampicillin |
| H / 9 | pFH133 | Ampicillin |
| H / 10 | pFH66 | Ampicillin |
| H / 11 | pFH67 | Ampicillin |