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  2. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...GraviTrap columns, Cytiva 28-9852-55 or rProtein A GraviTrap columns, Cytiva 28-9852-54 LabMate PD10 Buffer...channel pipette Reagents Aspirating pipette, VWR 414004-265 5 mL pipette, VWR 89130-896 10 mL pipette, VWR...single channel pipette 0.5–10 µL single channel pipette 20–200 µL single channel pipette 2–20 µL single...VWR 89130-898 25 mL pipette, VWR 89130-900 50 mL pipette, VWR 89130-902 50 mL conical tubes, VWR 89039-...A2 Biological Safety Cabinet 4 °C Refrigerator Pipette controller Benchtop centrifuge compatible with ...tubes, VWR 76289-498 Before Starting Wipe down all pipettes and equipment with 10% bleach prior to use. Warm...the remaining space in the column with PBS and pipette several times to mix. Centrifuge at 3100 x g for...

  3. Protocol - How to Streak a Plate

    Type
    Protocol
    ...streaking it onto an LB agar plate. Last Update: Feb. 28, 2017 Protocol Video Watch the protocol video below...stab or glycerol stock . Using a sterile loop, pipette tip or toothpick, touch the bacteria growing within...plate, to create streak #2. Using a third sterile pipette tip, toothpick, or sterilized loop, drag through...

  4. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...sequences. Hum Gene Ther Methods. 2012 Feb;23(1):18-28. (Link opens in a new window) PMID: 22428977...Heating plate Pipettors 1–10 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...single channel pipette 1–10 µL multichannel pipette 2–50 µL multichannel pipette 20–200 µL multichannel...multichannel pipet to minimize pipetting error and variability Mix samples very well by pipetting back and...multichannel pipette Reagents Universal SYBR Master Mix 2X Primer pair targeting AAV2 ITR ( Aurnhammer et al., ...water Microcentrifuge tubes 96-well optical plate Pipette tips General Considerations Always run standards...that result from a variety of causes, such as pipetting error and sample evaporation. Make the master ...
Showing: 1 - 4 of 4 results