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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 13 results for: nif

Showing: 1 - 13 of 13 results

  1. Using a Light Microscope Protocol

    Type
    Protocol
    ...10x magnification. To determine the final magnification of your image, multiply the magnification of your...lens which magnifies the image, then the ocular lens, where the image is further magnified before it finally...for now) is the magnification power, such as 4x, 10x, or 20x. A 4x objective magnifies the image four ...10x objective magnifies it 10 times, and so on. The ocular lens also provides magnification and the power...most important properties of a microscope are magnification (the ability to make an image larger) and resolution...reaches your eyes. These lenses determine the magnification of the image and the resolution your microscope...performs essentially the same task but has different magnifying powers. When you look at the side of an objective...

  2. CRISPR Library Amplification

    Type
    Protocol
    ...for options if the recombinant band makes up a significant proportion of the DNA pool. Last Update: August...taking place during electroporation. Arcing would manifest as a loud pop often accompanied by some light ... off entirely if the column or reagents are significantly overloaded. If you find that your pellet outstrips...

  3. DNA Quantification

    Type
    Protocol
    ...the NanoDrop, if two consecutive samples have significantly different concentrations, it is possible that...between samples if they are expected to vary significantly in concentration. DNA dissolved in water is ...

  4. Gibson Assembly Protocol

    Type
    Protocol
    ...homology region. Hairpins in this region can significantly reduce the efficiency of two homologous ends...gel to check for size and yield. If there are significant amounts of undesired product, gel-purify DNA ...

  6. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...recipient plasmid + insert plate should have significantly more colonies than the recipient plasmid alone...recipient plasmid self-ligation, you will see significantly more colonies when you add ligase. If you do...

  10. Centrifugation

    Type
    Protocol
    ...volume as your samples. If your samples are significantly higher density than water, then you may need...
Showing: 1 - 13 of 13 results