...lab procedure for separating DNA by size (e.g., length in base pairs) for visualization and purification...longer ones. Thus, you can determine the approximate length of a DNA fragment by running it on an agarose gel...ladder (a collection of DNA fragments of known lengths). Last Update: Feb. 20, 2018 Protocol Video Watch... be purifying the DNA for later use, use long-wavelength UV and expose for as little time as possible ...

...absorb different wavelengths of light to varying degrees and most have a specific wavelength that they maximally...based on its absorbance, you need to know the wavelength of light that your substance maximally absorbs...

... (Gibson et al. , 2009). Regardless of fragment length or end compatibility, multiple overlapping DNA ...fragments at a time. Resources and References New England Biolabs (NEB) (Link opens in a new window) sells... window) . Gibson Assembly® is licensed to New England Biolabs by TelesisBio. Gibson DG, Young L, Chuang...

...gel electrophoresis, which separates DNA by their length in base pairs. Following electrophoresis, you can...cutting out the bands and you will want to use long-wavelength UV for as short a time as possible to get the...

...used for ligation, use only long-wavelength UV light. Short wavelength UV light will increase the chance...Materials Material Vendor and catalog # AgeI New England Biolabs (NEB) #R0552S EcoRI NEB #R0101S NEB buffer...

... DNA polymerase can create overhangs of varying length (typically 10-12 bp) based on a specific sequence...or tag sequences (where appropriate). The primer length is dependent on the T4 Pol "chew back" reaction...

...the amount of DNA used in a ligation based on the length of the DNA to get a proper ratio of 3 available...found on the web. Just enter the concentration, lengths of your insert and vector, and what ratio you want...

...cells. The problem is that the only version of full-length cDNA you can find for YGOI is in a bacterial expression...enzyme buffer (see (Link opens in a new window) New England Biolabs for more information about restriction ...

...should generally have the following properties: Length of 18-24 bases 40-60% G/C content Start and end...

.... to ligate an annealed oligo insert of 50bp in length into a 5kb vector, mix 100ng of the vector with...

...commercially available restriction enzymes, see New England Biolabs' website . Last Upload: May 18, 2018 Protocol...

...extension time can be adjusted according to the length of the target sequence. FAQ How do I design primers...

...cells for 13 days. lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described...

...temperatures, but you may need to increase your primer length and increase the Tm if you are trying to clone ...

...loading control. 1 lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described...

... until fully dissolved. Pro-Tip This step is challenging due to the high viscosity of PEG. Stirring under...

...Quality Control (QC) Pooled libraries can be challenging and expensive to ensure adequate quality, but...