...needed. Add stir bar and stir slowly at 4 °C for 1 h, then keep at 4 °C for 3 h without stirring to allow...controller Hazardous waste container pH meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex Reagents...PEI powder into 100 mL of deionized water. While stirring, slowly add hydrochloric acid until the solution...deionized water and adjust to a final volume of 1000 mL. Stir at RT until fully dissolved. Pro-Tip This step is... challenging due to the high viscosity of PEG. Stirring under medium heat will promote faster dissolution... to ~7.4. Autoclave or sterile filter. Pro-Tip Stirring during the cooling period is recommended or the...proceed overnight at 4 °C if needed. Transfer the entire sample to 3 x 500 mL conical bottles and centrifuge...

...deionized water in a 1 L bottle and stir on a magnetic stir plate. Stir until all particles have dissolved...platform set to 120 rpm 37 °C bead bath Vortex Stir bar Magnetic stir plate pH meter Reagents HEK293 cells Recombinant...presence of particles still in solution. Continue to stir until all particles have dissolved. This may take...

...wash and add RNase-free water or TE as soon as the entire tube is dried but while the white pellet is still...Pro-Tip To avoid multiple freeze-thaw cycles of your entire RNA sample, consider making smaller aliquots of...

...controller Hazardous waste container pH meter Stir plate Magnetic Stir Bar Reagents DMEM high glucose, Corning... of powder in 100 mL of deionized water. While stirring, slowly add hydrochloric acid until the solution...

...portion of the insert, but we do not sequence the entire plasmid. Addgene strongly recommends that you...Make sure the primer only anneals once within the entire construct. Is the primer an appropriate distance...

...amplified (the ORF in this case), not the Tm of the entire primer. If you are amplifying from a plasmid or...plenty of starting material. We recommend using your entire PCR reaction and 1μg of recipient plasmid. It is...

...linearize a plasmid to determine the size of the entire construct or excise some or all of an insert from...

...bottle may explode!) and then loosely cover the entire top of the bottle with aluminum foil. Autoclave...

...of powder into 100 mL of deionized water. While stirring, slowly add hydrochloric acid until the solution...

... science-fiction levels of detail. You can take entire courses on microscopy and still have more to learn...

... you can slowly release the plunger. Remove the entire pipette from the container making sure not to touch...

...the solution heats up.). Caution HOT! Be careful stirring, eruptive boiling can occur. Pro-Tip It is a good...

...proceeding with DNA preparation. Pro-Tip If your entire overnight culture cannot fit into a single centrifuge...

...antibiotic in culture, or remove the antibiotic entirely. If the antibiotic is reduced or removed from ...

...Figure 2 Using the box tool, draw a box around the entire first gel lane (as in Figure 2). Select Analyze...

...which should be on the same focal plane. Scan the entire plate and make note of each well in which you see...

...liquid up and down 10–20 times. Gently cover the entire dilution plate with Microseal adhesive seal - do...

...can dramatically plateau and sometimes fall off entirely if the column or reagents are significantly overloaded...