... (Zhang et al., 2015). In this system a modified TIR-1 protein can degrade degron-tagged proteins in an...Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit from the Jorgensen lab. SapTrap...Project: The Complete Gene Expression Map of an Entire Nervous System." Neuron 99.3 (2018): 430-433. PubMed...

...coli azi-donorleucine (Anl) Bacterial ATG David Tirrell 62598 pKPY514 phenylalanyl-tRNA synthetase subunit...p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 62599 pKPY197 phenylalanyl-tRNA synthetase (CePheRS...p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 63177 pMarsL274G methionyl-tRNA synthetase (L274GMmMetRS...musculus azidonorleucine (Anl) Mammalian ATG David Tirrell 64915 pMAH-POLY tyrosyl-tRNA synthetase E. coli...musculus azidonorleucine (Anl) Mammalian ATG David Tirrell 91705 pSupAR-MbPylRS(DiZPK) PylRS M. barkeri photocrosslinkers...

...pOpen-NotIR (GC) NotI (GC-rich recognition site) 165508 pOpen-HindIIIR HindIII 165526 pOpen-PstIR PstI ...

...the body and have the capacity to give rise to an entire organism. iPSC technology removes the possible ...endogenously-tagged gene variants or browse Addgene’s entire collection of Stem Cell Research Plasmids . For...

...Coronavirus Plasmids & Resources page outlines our entire COVID-19 collection. Additionally, we have linked...

...individual clones, or the pooled library containing the entire pooled collection, can be purchased at Addgene....

...to limit the spread of infection throughout the entire population. Type VI enzymes that function in mammalian...

... are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...

...plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description Gene/Insert ...

...to limit the spread of infection throughout the entire population. ID Plasmid Promoter PI Publication ...

... Propionate-inducible expression vector (see entire pPro collection ) Arrowsmith Lab...

...generate large-scale genetic changes, including moving entire genes to different locations, by combining other...you’ll need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then ...

...of gRNAs - A given CRISPR library may target the entire genome or a specific class of genes (subpools)....

...Internal Reflection Microscopy (TIRF) Total Internal Reflection Microscopy (TIRF) is a technique used to only...Internal Reflection Fluorescence (TIRF) microscope can be used. TIRF only excites fluorophores within ...the microscope objective evenly illuminating the entire (wide) field of view. Fluorescent light traveling...microscopy project excitation light through the entire axial volume of a sample. Therefore, when a large...limiting photobleaching. Similar to widefield, the entire field of view is excited at one time and captured...light is projected from the objective so that the entire field of view is bathed with an even illumination...emission light from fluorophores higher in the sample. TIRF is also very gentle on the sample as the majority...

... (XFP) of different colors to label an organ or entire organism. Because each cell can have multiple copies...expressed. Each cell remains the same color for its entire lifetime and daughter cells retain the same color...expressed in a single cell maintains its color over the entire length of the cell, even axons of up to 100 microns...downstream of a neural-specific promoter, or the entire fish could be labeled by injecting Brainbow at ... lines that allow for multicolor labeling of an entire organism or a specific tissue. XFPs are introduced...

...they used CRISPR to create OsTIR1-expressing cell lines by inserting OsTIR1 into the "safe harbor" AAVS1...pooled libraries enable researchers to screen the entire genome for genes that regulate a wide variety of...the NCC-1 library of approved drugs against the entire kit; a parallel analysis that successfully identified...inhibitors are often non-selective, affecting the entire genome and not specific loci. To overcome this ...expressed in a cell that also expressed auxin-sensitive TIR1, any protein fused with their AID tag could be targeted...AAVS1 locus (a tet-inducible OsTIR1 plasmid is also available). Second, they co-transfected short-arm donor...

...induces degradation. In plants, auxin binds to TIR1 of the SCF-TIR1 ubiquitin ligase complex, promoting its ... mAID and expressing the essential plant protein TIR1 in non-plant cells, which interacts with the cells..., the Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor, sufficiently suppresses basal degradation...

.... In this event, the degron domain shuttles the entire tagged protein to the proteasome for degradation...must be AID-tagged with the 25 kDa tag. Next, the TIR1 F-box protein must be expressed (inducibly, ectopically...ectopically, stably… it’s your call). The TIR1 F-box protein will associate with the endogenously expressed...of auxin hormone indole-3-acetic acid (IAA), the TIR1 F-box associated E3 ligase complex will ubiquitinate...

...focus on our development instead of building the entire ecosystem Proven performance with other bioinformatics..., so our QC team could initiate and monitor the entire pipeline. Benefits of Nextflow for bioinformatics...pieces of software that depend on each other for the entire package to run. The small, portable system provided...process was added, we progressively constructed the entire workflow in the Nextflow script. With our completed...

...the success of the company is attributed to the entire Addgene family; the depositors and requesting scientists...headed to the Museum of Science in Boston, where the entire Blue Wing was set aside for the event. As guests...family Perhaps the most heartfelt portion of the entire night was when Melina and Ken called their parents...