... (Zhang et al., 2015). In this system a modified TIR-1 protein can degrade degron-tagged proteins in an...Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit from the Jorgensen lab. SapTrap...Project: The Complete Gene Expression Map of an Entire Nervous System." Neuron 99.3 (2018): 430-433. PubMed...

...Internal Reflection Microscopy (TIRF) Total Internal Reflection Microscopy (TIRF) is a technique used to only...Internal Reflection Fluorescence (TIRF) microscope can be used. TIRF only excites fluorophores within ...the microscope objective evenly illuminating the entire (wide) field of view. Fluorescent light traveling...microscopy project excitation light through the entire axial volume of a sample. Therefore, when a large...limiting photobleaching. Similar to widefield, the entire field of view is excited at one time and captured...light is projected from the objective so that the entire field of view is bathed with an even illumination...emission light from fluorophores higher in the sample. TIRF is also very gentle on the sample as the majority...

... (XFP) of different colors to label an organ or entire organism. Because each cell can have multiple copies...expressed. Each cell remains the same color for its entire lifetime and daughter cells retain the same color...expressed in a single cell maintains its color over the entire length of the cell, even axons of up to 100 microns...downstream of a neural-specific promoter, or the entire fish could be labeled by injecting Brainbow at ... lines that allow for multicolor labeling of an entire organism or a specific tissue. XFPs are introduced...

...they used CRISPR to create OsTIR1-expressing cell lines by inserting OsTIR1 into the "safe harbor" AAVS1...pooled libraries enable researchers to screen the entire genome for genes that regulate a wide variety of...the NCC-1 library of approved drugs against the entire kit; a parallel analysis that successfully identified...inhibitors are often non-selective, affecting the entire genome and not specific loci. To overcome this ...expressed in a cell that also expressed auxin-sensitive TIR1, any protein fused with their AID tag could be targeted...AAVS1 locus (a tet-inducible OsTIR1 plasmid is also available). Second, they co-transfected short-arm donor...

...induces degradation. In plants, auxin binds to TIR1 of the SCF-TIR1 ubiquitin ligase complex, promoting its ... mAID and expressing the essential plant protein TIR1 in non-plant cells, which interacts with the cells..., the Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor, sufficiently suppresses basal degradation...

.... In this event, the degron domain shuttles the entire tagged protein to the proteasome for degradation...must be AID-tagged with the 25 kDa tag. Next, the TIR1 F-box protein must be expressed (inducibly, ectopically...ectopically, stably… it’s your call). The TIR1 F-box protein will associate with the endogenously expressed...of auxin hormone indole-3-acetic acid (IAA), the TIR1 F-box associated E3 ligase complex will ubiquitinate...

...focus on our development instead of building the entire ecosystem Proven performance with other bioinformatics..., so our QC team could initiate and monitor the entire pipeline. Benefits of Nextflow for bioinformatics...pieces of software that depend on each other for the entire package to run. The small, portable system provided...process was added, we progressively constructed the entire workflow in the Nextflow script. With our completed...

...the success of the company is attributed to the entire Addgene family; the depositors and requesting scientists...headed to the Museum of Science in Boston, where the entire Blue Wing was set aside for the event. As guests...family Perhaps the most heartfelt portion of the entire night was when Melina and Ken called their parents...

...Testing AAV inventory for the entire collection! We are expanding our entire control AAV inventory to give...in the last few months. You can also browse our entire AAV inventory. Our new AAVs include: EGFP-expressing...

...appropriate plasmids, and code the entire page. That would be entirely too much work for one person, it ... expert) the end result is high quality and the entire process is completed more efficiently. Listen to...

...Passion? A love of learning? No. Or at least not entirely. What truly unites us all is the frustration of... to the day before, which got rid of the issue entirely. In many cases, the answer isn't to work harder...ability to focus, especially on days when I was tired, stressed, or rushing. I would spend the time quietly...

...primarily negative. Stupid, overwhelmed, discouraged, tired…words that indicated there were many barriers between...paper.  Of course, when you think about writing an entire scientific paper, you may feel it’s overwhelming... writing. You can use them to help you write an entire manuscript, or you could use them to write alt ...

...selective plane illumination, TIRF or spinning disk confocal use entirely different excitation power regimes...Plasmids for Subcellular Localization Browse the entire Fluorescent Protein Collection ...

...system relies on a transport inhibitor response 1 (TIR1) expressed under a desired promoter with a specific...) sequence attached to your protein of interest. TIR1 interacts with endogenous Skp1 and Cul3 proteins...ubiquitin ligase complex. In the presence of auxin, this TIR1 complex binds to the degron sequence ultimately ...

...Most organisms have less than five enzymes in the entire proteome that are biotinylated. In E.coli for example...responsible for biotinylating only one lysine in the entire organism on the biotin carboxyl carrier protein...recall, BirA biotinylates only one lysine in the entire E.coli proteome. Using this knowledge, scientists...

...which is ideal for observing and manipulating the entire developmental cycle. A majority of studies involving...most significant advances is the sequencing of the entire axolotl genome, revealing an abundance of genes...year 2000, becoming the first plant to have its entire genome sequenced (Koornneff and Meinke, 2010). ...

...idea, but they might feel disconnected from the entire scientific process.  I've spent a lot of time debating...debating how to fix this.  As scientists, we work tirelessly in the lab where the groundbreaking research ...training as an academic scientist. I’ve worked tirelessly to get the publications, grant funding, and teaching...

...me to join the committee because I had recently retired from Harvard, and it let me keep my toe in the ...little bit?”    What are you doing to enjoy your retirement? I’m also on the advisory board for Art in Giving...

...only makes CRISPR screen results available to the entire biomedical research community, but also allows ...identifier, which can also be used to search the entire CRISPR database for particular screens of interest...

...long, skinny things") and generally exuding a very-tired-but-very-excited attitude. Riding up to the resort...Montana! Though sadly I will not be attending the entire meeting, Monday's talks alone were worth the trip...