...includes all of the precautions needed in BSL-1, however there are additional precautions that lie beyond ...infection or disease in healthy humans. Many of the precautions are meant to protect individuals from potential..., but not so tight that you cannot perform your duties. Be sure to try out different sizes to know your... nitrile gloves as an alternative. For extra precautions, tuck your sleeves into your gloves to prevent...

... you’ll need to make buffers, media, or other solutions. A key part of this task is making sure you’re... weighing all reagents precisely to create the solution you expect. Understanding how to obtain the correct...important to ensure the accurate composition of your solutions and buffers. Now that you have your reagents weighed... weighed out, you can begin making your solutions!...

...flame. Spray down the bench with a 70% ethanol solution and wipe down with a paper towel. Count out the...molten gel mix, you should create a 1000x stock solution. For example: If you’ll be preparing plates with... 100 ug/mL ampicillin, you should make a stock solution of 100,000 ug/mL (100 mg/mL). Simply measure out...technique. Swirl the agar bottle to ensure even distribution of the antibiotic throughout the agar. Open ...

...sure to ‘blank’ the spectrophotometer using the solution the DNA is resuspended in, but with no DNA added...despite the accuracy of the NanoDrop, if two consecutive samples have significantly different concentrations...and consistent readings from DNA in a buffered solution. Reference Page Top Index...

...gently mix. Notes: Make the 50% glycerol solution by diluting 100% glycerol in dH20. Snap top tubes are...5-6 times). Make sure that you see one uniform solution, and there are no layers present. Be sure to label...

...do you get better resolution of bands? A couple simple ways to increase the resolution (crispness) of your...agarose gel, including tips to help improve the resolution and separation of bands. Protocols...

...includes all of the precautions needed in BSL-1, along with additional precautions to prevent injuries...use 70% ethanol, quaternary ammonium, or bleach solutions. Ask your lab supervisor what is available and...

...image and the resolution your microscope can achieve. You achieve the highest resolution when your image...magnification (the ability to make an image larger) and resolution (the ability to distinguish between two discrete...

...the bottom before placing it in the PCR machine. Diluting Primers Most people order primers from a company...concentration of each primer (10uM) by making a 1:10 dilution of the stock. For example, add 100µl of primer...cations such as Mg 2+ and Mn 2+ stabilize the buffer solution. These cations can also be used for PCR-mediated...

...with water. You may use a 10% bleach solution or a 70% ethanol solution to wipe down the inside of the water...

...around the center (see image to right). Even distribution ensures that the centrifuge is balanced. Using...given as Relative Centrifugal Force (RCF) or Revolutions Per Minute (RPM), these are not the same units...

...polyacrylamide gel electrophoresis alongside serial dilutions of a standard antibody of known concentration....labs use unpurified tissue culture supernatant routinely without any issues. Plot the mg/mL versus the ...

...1 Reagent A to Reagent B dilution of the BCA assay. Prepare serial dilutions of the BSA standard that ...

...standard labeling system for all your objects/solutions. Sterilize your lab bench by spraying it down ...

...concentration (100 mg/mL, 1000x) carbenicillin stock solution in sterile water (or other antibiotic) E. coli...

...Isolating a Monoclonal Cell Population by Limiting Dilution Generate monoclonal cell lines from a polyclonal...

...more colonies if you use 1 μl of a 1:5 or 1:10 dilution rather than 1 μl directly....