... Distribution to Industry Featured Collections Search Plasmids...agreement between the recipient and the depositing institution(s). It governs the transfer of biological materials...distribute plasmids on behalf of the depositing institutions. These agreements indicate the language which...which we are allowed to use for distribution. Please see the Sample Agreements on our Technology Transfer... by an authorized signatory at the recipient institution. An authorized signatory is someone who can execute...execute legal documents on behalf of the recipient institution. Once the MTA has been signed, it can be submitted...order will be processed and shipped. Industry Distribution Policy A portion of Addgene’s collection is ...

...equivalents and see what we can learn from their evolution. Southern blotting and quantitative real-time... less expensive sequencing fuels a sequencing revolution that still prevails. Sequencing whole genomes... evolution...

...examples that have guided your resolution processes in the past. Types of Resolution There are of course many...can do it. If Party X proposes solution A, and Party Y proposes solution B, with proper guidance X and ...to create solution C. And if a conflict resolver does their job well, not only will solution C be better...realized I might have some minor skills in conflict resolution after college, when I discovered that many of...any of their disagreements. In fact, conflict resolution isn’t just about resolving conflicts between ...meant to be a comprehensive guide to Conflict Resolution. There are countless classes and resources that...Conflict Before explaining my approach to conflict resolution, it’s important to recognize that there are different...

...insertion site.   The Sleeping Beauty transposon system The Sleeping Beauty (SB) transposon system is one...gene-of-interest, either constitutively or regulated by doxycycline. The Sleeping Beauty transposase is proving... Mates L, et al. Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust...can translocate within the genome. The Sleeping Beauty transposase gains its name from the fact that it...changes. The basic functional version of the Sleeping Beauty transposon (SB10), developed in 1997, continues...efficiencies of integrating viral vectors. New Sleeping Beauty transposase vectors Even more recently, Eric Kowarz...transposase system - one set of vectors for robust constitutive expression (pSBbi derivatives) and one set of...

...and highlights some of the latest breakthrough therapeutic approaches. What is Rett Syndrome? Caused by ...dysfunction, distinctive hand movements, and show autistic-like behaviors.  The role of MECP2 in Rett Syndrome...physiological levels. Much research is now focused on therapeutic strategies that use gene replacement therapy,...combined technical expertise to develop a viable therapeutic, the researchers developed adeno-associated viral... repair the mutation (Le et al., 2019). Beam Therapeutics, co-founded by Harvard Investigators and Addgene... Rett syndrome program being pursued at Vico Therapeutics, co-founded by Gail Mandel. There are numerous...

...with some solutions based on what I’ve learned. I hope you can apply some of these solutions to your own...Reproducibility. The minisymposium focused on solutions to reproducibility issues in the biological sciences...of reproducibility issues and their potential solutions. Prior to this event, I gave my own talk on reproducibility...understanding how the body works, generating new therapeutics, and sometimes formulating new biological concepts...ultimately wasting both time and money. The obvious solution to this problem would be for traditional journals...issues are complicated, we have many potential solutions that could save both your lab and the entire research...

...quickly following the tried and true process of evolution. Watch this video showing bacteria evolve antibiotic...resistance genes survive and multiply. Factors contributing to the development of resistance include (Figure...before this information becomes available. For routine infections, like strep throat, testing for antibiotic... however, is fight smarter, not harder. We can utilize tactics like rotating antibiotic treatments, so...

...field, let's look at how prokaryotes originally utilized CRISPR/Cas as a powerful and adaptive defense ...prokaryotes developed an adaptive immune system that utilized various cas genes not only to store a record of...proteins and PAM sequences. A total of eight evolutionarily conserved CRISPR subtypes comprised of more..., 83% of archaeal and 45% of bacterial genomes utilize this system (4). Makarova et al. grouped the systems...subtypes using polythetic classification based on evolutionary similarity (5). Type II CRISPR/Cas is the system...against invading phages. Rising to the challenge - Evolution of anti-CRISPR genes in phages The CRISPR/Cas ...

...developed today. In this post, I will highlight the evolution of the  Brainbow multicolor labeling system - ...- a perfect example of the continued utility of Cre-lox. Check out our previous blog post, Plasmids 101...highlights the trend in biology towards single-cell resolution imaging. Sanes and Lichtman endeavored to create... of stained sections, were limited due to low resolution or were very time-intensive, necessitating the...

... have taken researchers to the threshold of a revolution in modern neuroscience, an era in which technologies...26135556. Hudry, Eloise, and Luk H. Vandenberghe. "Therapeutic AAV gene transfer to the nervous system: a clinical...

....   Sleeping Beauty, a transposon system from ancient fish Surprisingly, Sleeping Beauty is not only a... “awakened from a long evolutionary sleep,” it was aptly named Sleeping Beauty (Ivics et al., 1997). We...rooms such as Groucho, Sonic Hedgehog, Sleeping Beauty, Cookie Monster, Spaghetti Squash, Tinman, and ...but also a biological transposon system. Sleeping Beauty is a synthetic transposon system that can be used... transposon system? Scientists can use Sleeping Beauty transposons as a non-viral system to integrate ...

... a 1:100 dilution of the homogenized cell solution by adding 10 µL homogenized cell solution to 990 µL...Cloning by Limiting Dilution Isolating a Monoclonal Cell Population by Limiting Dilution You may also like...cell solution later in this protocol. Isolating a Monoclonal Cell Population by Limiting Dilution Isolate...homogenized cell solution is transferred. Transfer cells from the homogenized cell solution into the conditioned...cell solution at a concentration of 5 cells/mL. Prepare approximately 10 mL of this cell solution for ...medium, prepare this cell solution in fresh complete medium. This 5 cells/mL solution will be used to seed ... quantitate the homogenized cell solution. Homogenized cell solution concentration: 4 × 10 5 cells/mL ...

...iAChSnFR none Constitutive 1 Looger 137955 pAAV.CAG.iAChSnFR CAG iAChSnFR none Constitutive 1, 9 Looger ... none Constitutive 1, 5, rg* Looger 176753 AAV-mDlx-jGCaMP8f-WPRE Dlx jGCaMP8f none Constitutive 1, 9,...jGCaMP8f none Constitutive 1, 9 Looger 179254 AAV-CAG-jGCaMP8f-WPRE CAG jGCaMP8f none Constitutive 1, 9, rg...ribo-jGCaMP8s none Constitutive 1 Fyhn 169256 AAV-hSyn-Soma-jGCaMP8s Syn soma-jGCaMP8s none Constitutive 1, 9, rg...none Constitutive 1, 5, 9, rg* Looger 176755 AAV-mDlx-jGCaMP8s-WPRE Dlx jGCaMP8s none Constitutive 1, 9...jGCaMP8s none Constitutive 1, 9 Looger 179256 AAV-CAG-jGCaMP8s-WPRE CAG jGCaMP8s none Constitutive 1, 9, rg...ribo-jGCaMP8m none Constitutive 1 Fyhn 169257 AAV-hSyn-Soma-jGCaMP8m Syn soma-jGCaMP8m none Constitutive 1, 9, rg...

... gfaABC1D tdTomato Constitutive 5 Khakh 50465 pAAV-hSyn-EGFP hSyn EGFP Constitutive 1, 2, 5, 8, 9, rg*...hSyn tdTomato Constitutive 5 Zeng 58909 pAAV-GFAP104-mCherry GFAP104 mCherry Constitutive 5 Boyden 59462...GFA104 EGFP Constitutive 5 Haydon 104055 pAAV-CAG-eYFP CAG EYFP Constitutive 2, 5, rg*, PHP.eB Gradinaru... NLS-GFP Constitutive PHPeB Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Constitutive 1, 2, 5, ...CMV LacZ Constitutive 5, 8 Wilson 105532 pAAV.CMV.ffLuciferase.SV40 CMV ffLuciferase Constitutive 8 Wilson...TBG none Constitutive 8 Wilson 105541 pENN.AAV.CamKII0.4.eGFP.WPRE.rBG CamKII EGFP Constitutive 1, 5 Wilson...pAAV.TBG.PI.eGFP.WPRE.bGH TBG EGFP Constitutive 8 Wilson 105542 pENN.AAV.CB7.CI.eGFP.WPRE.rBG CB7 EGFP Constitutive 1, 2, 5, 8...

...Venus Constitutive 1, 9 Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell-3 Dlx ChR2 mCherry Constitutive 1, ...t) mScarlet Constitutive 9 Harvey 59170 pAAV-Syn-Chronos-GFP Syn Chronos GFP Constitutive 1, 5, rg* Boyden... ChETA EYFP Constitutive 9 Deisseroth 135633 pAAV-S5E2-C1V1-eYFP E2 C1V1 EYFP Constitutive 1, 5, 9, PHPeB...EYFP Constitutive 1, 9 Deisseroth 26972 pAAV-hSyn-eNpHR 3.0-EYFP Syn eNpHR 3.0 EYFP Constitutive 5 Deisseroth...dependent Flp dependent Cre and Flp dependent Constitutive Serotype AAV1 AAV2 AAV5 AAV8 AAV9 AAVrg PHPeB...135634 pAAV-S5E2-ChR2-mCherry nEF ChR2 mCherry Constitutive 1, 9, PHPeB Dimidschstein 137142 pAAV-nEF-Con...pAAV-CaMKIIa-hChR2(H134R)-EYFP CaMKII ChR2/H134R EYFP Constitutive 1, 2, 5, 9 Deisseroth 26973 pAAV-hSyn-hChR2(...

... or mammalian expression (Constitutive or Cre-dependent) Directed Evolution of a Selective and Sensitive...and Archon 2 (Constitutive or Cre-dependent) A robotic multidimensional directed evolution approach applied...Fast Genetically Encoded Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically Encoded...High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance calcium sensors...calcium sensors for imaging neural activity (Constitutive or Cre-dependent) Ultrasensitive fluorescent...mRuby2 for imaging neural activity (GCaMP6f/m/s) (Constitutive or Cre-dependent) Cell-specific restoration ...Enriched expression of GCaMP6s or GCaMP6f in axons (Constitutive or Cre-dependent) In vivo measurement of afferent...

...by PCR, predictability of loss-of-function, and utility for the study of non-coding elements. This approach...markers for selection 1 . Other constructs may be utilized, such as pX458 (Addgene plasmid ID 48138) or pX459...sequencing primer; other flanking primers may be utilized. Choose a sequence-verified colony and inoculate...Resuspend 2 x 10 6 cells in 100 μl of electroporation solution and add to electroporation cuvette. Add 5 μg of...attempting genome editing. Immediately transfer solution from cuvette into 1 ml of culture media after ...time between electroporation and transferring the solution into media to enhance cell viability. Incubate...round-bottom plates using sorter or by using limiting dilution at 30 cells per 96-well round-bottom plate. Optimize...

...for RNAi Constitutive Lentiviral Cre-regulated Lentiviral Tet-regulated Lentiviral Constitutive Retroviral...design and delivery. Constitutive Lentiviral shRNA Lentiviral vectors for constitutive shRNA expression ID... and Didier Trono Constitutive Retroviral shRNA Retroviral vectors for constitutive shRNA expression ID...interference (RNAi) experiments at Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA... vectors for cloning in shRNAs. Plasmids for constitutive expression as well as those that allow for conditional...

...options, such a 4-base pair codons, have also been utilized. A tRNA that recognizes this codon. An aminoacyl-tRNA... can be mutated and screened through directed evolution to charge the tRNA with a different amino acid...and evolve proteins for various purposes from therapeutics to biopolymers. Tips for Success Before beginning... IBBN (G2) synthetase M. jannaschii 4-(2′-bromoisobutyramido)-phenylalanine (IBBN) and structurally analogous...Howard Hang 126035 pEVOL-ABK PylRS M. barkeri 3’-azibutyl-N-carbamoyl-lysine (AbK) Bacterial TAG Andrea ...Expresses yeast TrpRS/tRNA pair instead. For directed evolution of the liberated EcTrpRS/tRNA pair to incorporate...