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  1. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ... as opposed to transiently-transfected cells, increases reproducibility, as it eliminates the variation... vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature Methods...

  2. Pouring LB Agar Plates

    Type
    Protocol
    ...maximum of ~100 colonies Pro-Tip If you will be screening a large number of colonies, we recommend using...your antibiotic to the molten gel mix, you should create a 1000x stock solution. For example: If you’ll ...

  3. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...an antibody. This protocol will describe how to create a standard curve using known concentrations of ...be empirically determined. The dilution series created in section 1, step 2 is a suggested starting point...

  4. AAV Production in HEK293 Cells

    Type
    Protocol
    ...harvesting. The addition of sorbitol has been shown to increase viral titers by up to 1.8-fold ( Rego et al., ...sodium bicarbonate, sorbitol has been found to increase viral titers by up to 1.8-fold. See the recipe...

  5. Protocol - How to Design Primers

    Type
    Protocol
    ...also needs to avoid primer-primer annealing which creates primer dimers and disrupts the amplification process...

  9. Fluorescence Titering Assay

    Type
    Protocol
    ... remove cells and debris. Lentiviral titer can decrease during cycles of freeze-thaw. If you are freezing...

  15. Western Blot

    Type
    Protocol
    ... have moved out of the wells and into the gel. Increase the voltage to 150 V and continue running the ...
Showing: 21 - 35 of 35 results