Skip to main content
Addgene
Showing: 1 - 6 of 6 results
  1. CRISPR Guide

    Type
    Guide
    ...have been used for dynamic tracking of repetitive and non-repetitive genomic loci, chromosome painting, and...labeled proteins to the site. Chromosome-specific repetitive loci can be efficiently visualized in living ...multiple target sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering...924–927. PMID: 30377362 Casini, A., Olivieri, M., Petris, G., Montagna, C., Reginato, G., Maule, G., Lorenzin... N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J. J., Aryee, M. J., & Joung, J. .... Cell . 184 (9), 2503-2519.e17. PMID: 33838111 Peters, J. M., Koo, B., Patino, R., Heussler, G. E., Hearne... S., Silvis, M. R., Harden, M. M., Osadnik, H., Peters, J. E., Engel, J. N., Dutton, R. J., Grossman, ...
  2. Lentiviral Guide

    Type
    Guide
    ...reasons, transfer plasmids are all replication incompetent and may contain an additional deletion in the...usually encodes for VSV-G Safety Safe. Replication incompetent: Uses 3 separate plasmids encoding various HIV...HIV genes. Safer. Replication incompetent and always SIN: Uses 4 plasmids instead of 3 and eliminates ...: The potential for generation of replication-competent lentivirus The potential for oncogenesis The potential... potential for generation of replication-competent lentivirus is addressed by the design of the vectors...Import Is Mediated by a Central DNA Flap. Zennou V, Petit C, Guetard D, Nerhbass U, Montagnier L, Charneau...
  3. Adenovirus Guide

    Type
    Guide
    ...the viral vector renders the virus replication incompetent. E3 is involved in evading host immunity and ...either 293 or 911). To avoid creating replication competent virus, you should not serially propagate your ... of recombination events creating replication-competent virus increases with each round of amplification...amplification. What is RCA? RCA stands for r eplication c ompetent a denovirus. This may occur when a crossover event...; Its deletion renders the virus replication incompetent E3 Non-essential gene involved in immune evasion...
  4. Retrovirus Guide

    Type
    Guide
    ...lentivirus guide. Are γ-retroviruses replication incompetent? Yes, as with lentiviruses, the genes required...
  5. Cloning

    Type
    Guide
    ... has difficulty assembling DNA fragments with repetitive sequences and DNA that ends in sequences that...
Showing: 1 - 6 of 6 results