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  1. Participate in the Pets of Addgene Scavenger Hunt

    Type
    Blog Post
    Published
    April 1, 2021, 1:15 p.m.
    ...pictures of Addgene pets with Addgene-branded clothing on the blog. Below each photo, the pet gives you a clue...some of us, that means spending more time with our pets. These new “co-workers” have made their way onto...the last year. Now, we’ve collected photos of our pets with Addgene gear, and have unleashed them all over...

  2. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...lab. How to make your own competent cells It’s easy to make your own competent cells. If you need a high...from purchasing competent cells. However, for most everyday cloning, homemade competent cells will work...OpenWetWare Preparing chemically competent cells (protocol)  Preparing electrocompetent cells (protocol)  Untergasser...Preparation of chemically competent cells (protocol) Preparation of electrocompetent cells (protocol) At...produces 20mL of competent cells, enough for 800 transformations when using 25uL competent cells per reaction...Transformation of chemically competent cells (protocol)  Transformation of electrocompetent cells (protocol)  ... time efficient. We do this by making our own competent cells and using a little-known reagent for streamlining...

  3. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...terminal) 47327 pET-28b-Cas9-His 6xHis (C terminal); NLS (C terminal) 62933 pET-Cas9-NLS-6xHis ...6xHis (C terminal); NLS (C terminal) 62934 pET-NLS-Cas9-6xHis 6xHis (C terminal); NLS (N terminal...

  4. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ... a virtual soup lunch, and sharing photos of our pets. #SocialDistancing may send a wrong message for...or ordering take-out) or volunteering to foster a pet, for example. If you are looking for some resources...

  5. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...captured over the years, including an album of Addgenie pet photos. The famous Addgene jacket, home to a questionable...

  6. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...large amounts of protein in bacterial cells: the pET, pRSET, Gateway pDEST, and pBAD vectors for example...protein is toxic to E. coli. Of all the vectors, pET, under the control of the T7 lac promoter and induced...

  7. #DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La

    Type
    Blog Post
    Published
    Dec. 2, 2021, 2:15 p.m.
    ...DeckTheLab themed nativity scene, courtesy of our house pets.. 😁 pic.twitter.com/qDylF8TdHH — Matthijs Dorst...traditional approach. You might decorate your lab in pipette tip snowflakes, hang stockings filled with tube...

  8. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...DeckTheLab themed nativity scene, courtesy of our house pets.. 😁 pic.twitter.com/qDylF8TdHH — Matthijs Dorst.... Nonetheless, Addgene’s annual Deck the Lab competition drew upon the creativity of the community, who...place-winning entry doubled as their lab’s decoration competition, and each lab member used an impressive variety... started last year: The Christmas Decoration Competition! #ScienceFun #ZoomParty @Addgene pic.twitter....edition, the MicrobiGals created glow in the dark petri dish ornaments and microbe cookies! 🤗 🤗 🤗 We...

  11. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ... she enjoys reading, petting dogs, and winning the office Just Dance competition.    Special thanks to...production on a protocol video. Don't worry - no pipettes were harmed in the making of this photo!  Here...

  12. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...derivative) A Relaxed pBR322 ~15-20 pMB1 A Relaxed pET ~15-20 pBR322 A Relaxed pGEX ~15-20 pBR322 A Relaxed...the same ori are incompatible because they will compete for the same machinery, creating an unstable and...

  16. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...tumble out of the sink and on to a poor unsuspecting pet.  The software equivalent to this would be staying...

  18. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...operator. Can be induced by IPTG. Commonly found in pET vectors. Very tightly regulated by the lac operators... repression in the presence of glucose or by competitive binding of the anti-inducer fucose Weaker. Commonly...

  19. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...Norway rat, from an obnoxious pest to a laboratory pet. ELife, 9. https://doi.org/10.7554/elife.50651 Vieira...

  20. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...agent (0.4 mM IPTG is a good start if you’re using a pET vector) and antibiotic for plasmid selection. Remove... for protein overexpression in E. coli, such as pET30a. If working with DNA that is refractory to enzymatic...your resultant plasmid libraries into chemically competent E. coli and analyze transformants for appropriately...order to reduce the chances of some clones being outcompeted by others in liquid culture, dilute and plate...transformation should be performed using a highly competent strain that you can use for long-term library ...probing for cell-binding activity.  First, cut out 20 Petri dish-sized circles of nitrocellulose membrane and...inhibitor cocktail. Line the bottom of 20 empty Petri dishes with thick filter paper and saturate each...
Showing: 1 - 20 of 218 results