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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 3 results for: donor

Showing: 1 - 3 of 3 results

  1. Cloning

    Type
    Guide
    ...This fragment can then be cloned into a gateway donor plasmid which contains compatible attP sites via...Now that your DNA fragment has been cloned into a donor plasmid, it can be rapidly shuttled into any compatible...can clone your gene of interest one time into a donor plasmid (or acquire one that already has your gene...allowing for rapid cloning of PCR products into donor plasmids without the need for restriction enzyme...

  2. CRISPR Guide

    Type
    Guide
    ...specific genomic edits by copying from a DNA donor template . The donor template contains the desired sequence...out integration of donor DNA. Both types of CASTs have been found to achieve donor DNA integration for...cell along with the gRNA(s) and Cas enzyme. The donor DNA contains the desired edit as well as additional...homology arms. Depending on the application, the donor DNA may be a single-stranded oligonucleotide, double-stranded...for large edits or insertions. When designing the donor template, incorporating mutations that prevent further...should alter the PAM site or target sequence in your donor DNA with a silent mutation that does not change ...recombinase or phage derived serine integrases, insert donor DNA at “landing sites” defined by relatively short...

  3. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...mechanisms. One of these methods uses splice site donor and acceptor sequences to reconstitute the two pieces...contains the 5' portion of the gene and a splice donor sequence at its 3' end, while the second vector ...
Showing: 1 - 3 of 3 results