...This fragment can then be cloned into a gateway donor plasmid which contains compatible attP sites via...Now that your DNA fragment has been cloned into a donor plasmid, it can be rapidly shuttled into any compatible...can clone your gene of interest one time into a donor plasmid (or acquire one that already has your gene...allowing for rapid cloning of PCR products into donor plasmids without the need for restriction enzyme...

...AAV transfer plasmids, the first with a 3’ splice donor and the second with a 5’ splice acceptor. When these...