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  1. Church Lab CRISPR Plasmids

    Type
    Collection
    ... 48661 SK-YFP-SPNM-B Bacterial SP and NM repression YFP reporter: protospacer B 48662 SK-YFP-ST1-B Bacterial...ST1 repression YFP reporter: protospacer B 48663 SK-YFP-TD-B Bacterial TD repression YFP reporter: protospacer...protospacer B 48664 SK-YFP-NM-A Bacterial NM repression YFP reporter: protospacer A 48665 SK-YFP-ST1-A Bacterial...ST1 repression YFP reporter: protospacer A 48666 SK-YFP-TD-A Bacterial TD repression YFP reporter: protospacer...

  2. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pRS416Gal TDP43 WT YFP TARDBP YFP GAL1 ALS Aaron Gitler 27448 pRS416 Gal G294A YFP TARDBP YFP GAL1 ALS Aaron...pRS416 Gal M337V YFP TARDBP YFP GAL1 ALS Aaron Gitler 27450 pRS416 Gal Q331K YFP TARDBP YFP GAL1 ALS Aaron...pRS303 Gal TDP-43 YFP TARDBP YFP GAL ALS Aaron Gitler 27470 pDONR TDP43 WT YFP TARDBP YFP ALS Aaron Gitler...Gitler 29592 426Gal-FUS-YFP FUS YFP GAL1 ALS Aaron Gitler 29593 416Gal-FUS-YFP FUS YFP GAL1 ALS Aaron Gitler...29594 416GPD-FUS-YFP FUS YFP GPD ALS Aaron Gitler 29595 416Gal-SV40NLS-FUS-YFP FUS YFP, SV40 NLS GAL1 ALS...426Gal-FUS-1-170aa-YFP FUS YFP GAL1 ALS Aaron Gitler 29597 426Gal-FUS-1-270aa-YFP FUS YFP GAL1 ALS Aaron ...426Gal-FUS-1-373aa-YFP FUS YFP GAL1 ALS Aaron Gitler 29599 426Gal-FUS-1-413aa-YFP FUS YFP GAL1 ALS Aaron ...

  3. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... NLS mCherry Connie Cepko 37341 pQC NLS YFP IX Nucleus NLS YFP Connie Cepko 158000 pCaggs-NLS-PAmCherry1...miRFP703 Vladislav Verkhusha 1816 Lamp1-YFP Lysosomes Lamp1 YFP Walther Mothes 1817 Lamp1-RFP Lysosomes...Bruchez 45944 pTDpelB-C_sfYFPTwinStrep Periplasmic space PelB signal sequence YFP Thorben Dammeyer 54520... 21741 Clathrin-LCa-EYFP Clathrin-coated vesicles Clathrin, light polypeptide EYFP Xiaowei Zhuang 14437...

  4. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...tdTomato from the CMV promoter pAdx-CMV-YFP 73348 Express YFP with an NLS from the CMV promoter pAdx-CMV-dsRed...

  5. Bacterial Expression Systems

    Type
    Collection
    ...to the periplasm. Series contains vectors with a YFP variant that functions in the periplasm and can be... FLIParaF.Ec-250n 18855 N/A FRET between CFP and YFP Wolf Frommer Reporter for arabinose levels in bacteria...arabinose enhances the FRET signal from the CFP and YFP fluorophores linked by an arabinose binding protein...

  6. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Maturation Structure Plasmids EYFP 513 527 51 6.9 Prone to dimerization pcDNA3-YFP - Mammalian Expression Topaz...Golenbock Lab Plasmids - Includes tagging with CFP, YFP, and mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently...moxVenus - Mammalian Expression SYFP2 515 527 69 6 Monomer (A206K) pSYFP2-C1 - Mammalian Expression mGold...Vectors - Gateway cloning; includes tagging with ECFP, EYFP, DsRed, Cerulean Bacteria Gradia Lab Bacterial Vectors...

  7. CRISPR Plasmids - Tagging

    Type
    Collection
    ...templates for amplification of the tag (eg GFP, Flag, YFP, etc) and selection markers. Two independent selection...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... 2013 Nov 13;7:202. Colin Akerman Chloride (Cl-) YFP-derived sensor for measuring Cl- concentration in...in physiological range A genetically-encoded YFP sensor with enhanced chloride sensitivity, photostability...Looger NADH/NAD+ Fluorescent biosensor of NAD/NADH (RexYFP) Genetically encoded fluorescent indicator for ...

  10. Optogenetics AAV Preps

    Type
    Collection
    ...ET/TC)-EYFP nEF ChR2(E123T/T159C) EYFP Flp dependent 8 Deisseroth 26968 pAAV-Ef1a-DIO ChETA-EYFP EF1a ChETA...E123T/H134R)-eYFP.WPRE.hGH CaMKII ChETA EYFP Constitutive 9 Deisseroth 135633 pAAV-S5E2-C1V1-eYFP E2 C1V1 ...Arch3.3-EYFP nEF Arch3.3 EYFP Cre dependent 8 Deisseroth 137150 pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP nEF Arch3.3...Arch3.3 EYFP Flp dependent 8 Deisseroth 20949 pAAV-double floxed-eNpHR-EYFP-WPRE-pA EF1a eNpHR EYFP Cre dependent...pAAV-CaMKIIa-eNpHR 3.0-EYFP CaMKII eNpHR 3.0 EYFP Constitutive 1, 9 Deisseroth 26972 pAAV-hSyn-eNpHR 3.0-EYFP Syn eNpHR...eNpHR 3.0 EYFP Constitutive 5 Deisseroth 137151 pAAV-nEF-NpHR3.3-EYFP nEF NpHR 3.3 EYFP Constitutive 8 ...NpHR3.3-EYFP nEF NpHR 3.3 EYFP Cre dependent 8 Deisseroth 137154 pAAV-nEF-Coff/Fon-NpHR3.3-EYFP nEF NpHR...

  11. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...eNpHR 3.0-EYFP Karl Deisseroth AV-1-26967M 100049-AAV1 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Karl Deisseroth...pAAV-Ef1a-DIO ChETA-EYFP Karl Deisseroth AV-1-26969P 26969-AAV1 pAAV-CaMKIIa-hChR2(H134R)-EYFP Karl Deisseroth...pAAV-CaMKIIa-eNpHR 3.0-EYFP Karl Deisseroth AV-1-26973P 26973-AAV1 pAAV-hSyn-hChR2(H134R)-EYFP Karl Deisseroth...pAAV-Ef1a-DIO eNpHR 3.0-EYFP Karl Deisseroth AV-5-26968P 26968-AAV5 pAAV-Ef1a-DIO ChETA-EYFP Karl Deisseroth ...pAAV-CaMKIIa-hChR2(H134R)-EYFP Karl Deisseroth AV-5-26972P 26972-AAV5 pAAV-hSyn-eNpHR 3.0-EYFP Karl Deisseroth ...floxed-eNpHR-EYFP-WPRE-pA Karl Deisseroth AV-9-26966P 26966-AAV9 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Karl Deisseroth...E123T/H134R)-eYFP.WPRE.hGH Karl Deisseroth AV-9-26968P 26968-AAV9 pAAV-Ef1a-DIO ChETA-EYFP Karl Deisseroth...

  12. Deisseroth INTRSECT Collection

    Type
    Collection
    ...-fDIO EYFP Flp 55640 pAAV-Ef1a-dDIO hChR2(H134R)-EYFP Dre 55639 pAAV-Ef1a-fDIO hChR2(H134R)-EYFP Flp 126080...proof-of-concept targeting approach in 2014 1 (using EYFP and ChR2-EYFP as payloads). This approach has been broadly...mouse and rat lines. Plasmids In addition to EYFP and ChR2-EYFP, a large number of additional, validated ...pAAV-Ef1a-sCreDIO hChR2(H134R)-eYFP Scre 126081 pAAV-Ef1a-vCreDIO hChR2(H134R)-eYFP Vcre Dual recombinase-dependent...Mutations 55650 pAAV-hSyn Con/Fon EYFP Cre AND Flp 55651 pAAV-hSyn Con/Foff EYFP Cre AND NOT Flp F3/F5 137162...pAAV-Ef1a-Con/Foff 2.0-EYFP Cre AND NOT Flp FRT/F5 55652 pAAV-hSyn Coff/Fon EYFP Flp AND NOT Cre 137129... Con/Fon hChR2(H134R)-EYFP Cre AND Flp 55644 pAAV-nEF Con/Fon hChR2(H134R)-EYFP Cre AND Flp 55647 pAAV-nEF...

  13. Brain Initiative Collection

    Type
    Collection
    ...-CAG-DIO-EYFP An AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the...AAV2 pAAV-CAG-eYFP An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG ...AAV5 pAAV-CAG-eYFP An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG ...AAVrg pAAV-CAG-eYFP An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG ...PHPeB pAAV-CAG-eYFP An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG ...Boyden 117382-AAV2 hSyn1-eYFP An AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoter...Gradinaru 117382-AAV5 hSyn1-eYFP An AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoter...

  14. Brain Armamentarium

    Type
    Collection
    ...minBG-SYFP2-WPRE3-BGHpA DLX2.0 (3xCore version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression...PHPeB CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful...pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3038) Expression of SYFP2 (yellow fluorescent protein...AiP12408 - pAAV-AiE0600m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2408) Expression of SYFP2 (yellow fluorescent protein...pAAV-AiE0779m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044) Optimized expression of SYFP2 (yellow fluorescent...pAAV-AiE0888m_C4-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3863) Enhancer AAV for expression of SYFP2 in midbrain dopamine...pAAV-AiE0441h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3905) Enhancer AAV for expression of SYFP2 in all striatal...

  15. Caltech Systemic Capsids

    Type
    Collection
    ...rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA minBetaGlobin SYFP2 Control Ting 163509 CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3...Dlx mRuby2 Control Gradinaru 104055 pAAV-CAG-eYFP CAG EYFP Control Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP...EF1a mCherry Control Deisseroth 117382 hSyn1-eYFP Syn EYFP Control Gradinaru 135630 pAAV-S5E2-dTom-nlsdTom...-BGHpA hSyn1 H2B-SYFP2 Control Ting 191706 AiP13044 - pAAV-AiE0779m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias...CN3044) minBG SYFP2 Control AIBS , Ting 191720 AiP13038 - pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA (...: CN3038) minBG SYFP2 Control AIBS , Ting 191728 AiP12408 - pAAV-AiE0600m-minBG-SYFP2-WPRE3-BGHpA (Alias... CN2408) minBG SYFP2 Control AIBS , Ting 199773 AiP13863 - pAAV-AiE0888m_C4-minBG-SYFP2-WPRE3-BGHpA (Alias...

  16. Control AAV Preps

    Type
    Collection
    ...Foff 2.0-EYFP EF1a EYFP Cre dependent 8 Deisseroth 137164 pAAV-nEF-Con/Fon/Von eYFP nEF EYFP Cre, Flp ... EGFP Constitutive 5 Haydon 104055 pAAV-CAG-eYFP CAG EYFP Constitutive 2, 5, rg*, PHP.eB Gradinaru 104061...Constitutive 5 Khakh 105622 pAAV.CamKII(1.3).eYFP.WPRE.hGH CamKII(1.3) eYFP Constitutive 1 Deisseroth 105921 pAAV-CBh-mKate2... 1, 2, 5, 8, 9, rg* Deisseroth 117382 hSyn1-eYFP hSyn eYFP Constitutive 5, 8, 9, rg*, PHP.eB Gradinaru...Constitutive 9, PHP.eB Feng 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP Cre dependent 1, 2, 5, 9, rg* Deisseroth 28306... dependent 1 Wickersham 104052 pAAV-CAG-DIO-EYFP CAG EYFP Cre dependent PHP.V1 Gradinaru 83895 pAAV-hDlx-Flex-GFP-Fishell..., 8, 9, rg* Deisseroth 55641 pAAV-Ef1a-fDIO EYFP EF1a EYFP Flp dependent 1, 2, 5, 8, 9, rg* Deisseroth...

  17. Retrograde AAV viral preps

    Type
    Collection
    ...pAAV-Ef1a-DIO EYFP EF1a EYFP, Cre-dependent Control Deisseroth 55641 pAAV-Ef1a-fDIO EYFP EF1a EYFP, Flp-dependent...Fon/Von eYFP nEF EYFP, Cre, Flp and VCre-dependent Control Deisseroth 117382 hSyn1-eYFP Syn EYFP Control...Cre-dependent Control Roth 55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and Flp-dependent Control Deisseroth 28306...Dlx dTomato Control Fishell 104055 pAAV-CAG-eYFP CAG EYFP Control Gradinaru 112677 pOTTC1032 - pAAV EF1a... 3.0-EYFP EF1a Inhibitor, Cre-dependent Optogenetics Deisseroth 26973 pAAV-hSyn-hChR2(H134R)-EYFP Syn ...Deisseroth 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a Activator, Cre-dependent Optogenetics... Deisseroth 55645 pAAV-hSyn Con/Fon hChR2(H134R)-EYFP Syn Activator, Cre and Flp-dependent Optogenetics...

  18. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...C-terminal His tag SYFP2 Yellow Mammalian Express a gene of interest fused to the C-terminus of SYFP2 Clover Green... EYFP connected by 8 GGSGGS repeats pET28CLY9 Peptide linker standard consisting of ECFP and EYFP connected...pET28CLY1 Peptide linker standard consisting of ECFP and EYFP connected by 1 flexible glycine- and serine-containing...pET28CLY2 Peptide linker standard consisting of ECFP and EYFP connected by 2 GGSGGS repeats pET28CLY3 Peptide ...Peptide linker standard consisting of ECFP and EYFP connected by 3 GGSGGS repeats pET28CLY4 Peptide linker standard... standard consisting of ECFP and EYFP connected by 4 GGSGGS repeats pET28CLY5 Peptide linker standard ...standard consisting of ECFP and EYFP connected by 5 GGSGGS repeats pET28CLY6 Peptide linker standard consisting...

  19. AAV Molecular Tools

    Type
    Collection
    ...TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression of EYFP 1 Gradinaru...

  20. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Llama 135219 HS22 pEYFPN1 anti-Homer1 nanobody HS22 Homer1 Mouse Llama 135220 HC20 pEYFPN1 anti-Homer1 nanobody...Llama 135221 HS38 pEYFPN1 anti-Homer1 nanobody HS38 Homer1 Mouse Llama 135223 HC87 pEYFPN1 anti-Homer1 nanobody...
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