...Brainbow. Brainbow 1.0 used the fluorophores RFP (red), YFP (yellow), and membrane-tethered CFP (cyan). Brainbow...switches from the default GFP (green) to RFP, CFP, or YFP fluorescence. In Brainbow 2.1, GFP is tagged with...localize to a different area of the cell than CFP and YFP. The most recent derivation of Brainbow, Brainbow...incorporate a truncated, non-fluorescent form of YFP as the default state of the transgene such that recombination...recombination is necessary to excise the non-fluorescent YFP and activate fluorescence of the downstream OFP, ...can be detected by immunostaining of the truncated YFP. In Thy1-Brainbow mice, some neurons expressed as...called Zebrabow-GateDest containing an RFP, CFP, and YFP downstream of a ubiquitous promoter. Zebrabow is ...

...favorite protein (YFP). You have two options:  Option #1: Affinity tag purification You tag YFP and use an affinity...binding YFP to the column, you wash several times to remove non-specific proteins, and then elute YFP.  Option...the lights, OptoNBs bind YFP and non-specific proteins flow through. To elute YFP, you turn on the blue ... (OptoNBs) purification You skip adding a tag to YFP and instead use OptoNBs. You fill a column with OptoNB...

...and Petsko labs recently engineered hyperfolder YFP (hfYFP), a FP well-suited for the demands of advanced...Anti-GFP [N86/8R] ready-to-use antibody Hyperfolder YFP CRISPR-Csm: A novel RNA targeting system by: Susanna... PMID: 30667360; PMCID: PMC6377228. Hyperfolder YFP- a chemically stable tool for microscopy by: Susanna...Fig. 2 Campbell et al, Nature Methods   Find YFP plasmids here!   Campbell, B.C., et al. Chemically...microscopy experiment, consider hfYFP and LSSmGFP.     Fig. 4: hfYFP retains folding and fluorescence...resolution, electron, and expansion microscopy. hfYFP is not only brilliantly bright, but it also withstands...conditions better than most FPs. The group also tested hfYFP in expansion microscopy as well as correlative light...

...variants of Yellow Fluorescent Protein (YFP). The team identified a new YFP variant, mGold. mGold was found to... as bright to mVenus, the parent YFP, and is the most photostable YFP to date. As such, mGold is expected...

... contains three fluorescent proteins: RFP (red), YFP (yellow) and M-CFP (membrane tethered cyan). Without...expressed. Cre can mediate one of two deletions to allow YFP or M-CFP expression; these deletions are defined ...construct can express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains two tandem invertible...the promoter. The STOP cassette includes a mutant YFP that does not fluoresce, but can be detected via ...

...Several early FRET biosensors were made with CFP and YFP, cyan and yellow variants of EGFP equally capable...capable of dimerizing as EGFP. As a result, CFP and YFP dimers can form in the absence of environmental changes...other GFP family members (superfolder, Emerald, CFP, YFP, BFP, Cerulean, Turquoise, Venus, and Citrine) can...

... mCerulean/Amber, mCerulean/SYFP2A, mTurquoise/mVenus and others. CFP-YFP is still one of the best and...significant overlap in their spectra. The pair CFP-YFP was the first to be used to study protein-protein...popular pair, CFP has five-fold less brightness than YFP. The stoichiometry of donor:acceptor is difficult...His tag SYFP2 Yellow Mammalian Express a gene of interest fused to the C-terminus of SYFP2 Clover ...

...nm. For example, CFP (ex 430 nm / em 474 nm) and YFP (ex 514 nm / em 527 nm) can be imaged together but...multicolor imaging include ones designed for CFP, YFP, and RFP or the Sedat Quad filter set, designed for...

...specificity of their system, Tang et al. found that YFP and CFP can activate certain T-DDOG constructs similarly...GFP), is activated by GFP and derivatives GFP and YFP, but not red fluorescent proteins, as seen with T-DDOGs...

...ShadowY, a novel dark yellow fluorescent protein (YFP) with an excitation peak of 519 nm. These proteins...microscopy) assays. ShadowG is darker than other dark YFP mutants, it reduces the risk of spectral contamination...

...variants blue FP (BFP), cyan FP (CFP), and yellow FP (YFP) were derived. For a breakdown of GFP, it's variants...CFP) excites the fluorescence of another FP (e.g. YFP). FRET only occurs when the distance between the ...

...antibodies against popular fluorescent proteins (GFP and YFP) and protein tags (c-MYC and HA). We have also created...

... the standard oldies but goodies (e.g., EGFP and YFP) to the new and improved fruit colors (e.g., apple...

...can be used to target any protein fused with GFP, YFP, mCerulean, or other related fluorophores. Find all...

...times brighter than the most commonly used GFPs and YFPs. Its excitation maxima is at 506 nm and its emission...from the tetrameric yellow fluorescent protein (LanYFP) from the marine invertebrate Branchiostoma lanceolatum...Institute developed mNeonGreen via 21 substitutions to LanYFP and the addition of the enhanced GFP (EGFP)-type...

...small amounts of non-specific ROS when exposed to YFP and RFP excitation wavelengths, which suggests that...

...the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free up crowded...

...C- and N-terminal tagging vectors (eg GFP, Flag, YFP, Strep, TEV-V5) with either blasticidin or puromycin...

...terminally tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (HA, FLAG)...

...works. A split FP (e.g., yellow fluorescent protein, YFP) is reunited by the interaction between two PIPs,...