...Cas9/reporter) pX458 plasmid family. They have made several modifications to the the px458 plasmid to expand...developmental studies. Key plasmid features and pX458 modifications: replacement of Cbh promoter with ...guide cassette. This process is compatible with all pX458 family plasmids. Additional Cloning Information ...Plasmid and Sequence Derivative Sources Original pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2 (...

... and a validated gRNA, based on the Zhang lab's PX458 . Multiple gRNA plasmids may be used. The HDR (homology...pFETCh_CEBPB PX458_CEBPB_1 PX458_CEBPB_2 AHR Human FLAG pFETCh_AHR PX458_AHR_1 PX458_AHR_2 ARID4B Human...pFETCh_CREB1 PX458_CREB_1 PX458_CREB_2 TGIF2 Human FLAG pFETCh_TGIF2 PX458_TGIF2_1 PX458_TGIF2_2 SSRP1 Human...pFETCh_SSRP1 PX458_SSRP1_1 PX458_SSRP1_2 ZNF146 Human FLAG pFETCh_ZNF146 PX458_ZNF146_1 PX458_ZNF146_2 ZNF580...pFETCh_ZNF219 PX458_ZNF219_1 PX458_ZNF219_2 ZNF3 Human FLAG pFETCh_ZNF3 PX458_ZNF3_1 PX458_ZNF3_2 FOXP1 Human...pFETCh_FOXP1 PX458_FOXP1_1 PX458_FOXP1_2 GATAD1 Human FLAG pFETCh_GATAD1 PX458_GATAD1_1 PX458_GATAD1_2 GATAD2A...pFETCh_GATAD2A PX458_GATAD2A_1 PX458_GATAD2A_2 HHEX Human FLAG pFETCh_HHEX PX458_HHEX_1 PX458_HHEX_2 HBP1 Human...

...selection 1 . Other constructs may be utilized, such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid...

...42230 : PX330; SpCas9 and single guide RNA 48138 : PX458; SpCas9-2A-EGFP and single guide RNA 62988 : PX459...

...48873 Mammalian BbsI yes, nick S. pyogenes Zhang PX458 (3rd Gen) 48138 Mammalian BbsI yes, cut S. pyogenes...

...TIA1 His, T7 T7 ALS Thomas Tuschl 106108 px458_2A_GFP_sgRNA_EIF2AK2 EIF2AK2 U6 VWM disease Thomas Tuschl ...