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Addgene
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  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...plasmid of 2017. Find pMD2.G. pSpCas9(BB)-2A-GFP (PX458) - This plasmid from the Zhang lab expresses Cas9... of transfected cells. Find pSpCas9(BB)-2A-GFP (PX458). lentiCRISPR v2 - This 3rd generation lentiviral...2A-GFP (PX461) - Similar to pSpCas9(BB)-2A-Puro (PX458), pSpCas9n(BB)-2A-GFP (PX461) expresses both a Cas9...

  2. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Cas9/reporter) pX458 plasmid family. They have made several modifications to the the px458 plasmid to expand...developmental studies. Key plasmid features and pX458 modifications: replacement of Cbh promoter with ...guide cassette. This process is compatible with all pX458 family plasmids. Additional Cloning Information ...Plasmid and Sequence Derivative Sources Original pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2 (...

  3. CRISPR Plasmids - Tagging

    Type
    Collection
    ... and a validated gRNA, based on the Zhang lab's PX458 . Multiple gRNA plasmids may be used. The HDR (homology...pFETCh_CEBPB PX458_CEBPB_1 PX458_CEBPB_2 AHR Human FLAG pFETCh_AHR PX458_AHR_1 PX458_AHR_2 ARID4B Human...pFETCh_CREB1 PX458_CREB_1 PX458_CREB_2 TGIF2 Human FLAG pFETCh_TGIF2 PX458_TGIF2_1 PX458_TGIF2_2 SSRP1 Human...pFETCh_SSRP1 PX458_SSRP1_1 PX458_SSRP1_2 ZNF146 Human FLAG pFETCh_ZNF146 PX458_ZNF146_1 PX458_ZNF146_2 ZNF580...pFETCh_ZNF219 PX458_ZNF219_1 PX458_ZNF219_2 ZNF3 Human FLAG pFETCh_ZNF3 PX458_ZNF3_1 PX458_ZNF3_2 FOXP1 Human...pFETCh_FOXP1 PX458_FOXP1_1 PX458_FOXP1_2 GATAD1 Human FLAG pFETCh_GATAD1 PX458_GATAD1_1 PX458_GATAD1_2 GATAD2A...pFETCh_GATAD2A PX458_GATAD2A_1 PX458_GATAD2A_2 HHEX Human FLAG pFETCh_HHEX PX458_HHEX_1 PX458_HHEX_2 HBP1 Human...

  5. Zhang Lab CRISPR Page

    Type
    Collection
    ...42230 : PX330; SpCas9 and single guide RNA 48138 : PX458; SpCas9-2A-EGFP and single guide RNA 62988 : PX459...
Showing: 1 - 7 of 7 results