...components necessary to produce an infectious viral particle (a virion) are generally divided among multiple...i.e., the genetic material enclosed in the viral particle) is delivered to a target cell upon infection....basepairs of nucleic acid can fit into a viral particle) and toxicity of the gene product (i.e., if the...can be difficult to efficiently produce viral particles). This table lists lentiviral transfer plasmids...addition of Cre turns on shRNA expression. See article for more cre-lox shRNA expression variants. Jacks...Inducible expression of transgene or shRNA. See article for detailed information about cloning & additional... a wide variety of drug selection markers. See article for more variations. Campeau 39481 pLenti-puro ...

...lentiviral particles Murine leukemia-based retroviral particles Vesicular stomatitis virus particles See Addgene's...) are packaged as replication-defective virus particles with the SARS-CoV-2 spike (S) glycoprotein on...on the envelope surface. Pseudotyped virus particles can then be used to infect permissive cells that ...infective potential. The following types of viral particles have been pseudotyped with the SARS-CoV-2 Spike...; several modifications may enhance infectious particle production, including codon optimization, deletion...Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein Nemazee lab - Pseudotyped...

...our catalog . Use the article links to find the plasmids associated with the article. We also offer ready-to-use...pHluorin Two pathways of synaptic vesicle retrieval revealed by single-vesicle imaging. Neuron. 2009 Feb 12...temporal cellular processes. Note that many of the articles linked below include variants with different binding...viral service icon . Metal Ions Target Description Article PI Calcium jGCaMP8 Fast Genetically Encoded Calcium...Return to Top Small Molecules Target Description Article PI Adenosine Express the adenosine sensor GRAB_...Return to Top Neurotransmitters Target Description Article PI Acetylcholine Family of ACh sensors (GACh) based...plasma membrane Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff...

...selection cassette in format: PGK-rtTA-2A-puro; see article for tagged insert options rtTA On Root 11651 pLVUT-tTR-KRAB...inducible expression of transgene or shRNA; see article for detailed information about cloning and additional...selection cassette in format: rtTA3+IRES+Neo; see article for additional selection options third-generation...pInducer20 Lentiviral vector for shRNA expression; see article for additional tookit plasmids third-generation...transposon system; has luciferase in cloning site; see article for additional selection and FP option rtTA On ...contains expression cassette for TetR-P2A-GFP; see article for additional constructs TetR On Geley Transactivators... rtTA driven by retroviral LTR promoters ; see article for lentiviral vectors for tet-inducible gene or...

...spike protein mutations. Research Articles : A list of free access articles on COVID-19 research. Protocols... Resources Research Articles Many publishers have allowed free access to articles related to SARS-CoV-..., we have linked to collections of open-access articles, protocols, and other resource collections related...Collection Springer Nature SARS-CoV-2 and COVID-19 Articles Wiley Online Library - Covid-19: Novel Coronavirus...Free to Access Collections of COVID-19 related articles spanning multiple publishers can be found here...

...vectors consist of full particles (white arrowhead) relative to empty particles (black arrowhead). Scale...produced your viral vector lot. At Addgene, AAV particles are titered by droplet digital PCR (ddPCR) using...of empty to full (i.e., genome containing) AAV particles within representative vector preparations was ...microscopy after negative staining. Empty vector particles can be identified after negative staining and ...and appear darker than full vector particles. Figure 3: Electron micrograph of AAV vector preparation shows...

...Clathrin-coated vesicles Clathrin light chain GFP Bo Huang 54040 mEmerald-Clathrin-15 Clathrin-coated vesicles Clathrin...then are transported to the Golgi apparatus in vesicles to be packaged and shipped. Many of the proteins...EGFP Toren Finkel 14435 mRFP-Clc Clathrin-coated vesicles Clathrin, light polypeptide mRFP Ari Helenius ...Davidson 79997 pmiRFP703-Clathrin Clathrin-coated vesicles Clathrin miRFP703 Vladislav Verkhusha 21741 Clathrin-LCa-EYFP...Clathrin-LCa-EYFP Clathrin-coated vesicles Clathrin, light polypeptide EYFP Xiaowei Zhuang 14437 mRFP-...

...Plasmids can be found associated with the following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila...N-terminal epitope tags. The plasmids in the following articles provide PCR templates for amplification of the...can be found associated with the following two articles: Kunzelmann, et al. G3 2016 Bottcher, et al. Nucleic...Plasmids can be found associated with the following article: Paix, et al. Genetics 2014 Allen Institute for...Institute for Cell Science has produced the first publicly available collection of fluorescently tagged, ...

... the experiment as described in the associated article (listed below by PubMed ID). These factors include...26028531 Huangfu OCT4 H. sapiens multiple, see article 69537 activate S. pyogenes 26352799 Otonkoski AAVS1...25336740 Christiaen EGFP A. victoria multiple, see article 60071 dCas9-FokI S. pyogenes 24770325 Joung EGFP... Kras, p53, and Lkb1 M. musculus multiple, see article 60224 cut S. pyogenes 25263330 Zhang LacZ E. coli...23929340 Kamoun PDS1 N. benthamiana multiple, see article 49772 cut S. pyogenes 24112467 Kamoun PDS3 A. thaliana...pyogenes 26944679 Shaw Please refer to the primary article to confirm details and outcomes. Submit Validated...

...Lentiviruses are distributed as purified viral particles in solution that are suitable for in vivo use....Barcode Library Version 1 Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding library...Barcode Library Version 2 Ready-to-use lentiviral particles carrying version 2 of the CellTag barcoding library...Barcode Library Version 3 Ready-to-use lentiviral particles carrying version 3 of the CellTag barcoding library...

...transgenic mice. ID Plasmid/Article Description Expression System PI See article Brainbow constructs Cre-...UBC-Cre25nt Cre with a 25 nucleotide extracellular vesicle targeting sequence and CFP UBC Mammalian van Rheenen...activates your gene fused to eGFP, removes dsRed. See article for other similar plasmids. Retroviral Clevers ...with multiple distinct colors Mammalian Sanes See article Multibow constructs Multibow uses a binary code...vector, Cre dependent tdtomato expression. See article for more colors. Mammalian Zeng 24334 hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean...

...which enable the production of infectious AAV particles without the use of a helper virus. Instead, specific...produce AAV. In order to generate infectious AAV particles, a helper plasmid (encoding adenovirus E4, E2A...and/or can be used to generate infectious AAV particles. Additional helper plasmids (which may not be ...Addgene) may be required to produce infectious AAV particles. You can search or sort this table based on plasmid...

...select the article link to find more information. Delivery Method Species Description Article PI Lentivirus...the article link to find more information. Convert From Convert To Delivery Method Species Article PI ...

... Le Cong, the first author of a seminal CRISPR article from Dr. Feng Zhang's Lab, has assembled a list...Le Cong, the first author of one of the seminal articles from Dr. Feng Zhang's Lab ( Science , February...the Zhang lab has published this Nature Protocol article . Should I add the PAM sequence to the oligo when...

...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...publication link to view all other plasmids from that article. Click on the PI link to view all available plasmids...

...about the repression system can be found in this article: Vigouroux, A., Oldewurtel, E., Cui, L., Bikard...chromosomal integration of pLC97 can be found in this article: St-Pierre, F., Cui, L., Priest, D. G., Endy, D...

...distributed as 100 µL solutions of purified viral particles that are suitable for in vivo use. Order a high-quality... examples of quality control data. Help Center Articles Additional details about viral vector production...

...mammalian packaging cell lines to generate adenovirus particles. Read our Adenoviral Guide for more information...pShuttle-CMV plasmid with Gateway cassette; See article for additional plasmids Zhang 50957 RedTrackCMV...

...control). The end result of the meeting will be a publicly shared practical guide for all scientists in the...have final approval before anything is released publicly, but please only share materials that you are ...

...provide the necessary components to make lentiviral particles (for details about lentivirus, see our Lentivirus... AAV transfer vector and used to generate AAV particles (for details, see our AAV Guide ) ∼4.5 kb packaging...