...your article doesn’t turn into a textbook. Get the journal’s submission rules for review articles Whether...from the article to be able to keep editing it effectively. You will also begin to hate the article. This...Writing a review article is a wonderful way to develop and exercise your scientist skill set. If you ...basically, a scientist’s tool kit. I wrote a review article on histone modifers in B and T cells with my adviser... good” by no definitive criteria. I read these articles to get a sense of the themes in the field and ...for seminal papers on these themes, and also for articles from the active/well-known researchers in the ...feedback on the scope and the content. Submit your article! Overall, writing a review can be overwhelming ...

...of citing articles. From the article list you can click on the PubMed link to find the article abstract...from these citing articles. What's this? Simply put, if the authors of a "Citing Article" have deposited... a new feature on our plasmid pages - links to articles citing this plasmid. Now you can learn how a plasmid...publications, you'll find a link to the list of citing articles under the "Resource Information" heading in the...heading, you'll be directed to a page listing the articles that cite this plasmid. You can use the dropdown...deposited plasmids associated with this "Citing Article" - such as cloning their gene of interest into a previously...from Addgene may get your paper listed in the "Articles Citing This Plasmid" list increasing your work's...

...Lentiviral Particles E.1 Recommended materials E.2 Protocol for producing lentiviral particles F. Infecting...for producing viral particles. pMD2.G Envelope plasmid for producing viral particles. Note: pLKO.1 can ...selection G. Safety H. References H.1 Published articles H.2 Web resources I. Appendix I.1 Sequence of ...transfection, or can be converted into lentiviral particles for subsequent infection of a target cell line...sequence. Back to Top E. Producing Lentiviral Particles Before this step, you must contact your institution...Addgene recommends that you generate lentiviral particles and infect the target cells. Addition of puromycin...Tools page. E.2 Protocol for Producing Lentiviral Particles This protocol is for transfection in a 6 cm plate...

...B): LiCl selectively precipitates RNA versus DNA or proteins. Add the correct amount of 7.5 M LiCl solution...solution to bring the concentration of LiCl in the extracted aqueous layer to 2.5 M. Incubate at -20°C ...

...for the presence of particles still in solution. Continue to stir until all particles have dissolved. This...stir on a magnetic stir plate. Stir until all particles have dissolved. Note: The powder should dissolve...

...containing) particles. On average, iodixanol-purified AAV preparations contain ~20% empty particles. 60% iodixanol...

...proteins, cell fragments, salt and other extra particles from solutions. Supernatant contains the plasmid...may appear, consisting of precipitated protein particles Bottom phase - Organic phase (protein) Pipet the...

...too many cells relative to the number of virus particles reduces the transduction efficiency, resulting... so many that they vastly outnumber the virus particles. Incubate the cells with the virus for 48–72 h...

...Determine the number of genome-containing particles in an AAV prep using qPCR, SYBR Green Technology,... to determine the number of genome-containing particles of an AAV prep using SYBR green technology. This...

...generally represents the number of infectious viral particles. Users may need to run lower or higher dilutions... zero (A01). In this protocol, the lentiviral particles are serially diluted and used to transduce HEK293T...

...components necessary to produce an infectious viral particle (a virion) are generally divided among multiple...i.e., the genetic material enclosed in the viral particle) is delivered to a target cell upon infection....basepairs of nucleic acid can fit into a viral particle) and toxicity of the gene product (i.e., if the...can be difficult to efficiently produce viral particles). This table lists lentiviral transfer plasmids...addition of Cre turns on shRNA expression. See article for more cre-lox shRNA expression variants. Jacks...Inducible expression of transgene or shRNA. See article for detailed information about cloning & additional... a wide variety of drug selection markers. See article for more variations. Campeau 39481 pLenti-puro ...

...lentiviral particles Murine leukemia-based retroviral particles Vesicular stomatitis virus particles See Addgene's...) are packaged as replication-defective virus particles with the SARS-CoV-2 spike (S) glycoprotein on...on the envelope surface. Pseudotyped virus particles can then be used to infect permissive cells that ...infective potential. The following types of viral particles have been pseudotyped with the SARS-CoV-2 Spike...; several modifications may enhance infectious particle production, including codon optimization, deletion...Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein Nemazee lab - Pseudotyped...

...our catalog . Use the article links to find the plasmids associated with the article. We also offer ready-to-use...pHluorin Two pathways of synaptic vesicle retrieval revealed by single-vesicle imaging. Neuron. 2009 Feb 12...temporal cellular processes. Note that many of the articles linked below include variants with different binding...viral service icon . Metal Ions Target Description Article PI Calcium jGCaMP8 Fast Genetically Encoded Calcium...Return to Top Small Molecules Target Description Article PI Adenosine Express the adenosine sensor GRAB_...Return to Top Neurotransmitters Target Description Article PI Acetylcholine Family of ACh sensors (GACh) based...plasma membrane Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff...

...selection cassette in format: PGK-rtTA-2A-puro; see article for tagged insert options rtTA On Root 11651 pLVUT-tTR-KRAB...inducible expression of transgene or shRNA; see article for detailed information about cloning and additional...selection cassette in format: rtTA3+IRES+Neo; see article for additional selection options third-generation...pInducer20 Lentiviral vector for shRNA expression; see article for additional tookit plasmids third-generation...transposon system; has luciferase in cloning site; see article for additional selection and FP option rtTA On ...contains expression cassette for TetR-P2A-GFP; see article for additional constructs TetR On Geley Transactivators... rtTA driven by retroviral LTR promoters ; see article for lentiviral vectors for tet-inducible gene or...

...spike protein mutations. Research Articles : A list of free access articles on COVID-19 research. Protocols... Resources Research Articles Many publishers have allowed free access to articles related to SARS-CoV-..., we have linked to collections of open-access articles, protocols, and other resource collections related...Collection Springer Nature SARS-CoV-2 and COVID-19 Articles Wiley Online Library - Covid-19: Novel Coronavirus...Free to Access Collections of COVID-19 related articles spanning multiple publishers can be found here...

...vectors consist of full particles (white arrowhead) relative to empty particles (black arrowhead). Scale...produced your viral vector lot. At Addgene, AAV particles are titered by droplet digital PCR (ddPCR) using...of empty to full (i.e., genome containing) AAV particles within representative vector preparations was ...microscopy after negative staining. Empty vector particles can be identified after negative staining and ...and appear darker than full vector particles. Figure 3: Electron micrograph of AAV vector preparation shows...

...Clathrin-coated vesicles Clathrin light chain GFP Bo Huang 54040 mEmerald-Clathrin-15 Clathrin-coated vesicles Clathrin...then are transported to the Golgi apparatus in vesicles to be packaged and shipped. Many of the proteins...EGFP Toren Finkel 14435 mRFP-Clc Clathrin-coated vesicles Clathrin, light polypeptide mRFP Ari Helenius ...Davidson 79997 pmiRFP703-Clathrin Clathrin-coated vesicles Clathrin miRFP703 Vladislav Verkhusha 21741 Clathrin-LCa-EYFP...Clathrin-LCa-EYFP Clathrin-coated vesicles Clathrin, light polypeptide EYFP Xiaowei Zhuang 14437 mRFP-...

...Plasmids can be found associated with the following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila...N-terminal epitope tags. The plasmids in the following articles provide PCR templates for amplification of the...can be found associated with the following two articles: Kunzelmann, et al. G3 2016 Bottcher, et al. Nucleic...Plasmids can be found associated with the following article: Paix, et al. Genetics 2014 Allen Institute for...Institute for Cell Science has produced the first publicly available collection of fluorescently tagged, ...

... the experiment as described in the associated article (listed below by PubMed ID). These factors include...26028531 Huangfu OCT4 H. sapiens multiple, see article 69537 activate S. pyogenes 26352799 Otonkoski AAVS1...25336740 Christiaen EGFP A. victoria multiple, see article 60071 dCas9-FokI S. pyogenes 24770325 Joung EGFP... Kras, p53, and Lkb1 M. musculus multiple, see article 60224 cut S. pyogenes 25263330 Zhang LacZ E. coli...23929340 Kamoun PDS1 N. benthamiana multiple, see article 49772 cut S. pyogenes 24112467 Kamoun PDS3 A. thaliana...pyogenes 26944679 Shaw Please refer to the primary article to confirm details and outcomes. Submit Validated...

...Lentiviruses are distributed as purified viral particles in solution that are suitable for in vivo use....Barcode Library Version 1 Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding library...Barcode Library Version 2 Ready-to-use lentiviral particles carrying version 2 of the CellTag barcoding library...Barcode Library Version 3 Ready-to-use lentiviral particles carrying version 3 of the CellTag barcoding library...