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Showing: 1 - 20 of 37 results
  1. CRISPR Fly Design - Drosophila genome engineering

    Type
    Collection
    ...an open science genome engineering project in Drosophila. ...fly design is a genome engineering project in Drosophila, consisting of transgenic cas9 fly lines and ...1gRNA expression of gRNA under control of the Drosophila U6:1 promoter Bullock 49409 pCFD2-dU6:2gRNA expression... expression of gRNA under control of the Drosophila U6:2 promoter Bullock 49410 pCFD3-dU6:3gRNA expression...expression of gRNA under control of the Drosophila U6:3 promoter Bullock 49411 pCFD4-U6:1_U6:3tandemgRNAs ...3tandemgRNAs expression of two gRNAs from Drosophila U6:1 and U6:3 Bullock 73914 pCFD5 multiplex gRNA plasmid Bullock...SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster. Bullock 62208 pnos-Cas9-nos Expresses...
  2. FlyCRISPR

    Type
    Collection
    ...Browse CRISPR Cas9 gRNA plasmids for drosophila research from the O'Connor-Giles, Wildonger, and Harrison...modifications can be efficiently generated in Drosophila and transmitted through the germline. Using an...plasmids are described in: Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Gratz...codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter. 45945 pHsp70-Cas9 : The codon-optimized...codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). ...expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter. 51019 pDsRed-attP : Vector...
  3. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...pCFD3-NS Drosophila Drosophila U6:3 pegRNA BbsI No Vermilion Norbert Perrimon pCFD5-NS Drosophila Drosophila...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...expression in mammalian systems, bacteria, plants, and Drosophila. Mammalian ID Plasmid Gene/Insert Promoter Selectable...Insert Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker...
  4. CRISPR Plasmids - Drosophila

    Type
    Collection
    ... CRISPR Drosophila CRISPR Plasmids: Drosophila Browse CRISPR...designed for use in Drosophila and other insects. CRISPR...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...CRISPR plasmids have been designed for use in Drosophila and other insects. Cut Fully functional CRISPR...
  5. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...systems and Drosophila. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid ...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...
  6. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...for expression in mammalian systems, bacteria, Drosophila, plants, and yeast. Mammalian Plasmid Gene/Insert...Bacteria Plasmid Gene/Insert Promoter PI Publication Drosophila Plasmid Gene/Insert Promoter PI Publication Plant...
  7. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...for expression in mammalian systems, bacteria, Drosophila, plants, and yeast. Mammalian Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Drosophila Plasmid Gene/Insert Promoter Selectable Marker...
  8. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...for expression in mammalian systems, bacteria, Drosophila, plants, C. elegans, yeast, zebrafish, and Xenopus...Bacteria Plasmid Gene/Insert Promoter PI Publication Drosophila Plasmid Gene/Insert Promoter Selectable Marker...
  9. CRISPR Plasmids - Tagging

    Type
    Collection
    ... or C-terminal tagging in Drosophila cells. N terminal tagging in Drosophila cells 3.2 MB C terminal tagging...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR... Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System The Förstemann lab has developed...developed a CRISPR tagging technique for use in Drosophila cells that uses PCR to generate both an expression... tagging in Drosophila cells 3.3 MB Seydoux C. elegans Tagging System Geraldine Seydoux's lab has developed...
  10. CRISPR References and Information

    Type
    Collection
    ...checks for off-target binding. Currently supports: Drosophila, Arabidopsis, zebrafish, C. elegans, mouse, human...tropicalis and X. laevis), zebrafish, sea squirt, Drosophila, C. elegans, Arabidopsis, rice, sorghum, silkworm...checks for off-target binding. Currently supports: Drosophila, Arabidopsis, zebrafish, C. elegans , mouse, ...Group (moderated by Feng Zhang lab): Forum/FAQ Drosophila: General Advice and Forum/FAQ CRISPR Blog Topic...
  11. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...49410 Drosophila BbsI none S. pyogenes Virmilion Bullock and Port pAc-sgRNA-Cas9 49330 Drosophila BspQI...49411 Drosophila BbsI none S. pyogenes Virmilion Bullock and Port pU6-BbsI-chiRNA 45946 Drosophila BbsI...:3tandemgRNAs Drosophila Single plasmid for the expression of two gRNAs from Drosophila U6:1 and U6:3 ...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...system , eg. Mammalian, Lentiviral, AAV, Bacteria, Drosophila, Yeast, Plant, Xenopus, Worm, or Other. Whether...3 promoters. Bullock pCFD5 Drosophila Utilizes endogenous tRNA processing machinery to excise multiple...
  12. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...
  13. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...
  14. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...
  15. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...
  16. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...
  17. CRISPR Plasmids - Parasites

    Type
    Collection
    ...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...
Showing: 1 - 20 of 37 results