...Recombination in Drosophila Learn about Cre-Lox Resources on Addgene.org Find Drosophila Plamids Drosophila CRISPR... ortholog in the Drosophila genome? Continue reading and I’ll show you how Drosophila can be used to push...favorite gene (YFG) in Drosophila (and even if there’s not!) the wealth of Drosophila genetic tools available..., and overexpress YFG in a Drosophila cell line. Knock downs in Drosophila cell lines are extremely effective...your research in new and exciting directions. Drosophila are very easy to manipulate genetically and have... second post will detail a major tool used by Drosophila geneticists (the Gal4/UAS system), and the third... how you can make your own mutant flies. Find Drosophila Resources at Addgene The birds and the bees ...

...earlier, even genes not found in Drosophila can be studied. Using a Drosophila cell line, I’ve studied a transcription...described the fundamentals of how to work with Drosophila as an experimental model organism. I then described...your flies will be ready for experimentation. Drosophila are amenable to many different kinds of experimental...can be employed to look at oxygen consumption. Drosophila are also used in cancer research (3). The sky..., which flies do not have!) (4). The power of Drosophila as a model organism is in its amenability to ...with flies, you can utilize the resources of the Drosophila community to easily test your hypotheses. Many...had nearly a decade of experience working with Drosophila as an experimental system. Jon enjoys studying...

...this enzyme in Drosophila. If you're still reading this post and you don't work in Drosophila, you may be...phenotype. This problem is especially stark in Drosophila, where expression of Cre from the standard UAS...looking to use site-specific recombination in Drosophila, read on to learn about new recombinases suitable...sought to make complex genome modifications in Drosophila using multiple recombinases. To make multiple...Saccharomyces cerevisiae) had been used successfully in Drosophila, so Rubin sought to test the suitability of four...recombinases KD, B2, B3 and FLP can be used together in Drosophila as a toolkit for specific recombination patterns...further enhance genome editing capabilities in Drosophila. References: Multiple new site-specific recombinases...

...second post in our quick guide to working with Drosophila, you’ll learn how to maniupate expression of ...be overexpressed or knocked down. To do this, Drosophila geneticists use the Gal4/UAS system. This incredibly...activation system co-opted from yeast (1). It is a Drosophila geneticist’s main workhorse to turn genes on ...had nearly a decade of experience working with Drosophila as an experimental system. Jon enjoys studying...Roman, R. L. Davis, Gene expression systems in Drosophila: A synthesis of time and space, Trends Genet.... : A Versatile Toolkit for Gene Expression in Drosophila, 3, 1–10 (2015). PubMed PMID: 21356876. 5. J....tools for gene expression and clonal analysis in Drosophila, 9, 47–55 (2012). PubMed PMID: 22205518. PubMed...

...Wiechaus first identified the hedgehog gene in Drosophila. They observed that flies with non-functional...denticles (bristles) outline the segments of the Drosophila embryo. In flies with non-functional hedgehog...blood vessels). Spaghetti squash (Sqh) is the Drosophila Regulatory Light Chain (RMLC) of nonmuscle myosin... Monster. cookie monster (comr) is actually a Drosophila meiotic arrest gene that is necessary for spermatogenesis...cell fate of the dorsal mesoderm in developing Drosophila embryos (Azplazu and Frasch, 1993).  Tinman, ...determine cell fates in the dorsal mesoderm of Drosophila." Genes & Development 7.7b (1993): 1325-1340....specification of the heart and visceral muscles in Drosophila." Development118.3 (1993): 719-729. PubMed PMID...

.... So far, tools to overcome this challenge in Drosophila require heat-shocking the system or using chemical...techniques to reliably label any post-mitotic cells in Drosophila. SPARC was developed by Thomas Clandinin’s lab... incorporates several common genetic tools in Drosophila (Fig. 1):  GAL4-UAS, a transcription factor ...labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe. Image from Isaacman-Beck et al., ...binding site and effector in the construct. In Drosophila, the SPARC systems can be used both for visualization...tools for gene expression and clonal analysis in Drosophila. Nature Methods 9:47–55 . https://doi.org/10.1038...

...culture. Repressible Binary Systems GAL4/UAS In Drosophila or development studies, you may hear some transcription...increase QUAS driven expression by ~3,300-fold in Drosophila cells and ~24,000-fold in human HeLa cells as... reversible by the addition of quinic acid to Drosophila cells. Work by Wei et al. from Kang Shen’s lab...used together for complex genetic analysis in Drosophila or for precise control over multiple genes in...Refinement of Tools for Targeted Gene Expression in Drosophila.” Genetics 186(2) (2010): 735-55. PubMed PMID...tools for gene expression and clonal analysis in Drosophila.” Nat Methods 9(1) (2011): 47-55. PubMed PMID...mosaic with dual binary transcription systems in Drosophila.” Nat Neurosci 9(5) (2006): 703-9. PubMed PMID...

... The fruit fly (Drosophila melanogaster) Another popular model organism is Drosophila melanogaster, or... Hunt Morgan, who is known as the “father” of Drosophila research. Morgan discovered that genes were found...Central PMCID: PMC3703927. Jennings, Barbara H. "Drosophila–a versatile model in biology & medicine." Materials... Charles D. Nichols. "Human disease models in Drosophila melanogaster and the role of the fly in therapeutic...Refinement of tools for targeted gene expression in Drosophila." Genetics 186.2 (2010): 735-755. PubMed PMID... on the Addgene blog Read our quick guide to Drosophila Download the fluorescent proteins 101 eBook Read...

...Labeling Drosophila neurons with dBrainbow and Flybow Based on the Brainbow 1.0 system, Drosophila Brainbow...Brainbow (dBrainbow; Hampel et al., 2011) labels Drosophila neurons at the neuroblast stage, meaning that ...Similarly, Flybow adapts Brainbow 2.0 for use in Drosophila melanogaster and is under the control of Flp ... cell labeling for neural circuit analysis in Drosophila melanogaster." Nature methods 8.3 (2011): 260...

...study model organisms such as Drosophila and zebrafish. Brainbow in Drosophila has aided in the mapping of...tracing in other systems, such as zebrafish and Drosophila. Why Brainbow? Brainbow highlights the trend ...Clevers H. Cell. 2010 Oct 1;143(1):134-44. PubMed. Drosophila Brainbow: a recombinase-based fluorescence labeling... cell labeling for neural circuit analysis in Drosophila melanogaster. Hadjieconomou D, Rotkopf S, Alexandre...

...other model systems, including E. coli, plants, Drosophila, and zebrafish!   Why use multiplexed gRNAs?...restriction enzyme BsaI. If you’re working in Drosophila, a two-gRNA expressing plasmid is available from...supplied on a separate plasmid. Multiplexing in Drosophila Bullock Lab Multiplex Plasmid: Two gRNAs can ...or SLIC cloning. gRNAs are expressed from two Drosophila U6 promoters. Cas9 must be supplied on a separate...efficient germline and somatic genome engineering in Drosophila.” Proceedings of the National Academy of Sciences...

... a set of cytochrome P450 genes discovered in Drosophila melanogaster (Gilbert, 2004). Mutations in these... that mediate steroid hormone biosynthesis in Drosophila melanogaster." Molecular and cellular endocrinology...

...simple technique has been shown to work in plant, Drosophila and mammalian cells, and it may find wide use...rapidly and efficiently photoconverted in plant, Drosophila, and mammalian cells. Photoconverted GFP is compatible...

... from Drosophila Actin 5c gene Constitutive  Commonly used in expression systems for Drosophila. Polyhedrin...containing Gal4 binding sites, commonly used in Drosophila Specific Requires the presence of Gal4 gene to...

...tissues or organs in whole animal models like Drosophila melanogaster, obtaining enough material for a...elements is controlled during development using the Drosophila wing as a model. A typical ChIP-Seq experiment...

...sensors in experiments The Li lab used GRABDA in drosophila, zebrafish, and mouse brain slices and freely...Demonstrate DA detection in a single neuron in vivo in Drosophila Detect DA dynamics in response to a threatening...

...used in many cell types and organisms including Drosophila, fish, and mice, by replacing the effector with...labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe. Image from Isaacman-Beck et al., ...

...apoptosis in HeLa cells, zebrafish embryos, and Drosophila. They treated HeLa cells expressing FlipGFP with...visualize apoptosis of enterocytes in the midgut of Drosophila, an observation that has been difficult until...

...FLIS and Flies  Drosophila, more commonly known as fruit flies, have been an important animal model for...

... can't reproduce with wild ones. For example, Drosophila lines with compound autosomes are completely ...Chromosome Replacement by Compound Autosomes in Drosophila melanogaster. Genetics 74, 461–475 (1973). PubMed...