...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...double stranded breaks. Cytosine base editors are created by fusing Cas9 nickase or catalytically inactive...converted to thymidine through base excision repair, creating a C to T change (or a G to A on the opposite strand..., which is treated like guanosine by the cell, creating an A to G (or T to C) change. Adenine DNA deaminases...deaminases do not exist in nature, but have been created by directed evolution of the Escherichia coli TadA...evolved TadA domain is fused to a Cas9 protein to create the adenine base editor. Both types of base editors...adjust the editing window, or adding fusions that increase product purity such as the DNA glycosylase inhibitor...

...In a CRISPR screening experiment, target cells are treated with the pooled library to create a population...mutant cells that are then screened for a phenotype of interest. Screening experiments using a pooled ...CRISPR pooled libraries for large-scale functional screens. Available for knockout, activation, inhibition...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...Blog Posts CRISPR is a useful tool for genetic screening experiments, due to the relative ease of designing...libraries and keep the following in mind: CRISPR screening experiments require electroporation to amplify...NGS) to confirm library representation. CRISPR screening experiments require large quantities of cells ...

...extracellular regions of cell surface receptor and secreted proteins as soluble recombinant molecules....extracellular regions of cell surface receptor and secreted proteins as soluble recombinant molecules. The...typically used in systematic protein interaction screens or cellular assays to identify novel interactions...Laboratories interested in these approaches have created this resource to facilitate open and free exchange...AVEXIS (for Avidity-based extracellular interaction screen) in which the extracellular regions of receptor...proteins P. falciparum sporozoite cell surface and secreted proteins Mouse sperm Mouse egg Laboratories using...description: Avidity based Extracellular Interaction Screening (AVEXIS) for the scalable detection of low affinity...

...with work directed towards increasing on-target editing rates and decreasing off-target editing rates. ...others. Homology-directed repair (HDR) allows us to create a variety of modifications in endogenous loci with... locus makes it especially amenable to genetic screens. Pooled gRNA libraries can be used to identify ...sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology... : Base editors directly convert bases without creating a double-strand break. This method displays higher...method targets RNA rather than DNA, it does not create permanent genome edits or double-strand breaks....epigenetic modifiers can modulate transcriptions and create heritable epigenetic marks. Visualize : Fluorescently...

...reprogramming of adult pancreatic exocrine cells to beta-cells. Nature. 2008 Aug 27. Melton Pancreatic Acinar Cells...human cells in 2006 and 2007, respectively. To create iPSCs, fully-differentiated adult somatic cells...Plasmids in the table below. Once iPSCs have been created they can be directly differentiated into specific..., and transdifferentiation utilize plasmids to increase expression of key factors. The tables below will...plasmids available from Addgene that can be used to create iPSCs or another cell type. You can also search...iNDI) Collection for plasmids that can be used to create cell lines with endogenously-tagged gene variants...Plasmids Browse the table below for plasmids used to create induced pluripotent stem cells (iPSCs). Sort the...

... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite strand) mutations...hypermutation in a 100 bp window. This technique creates diverse populations of mutants for directed evolution...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...

... cell cultures using hybridomas, cell cultures created by fusing B cells with myeloma cells (immortalized...immortalized B cells). The genetic reorganization that creates variety in the antibody allowing for their extreme...animals immunized as described above are used to create hybridomas, which produce large amounts of a single...recombinant antibodies. Recombinant antibodies are created by cloning antibody genes from specific hybridoma...goes through a randomized reorganization process, creating a unique binding site. This process, known as ...roles in the immune response, and can be used to increase flexibility or specificity in antibody-based methods... Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite strand) mutations...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...

...synthetically produced recombinant antibodies, created by cloning antibody components into plasmids and...mammalian tissue culture cells, the R-mAbs are secreted into the culture media and can be collected for...smaller fragment of an antibody, which could offer increased stability and ease of production due to their ...highlights plasmid collections that can be used to create and express plasmid-based antibodies. Description...domains to obtain recombinant hybridomas, which secrete antibodies as Fab' fragments, isotype-switched ...antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount immunostaining...

...Mammalian Expression HcRed1 588 618 0.3 Dimer pCAG-HcRed - Mammalian Expression HcRed1-N1 - Mammalian Expression...backbones with different fluorescent tags for you to create fusion proteins with your gene of interest. Special...backbones with different fluorescent tags for you to create fusion proteins with your gene of interest. These...: A database for users of fluorescent proteins created by Talley Lambert at Harvard Medical School More...Expression HcRed1-C1 - Mammalian Expression dKatushka 588 635 22 5.5 Dimer dKatushka-pBAD - Bacterial Expression... Center at UCSF / QB3 Wiki FPbase : A database created by Talley Lambert at Harvard Medical School Zeiss...

... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite strand) mutations...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...

...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite strand) mutations...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...

...factor isoform in pooled screens. This collection and library can be used for screening transcription factors...available at Addgene. The collection can be used for screening transcription factors for cellular programming....026 . TF ORF barcode PCR for NGS QC and bulk screening (85 KB) TF ORF barcode dial out PCR for 10X scRNA-seq...bp sequence, to simplify identification during screening. Library ID PI Description MORF library 137000...

...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...

...Return to top Secretory Pathway The secretory pathway is named for its role in secreting proteins from...Posts Background General Cytoskeletal Junctions Secretory Pathway Background The subcellular localization... shipped. Many of the proteins that enter the secretory pathway require chaperones to help with folding... or search the table below to find markers of secretory pathway components. ID Plasmid Structure Targeting...

...tetracycline-controlled transactivator (tTA) was developed. tTA was created by fusing tetR with the C-terminal domain of VP16...tTA binding to the tetO sequences and subsequent increase in expression by the activation domain, resulting...repressor, or rTetR, which reversed the phenotype and created a reliance on the presence of tetracycline for ... etracycline-controlled t rans a ctivator) was created by fusing rTetR with VP16. The tetracycline on ...interest with Strep-Tag and Tet-On 3G transactivator, creating an auto-regulated Tet-On 3G System. Contains homology...

...carcinoid, pancreatic, and related neuroendocrine cancers. The NETRF believes that creating and maintaining... accelerate scientific discovery that will help create new and more effective therapies for these tumors...States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine Tumors...

...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

... for expressing secreted recombinant proteins from human erythrocytes that was created for systematic ...receptor proteins for use with AVEXIS interaction screening Special...systematic screening of extracellular protein interactions to identify novel receptor-ligand interactions that ...

...CRISPR plasmids that create double strand breaks in DNA....Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...