...targeted neuronal manipulation. Topics may include: How this technology is used for targeted neuronal manipulation...Series AAV Technologies and Methods for Targeted Neuronal Manipulation Meeting Date September 21, 2018 Contact... AAV-based molecular tools to achieve targeted neuronal manipulation (aka intersectional control). The...fit in the big picture of methods for targeted neuronal manipulation? Best practices for using this techonology... is presenting on) is used to achieve targeted neuronal manipulation. Figures, tables, data, or images...communication representing a reference for AAV use in neuronal intersectional control. Through your participation...Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation. Haery L, Deverman BE, Matho KS, ...

...expression Tyler Jacks 11586 pSico PGK puro Cre addition causes the puromycin gene to be recombined out of the...expression Tyler Jacks 12084 pSicoR PGK Puro Cre addition causes both puromycin and shRNA to be recombined out ...PI 8452 pMKO.1 puro Retroviral vector for shRNA expression Bob Weinberg 10675 pMKO.1 puro GFP shRNA Negative...pMKO.1 puro; Contains shRNA against GFP William Hahn 10676 pMKO.1 GFP Derivative of pMKO.1 puro with GFP... GFP instead of puromycin resistance gene William Hahn Additional Resources Web References Read our detailed...

...targeting efficiency. SpCas9 and SpCas9n with 2a-Puro and 2a-EGFP are also available. 2. SpCas9 (or SpCas9n...2A-EGFP and single guide RNA 62988 : PX459; SpCas9-2A-Puro and single guide RNA 48873 : PX460; SpCas9n (D10A...) and single guide RNA 62987 : PX462; SpCas9n-2A-Puro (D10A nickase) and single guide RNA 48137 : PX165...2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse the Addgene GeCKO Library CRISPR ...PX551; Truncated MeCP2 promoter-driven SpCas9; for neuronal expression 60958 : PX552; U6 promoter-driven; ...pAAV-pMecp2-SpCas9-spA) for expressing Cas9 in neurons. SpCas9 guide acceptor: The PX552 plasmid (#60958...-mediated expression of an sgRNA targeting the neuronal-specific gene NeuN. As a control they designed...

...collection of Armamentarium tools will aid neuroscientists in studying circuits in laboratory animals ...technologies have the potential to transform neuroscience research by bringing the precision of molecular...rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core has 100% seq conservation...BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq applications...vector for Cre expression in forebrain GABAergic interneurons. For use in combination with Cre-dependent transgenic...

...inflammation and infection, regeneration, human neurological disorders, microbiota studies, and drug discovery...Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders - Nicholas Cole Lab. A kit consisting...production of transgenic zebrafish for studying neurodegenerative disorders. Fluorescent Protein Fusion Construct...resources and developing new lines and technology. European Zebrafish Resource Center (EZRC) - A repository...) for the distribution of zebrafish lines from European researchers. International Zebrafish Society (...

... shRNA; neomycin selection; plasmid 21915 has puromycin selection TetR On Wiederschain 41393 pCW57.1 Lentiviral...cloning; selection cassette in format: PGK-rtTA-2A-puro; see article for tagged insert options rtTA On Root...from pCW57.1. rtTA was replaced with tTA, and Puromycin was replaced with Blasticidin selection. Please... interest. tTA Off Sabatini 92099 AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep Bidirectional promoter controls expression....tTA Adenoviral; CMV promoter; see article for neuronal-specific promoter and enhancer options tTA Off...

...specific neurons, often utilizing viral expression systems, to activate or deactivate these neurons in a .... When using these opsins as a tool to control neuronal activity, scientists generally attach a fluorescent... table below for the optogenetics plasmids for neuronal excitation and inhibition. For example, search...

...pPBT-peRNA_GG-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob Giehm Mikkelsen pPBT-PE2-PuroTK-pegRNA_GG...piggyBac hU6 pegRNA BsmBI Yes (Cas9 H840A + MMLV RT) PuroTK Jacob Giehm Mikkelsen pU6-tevopreq1-GG-acceptor...

...3xFNLDD/pMXs-puro iChIP plasmid, expresses 3xFNLDD in mammalian cells 51240 3xFLAG-dCas9/pMXs-puro Expresses...recognizing a telomere repeat 63590 3xFN-Tel-TAL/pMXs-puro Expresses a 3xFLAG-tagged TAL protein recognizing...repeat. A retroviral expression vector with the puromycin resistance gene 63591 3xFN-Tel-TAL/pMXs-neo Expresses...

...tropism - AAV.MaCPNS1, AAV.MaCPNS2 Cell type-biased: Neurons - AAV.CAP-Mac, AAV9-X1.1 For marmosets: Broad tropism... tropism - AAV.CAP-B22 Cell type-biased: Neurons - AAV.CAP-B10 Brain vascular cells - AAV.CAP-Mac For ...: Broad tropism - AAV-PHP.eB Cell type-biased: Neurons - AAV.CAP-Mac Brain vascular cells - AAV9-X1.1,...animal Brain vascular cells - AAV9-X1.1, AAV1-X1 Neurons - AAV.CAP-B10, AAV-PHP.N Broad - AAV-PHP.AX with...

...system plasmids for CRISPR-based knock-in of EGFP-2A-PuroR cassette to the C-terminus of endogenous proteins...vector. The PITCh donor plasmid with an EGFP-2A-PuroR cassette, flanked by microhomologous sequences and...deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin (FBL...pCRIS-PITChv2-FBL - PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus Jorgensen Lab SapTrap...

..... Gateway Cloning Brain Initiative Collection NeuroMab Collection Solute carrier proteins (SLCs) are ...the Innovative Medicine Initiative (IMI) of the European Union. The RESOLUTE mission is to develop data...This Joint Undertaking receives support from the European Union's Horizon 2020 research and innovation programme...

...of the ability of a given lncRNA to cis-repress Mauro Calabrese 106253 pLS-mP-Luc Firefly Insertion of...firefly luciferase Christopher Vakoc 33307 Ubc.Luc.IRES.Puro Firefly Ubc Lentiviral expression of firefly...firefly luciferase Brent Stringer 102320 FUW-RLuc-T2A-PuroR Renilla Ubc Lentiviral Expression of renilla luciferase...luciferase Mark Kay 140328 pLenti-PGK-Venus-Fluc (puro) Firefly hPGK Lentiviral expression of Venus-firefly...

...vector. 48226 pAC94-pmax-dCas9VP160-2A-puro : dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor...

...Filaments (neuronal) Nefm EGFP Anthony Brown 32909 pEGFP-mNFM Intermediate Filaments (neuronal) Nefm EGFP...Chromatin H2B mRFP Bryan Welm, Zena Werb 21045 pH2B_mCherry_IRES_puro2 Chromatin H2B mCherry Daniel Gerlich 25999...Autophagosome LC3(ATG8) mCherry Maria Harrison 38277 pMXs-puro GFP-p62 Autophagosome Sequestosome-1 EGFP Noboru...

...R-mAbs is the NeuroMab collection - monoclonal antibodies extensively validated for neuroscience research ...research applications from the NeuroMab/Trimmer Lab Recombinant mAb Collection . Once the plasmids are transiently...

...1000000074 (Puromycin) Activation Human Zhang 3rd 3 70,290 SAM v1 - 3 plasmid system 1000000075 (Puromycin) Activation...

...marker such as puromycin, you can (also) select for the positive colonies by puromycin selection. You ...transfected cells (for example, plasmid with a puromycin resistance cassette, such as PX459 ). After you...

...Special Collections Pooled Libraries Antibodies NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection...Michael J. Fox Foundation Plasmid Resource iPSC Neurodegenerative Disease Initiative (iNDI) Collection Rett ...

...repression system can be found in this article: Vigouroux A, Oldewurtel E, Cui L, Bikard D, van Teeffelen..., 2013. DOI: 10.1021/sb400021j Reprinted from Vigouroux et al., Molecular Systems Biology, 2018. Creative...