Skip to main content
Addgene
Showing: 21 - 40 of 68 results

  1. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead...increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter...

  2. CRISPR Plasmids - Parasites

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic parasites...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the... - Toxoplasma gondii CRISPR genome-wide knockout gRNA pooled library designed with 10 guides against 8,158...

  3. CRISPR Plasmids - Tagging

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific gRNA pCRIS-PITChv2-FBL...containing Cas9 and a validated gRNA, based on the Zhang lab's PX458 . Multiple gRNA plasmids may be used. The...Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor CEBPB Human FLAG...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR system...unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence...#79888) , which expresses an untagged Cas9 AND a gRNA targeting the AAVS1 locus. Doyon Tagging Plasmid...

  4. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA in close...

  5. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...find a gRNA vector based on expression system, promoter, the type of gRNA (e.g., pegRNA, epegRNA, nicking...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing...using a prime editing guide RNA (pegRNA). As with a typical gRNA, the pegRNA is designed with a spacer that...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Prime editing is ...David Liu U6-pegRNA-H1-nick sgRNA-mCherry Mammalian, AAV hU6 + H1 pegRNA + nicking sgRNA No mCherry Hyongbum...PEmax to enhance pegRNA stability epegRNA - additional protection added to 3’ tail of pegRNA to prevent RNA...

  6. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A catalytically inactive... break (DSB) at a specific sequence. Two unique gRNAs, binding ~15-25 bp apart, are required for dCas9...

  7. CRISPR Plasmids and Resources

    Type
    Collection
    ...Empty gRNA Vectors Select an empty vector for your experiment based on a variety of factors. gRNAs gRNA... : Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links...powerful tool for genome-wide screening. RNA Targeting gRNAs RNA Targeting Type VI CRISPR systems target RNA,...nuclease generates a DSB when expressed with two gRNAs. Browse Plasmids by Model Organism Bacteria C. elegans...

  8. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully functional ...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and 2. a specific edit close to the gRNA PAM site. When a repair template is present, the...

  9. Gersbach Lab CRISPR Plasmids

    Type
    Collection
    ...Plasmid 53186 phH1-gRNA : Expresses the S. pyogenes sgRNA from the H1 promoter. 53187 pmU6-gRNA : Expresses ...pyogenes sgRNA from the mouse U6 promoter. 53188 phU6-gRNA : Expresses the S. pyogenes sgRNA from the ... human U6 promoter. 53189 ph7SK-gRNA : Expresses the S. pyogenes sgRNA from the 7SK promoter. 53190 pLV...CRISPR Cas9 gRNA plasmids from Dr. Charles Gersbach's lab. CRISPR...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts These mammalian expression...target in gene promoters by the sgRNA expressed in trans from pSPgRNA, the dCas9-VP64 fusion protein activates...activates the expression of downstream genes. Multiple sgRNAs targeted to the same promoter act synergistically...

  10. CRISPR Guide

    Type
    Collection
    ... of unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high ... guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic...commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the gRNA that is responsible for...amplify a specific sequence of DNA pegRNA P rime E dit gRNA; a specialized gRNA containing an additional primer...Cas enzyme and a gRNA specific to the gene of interest. The genomic target of the gRNA can be any ~20 nucleotide...interactions between the gRNA scaffold and surface-exposed positively-charged grooves on Cas9. gRNA binding induces...if the gRNA spacer sequence shares sufficient homology with the target DNA. Once the Cas9-gRNA complex...

  11. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To make targeted ... dead dCas9 to epigenetic modifiers. Design your gRNA to target a specific promoter or enhancer for your...

  12. CRISPR-Cas/RGN expression plasmids for human cells

    Type
    Collection
    ...RNA ( gRNA ) expression vector listed below ( MLM3636 ) can be used to construct customized gRNAs targeted...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts This page lists plasmids...available, web-based ZiFiT Targeter software. Specific gRNA expression plasmids can be built by cloning a pair...the links below: ID Plasmid 43860 MLM3636 (human gRNA expression vector) 42252 MLM3639 (Cas9 expression...

  13. Guide RNA Expression Plasmids for EGFP

    Type
    Collection
    ... the guide RNAs ( gRNAs ) used in these experiments are listed below. Note that gRNA produced from each... CRISPR Joung Lab CRISPR Plasmids EGFP gRNA CRISPR-Cas/RGN expression plasmids for EGFP You ...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The Joung Lab recently...

  14. Guide RNA Expression Plasmids for Endogenous Human Genes

    Type
    Collection
    ... the guide RNAs ( gRNAs ) used in these experiments are listed below. Note that gRNA produced from each... CRISPR Joung Lab CRISPR Plasmids Human gRNA CRISPR-Cas/RGN expression plasmids for Human genes...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The Joung Lab recently...

  15. CRISPR-Cas/RGN expression plasmids for Zebrafish

    Type
    Collection
    ...RNA ( gRNA ) expression vector listed below ( pDR274 ) can be used to construct customized gRNAs targeted...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts This page lists plasmids...available, web-based ZiFiT Targeter software. Specific gRNA expression plasmids can be built by cloning a pair...

  16. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type VI CRISPR systems...

  17. CRISPR Plasmids - Base Edit

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two classes of base...Base editors are targeted to a specific locus by a gRNA, and they can convert cytidine to uridine within...

  18. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts RNA-Guided Nucleases...consist of two components: a short ~100 nt guide RNA ( gRNA ) that uses 20 variable nts at its 5’ end to base...the target DNA. The Joung lab recently described gRNA and Cas9 expression vectors and validated the use...design of short oligonucleotides needed to construct gRNA expression vectors. More information about RGNs ...

  19. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type VI CRISPR systems...

  20. Validated gRNA Sequences

    Type
    Collection
    ... Genome Engineering CRISPR Validated gRNA Sequence Datatable Validated gRNA Target...Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA sequences that have...Does a given gRNA sequence exactly match your genomic target? Variation between a given gRNA sequence and...pyogenes, S. aureus etc.) was this gRNA sequence designed for? A given gRNA sequence may only be compatible...indicate the Cas9 application the gRNA was designed to accomplish. Validated gRNA Sequence Datatable Target Gene...details and outcomes. Submit Validated gRNA Sequences Validated gRNA sequences can be added to this table...validated gRNA sequences. Genome...
Showing: 21 - 40 of 68 results